Successfully reported this slideshow.
We use your LinkedIn profile and activity data to personalize ads and to show you more relevant ads. You can change your ad preferences anytime.

Blastocystis hominis


Published on

Published in: Health & Medicine, Technology
  • Be the first to comment

Blastocystis hominis

  1. 1. Wet mount
  2. 2.  Initially described by Prowasek and Alexeieff, then named by Brumpt in 1912 Blastocystis hominis is an inhabitant of the lower intestinal tract of humans and other animals Formerly classified as a yeast under the genus Schizosaccharomyces, while other taxonomists considered it to be related to Blastomyces
  3. 3.  Lacks a cell wall but has a mitochondrion with protozoan morphology. Capable of pseudopodial extension and retraction Asexual reproduction by binary fission or sporulation under strict anaerobic conditions Optimal growth= 37 oC in the presence of bacteria; does not grow on fungal media
  4. 4. Parasite Biology Multiplication of Blastocystis hominis is by binary fission Transmitted by fecal-oral route Morphological forms: 1. Vacuolated 2. Ameba-like 3. Granular 4. Multiple fission 5. Cyst 6. Avacuolar
  5. 5. Vacuolated form Most predominant form in fecal specimen Spherical in shape, measuring 5-10 µm in diameter Large central vacuole pushes the cytoplasm and nuclei to the periphery of the cell Sometimes, very thick capsule surround them Prominent central vacuole has been found to be a reproductive organelle Main type of Blastocystis causing diarrhea
  6. 6. Ameba-like forms Occasionally observed in stool samples Exhibit active extension and retraction of pseudopodia The nuclear chromatin, when visible, characteristically shows peripheral clumping Appears to be an intermediate stage between the vacuolar and pre-cystic form Allows the parasite to ingest bacteria to enhance encystment
  7. 7. Granular forms Mainly observed from old cultures Diameter of the cell varies from 10 -60 µm Granular contents develop into daughter cells of the ameba-form when the cell ruptures
  8. 8. Multiple Fission Arise from vacuolated forms It is believed that these multiple fission forms produce many vacuolated forms.
  9. 9. Cystic form Size in about 3-55 µm Has a very prominent and thick osmophilic electron dense wall Appears as a sharply demarcated polymorphic, but mostly oval or circular, dense body surrounded by a loose outer membranous layer (seen in contrast microscopy) Membranous layer corresponds to the fibrillar layer described around the cyst at ultrastructural level; easiest diagnostic feature
  10. 10.  It is assumed that the thick-walled cyst might be responsible for external transmission while those cysts with thin walls might be the cause of re-infection within a host’s intestinal tract
  11. 11. Pathogenesis and ClinicalManifestation Blastocystosis- infection with Blastocystis hominis Blastocystis hominis as a cause of gastrointestinal pathology is controversial Several studies showed that the presence of parasite in a majority of patients was not associated with symptoms, or is found with other organisms that are more likely to be cause of the symptoms
  12. 12.  Other studies concluded that presence of Blastocystis in large numbers produce a wide variety of intestinal disorders: abdominal cramps, irritable bowel syndrome, bloating, flatulence, mild to moderate diarrhea without fecal leucocytes or blood, nausea, vomiting, low grade fever, and malaise Symptoms usually last about 3-10 days, but may sometimes persist for weeks or months
  13. 13.  It has been found out that in subjects suffering from Blastocystis showed a significant association with gastrointestinal symptoms Other studies also provided evidence of cellular immune function changes in infected individuals
  14. 14. Diagnosis Laboratory detection from stool is needed for confirmation Stool samples should be collected more than once from patients showing signs and symptoms Microscopic examination using direct fecal smear is useful but sensitivity is increased when concentration techniques are used
  15. 15.  Hematoxylin or trichome staining offers a very convenient and easy method to differentiate the various stages of Blastocystis Leukocytes are usually seen in fecal smears and stool eosinophilia may also be observed The organism can be cultured using the Boeck and Drbohlav’s or the Nelson and Jones media
  16. 16. Treatment Blastocystis is difficult to erradicate Hides in the intestinal mucus, sticks and holds on the intestinal membranes Drug of choice is metronidazole given at 750 mg 3x daily for 10 days (Pedriatric dose: 35-50 mg per kg per day in 3 doses for 5 days) or iodoquinol given at 650 mg 3x daily for 20 days.
  17. 17.  However, there are reported cases of resistance of Blastocystis to metronidazole Trimethroprim-sulfamethaxazole (TMP-SMX) has been found to be highly effective against Blastocystis
  18. 18. Epidemiology Reported practically worldwide, with infections common in tropical , subtropical and developing countries In general, studies from developed countries report approx. 1.5% - 10% overall prevalence of Blastocystis hominis All ages are affected but symptomatic cases are commonly found in children and in those with weakened immune systems
  19. 19.  A prevalence of up to 11.6% was reported from Stanford University Hospital Occurrence of parasite in temperate countries is generally associated with recent travel to the tropics and consumption of untreated drinking water, an indication that infection is possibly through the oral route and it is more likely to occur in crowded and unsanitary conditions
  20. 20.  Outbreaks of Blastocystis hominis in day care centers were reported in Spain, Brazil and Canada In the Philippines, results of 355 stools examined in 1997 by the Department of Parasitology, College of Public Health, University of the Philippines Manila, showed a prevalence of 22.8% with or without other intestinal parasites or organisms
  21. 21.  In 1988, the prevalence was 20.7% in 772 stool samples examined. Studies have shown prevalence rates of 40.6% among food service workers in a tertiary hospital and 23.6% among food handlers in selected school canteens in Manila
  22. 22.  Several animals like the pig-tailed macaques, chicken, dogs, and ostriches harbor Blastocystis similar to those found in humans. Evidence also shows that it is present in house lizards and cockroaches, thus implying that food and water contaminated by fecal droppings of these “home visitors” may transmit Blastocystis
  23. 23. Prevention and Control Disease can be prevented by consuming safe drinking water Provisions for sanitary preparation may be of value in efforts to prevent and control this infection The cysts of Blastocystis hominis can survive up to 19 days in water at normal temperature and have shown resistance to chlorine at the standard concentrations