DNA replication and gene transcription differ in their purpose, products, and templates. DNA replication occurs before cell division and produces two copies of double-stranded DNA using the original DNA as a template. Gene transcription occurs before protein translation and produces a single-stranded mRNA using the DNA as a template. DNA and RNA differ in their sugar, nitrogen base, and structure. DNA contains deoxyribose and thymine and is double-stranded, while RNA contains ribose, uracil, and is single-stranded. RNA transfers genetic information from the nucleus to the ribosome for protein creation.