The sequences cut by each restriction enzyme are shown below. Note that only one strand of DNA sequence is shown, but each of these restriction enzymes cuts double stranded DNA sequences. The slashes in these sequences indicate the position where the indicated restriction enzyme cuts. BamHI: 5 AT/CGAT 3 AccI: 5 G/GATCC 3 BbuI: 5 C/TCGAG 3 XhoI: 5 GT/CGAC 3 EcoRI: 3 G/CATGC 5 ClaI: 3 CTTAA/G 5 BstI: 3 CCTAG/G 5 Which lane on the gel represents the results you would expect to see if you cut your vector with ClaI? Which lane on the gel represents the results you would expect to see if you cut your vector with BamHI and EcoRI? Suppose you cut your sec13 gene out of the human genome with ClaI but you ran out of ClaI before you could cut your plasmid. What other restriction enzyme listed could you use to cut your plasmid so that you could insert your gene into the cut plasmid? Suppose you cut your sec13 gene out of the human genome with BbuI but you ran out of BbuI before you could cut your plasmid. What other restriction enzyme listed could you use to cut your plasmid so that you could insert your gene into the cut plasmid?.

1. The sequences cut by each restriction enzyme are shown below. Note that only one strand of
DNA sequence is shown, but each of these restriction enzymes cuts double stranded DNA
sequences. The slashes in these sequences indicate the position where the indicated restriction
enzyme cuts.
BamHI: 5 AT/CGAT 3 AccI: 5 G/GATCC 3 BbuI: 5 C/TCGAG 3 XhoI: 5 GT/CGAC 3 EcoRI:
3 G/CATGC 5 ClaI: 3 CTTAA/G 5 BstI: 3 CCTAG/G 5
Which lane on the gel represents the results you would expect to see if you cut your vector with
ClaI?
Which lane on the gel represents the results you would expect to see if you cut your vector with
BamHI and EcoRI?
Suppose you cut your sec13 gene out of the human genome with ClaI but you ran out of ClaI
before you could cut your plasmid.
What other restriction enzyme listed could you use to cut your plasmid so that you could insert
your gene into the cut plasmid?
Suppose you cut your sec13 gene out of the human genome with BbuI but you ran out of BbuI
before you could cut your plasmid. What other restriction enzyme listed could you use to cut
your plasmid so that you could insert your gene into the cut plasmid?