DnaA/ORC: This protein is responsible for initiation of DNA replication. When DNA replication is temperature sensitive, it affects the function of Dna A/ ORC. It cannot bind to the N-terminus and thus DNA replication is immediately stopped. So with the increase in temperature, there is quick stop of the DNA replication. SSB/RPA: This is the protein complex which acts on the single stranded DNA complex. In the presences on non-permissible temperatures, this protein complex will quick stop the process of DNA replication. Thus replication fork is not formed since it requires presence of one of the proteins from the complex. It can be said that since the process is of so high order, quick stop mutation takes please due to non- permissible temperature. DnaB/Mcm: this protein complex is responsible for separating the DNA strand. With the increase in temperature, the protein cannot separate the DNA strands. Thus DNA replication stops immediately. Thus it is a quick stop type mutanation. DNA ligase: there 3 major functions of DNA ligase 1) It seals the repairs in the DNA 2) Joins the recombinant fragment and 3) it joins Okazaki fragment. When there is temperature sensitive mutants, DNA ligase will follow the process of slow stop mutants. It is because DNA replication has taken place only sealing part is left. So DNA replication will be completed but Okazaki fragments will not be joined. Solution DnaA/ORC: This protein is responsible for initiation of DNA replication. When DNA replication is temperature sensitive, it affects the function of Dna A/ ORC. It cannot bind to the N-terminus and thus DNA replication is immediately stopped. So with the increase in temperature, there is quick stop of the DNA replication. SSB/RPA: This is the protein complex which acts on the single stranded DNA complex. In the presences on non-permissible temperatures, this protein complex will quick stop the process of DNA replication. Thus replication fork is not formed since it requires presence of one of the proteins from the complex. It can be said that since the process is of so high order, quick stop mutation takes please due to non- permissible temperature. DnaB/Mcm: this protein complex is responsible for separating the DNA strand. With the increase in temperature, the protein cannot separate the DNA strands. Thus DNA replication stops immediately. Thus it is a quick stop type mutanation. DNA ligase: there 3 major functions of DNA ligase 1) It seals the repairs in the DNA 2) Joins the recombinant fragment and 3) it joins Okazaki fragment. When there is temperature sensitive mutants, DNA ligase will follow the process of slow stop mutants. It is because DNA replication has taken place only sealing part is left. So DNA replication will be completed but Okazaki fragments will not be joined..

1. DnaA/ORC: This protein is responsible for initiation of DNA replication. When DNA replication
is temperature sensitive, it affects the function of Dna A/ ORC. It cannot bind to the N-terminus
and thus DNA replication is immediately stopped. So with the increase in temperature, there is
quick stop of the DNA replication.
SSB/RPA: This is the protein complex which acts on the single stranded DNA complex. In the
presences on non-permissible temperatures, this protein complex will quick stop the process of
DNA replication. Thus replication fork is not formed since it requires presence of one of the
proteins from the complex. It can be said that since the process is of so high order, quick stop
mutation takes please due to non- permissible temperature.
DnaB/Mcm: this protein complex is responsible for separating the DNA strand. With the
increase in temperature, the protein cannot separate the DNA strands. Thus DNA replication
stops immediately. Thus it is a quick stop type mutanation.
DNA ligase: there 3 major functions of DNA ligase 1) It seals the repairs in the DNA 2) Joins
the recombinant fragment and 3) it joins Okazaki fragment. When there is temperature sensitive
mutants, DNA ligase will follow the process of slow stop mutants. It is because DNA replication
has taken place only sealing part is left. So DNA replication will be completed but Okazaki
fragments will not be joined.
Solution
DnaA/ORC: This protein is responsible for initiation of DNA replication. When DNA replication
is temperature sensitive, it affects the function of Dna A/ ORC. It cannot bind to the N-terminus
and thus DNA replication is immediately stopped. So with the increase in temperature, there is
quick stop of the DNA replication.
SSB/RPA: This is the protein complex which acts on the single stranded DNA complex. In the
presences on non-permissible temperatures, this protein complex will quick stop the process of
DNA replication. Thus replication fork is not formed since it requires presence of one of the
proteins from the complex. It can be said that since the process is of so high order, quick stop
mutation takes please due to non- permissible temperature.
DnaB/Mcm: this protein complex is responsible for separating the DNA strand. With the
increase in temperature, the protein cannot separate the DNA strands. Thus DNA replication
stops immediately. Thus it is a quick stop type mutanation.
DNA ligase: there 3 major functions of DNA ligase 1) It seals the repairs in the DNA 2) Joins
the recombinant fragment and 3) it joins Okazaki fragment. When there is temperature sensitive
mutants, DNA ligase will follow the process of slow stop mutants. It is because DNA replication

2. has taken place only sealing part is left. So DNA replication will be completed but Okazaki
fragments will not be joined.