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Q6: You have constructed phospholipid vesicles that contain Na+/K+ pumps as the sole
membrane protein. The portion of the pump that normally faces the cytosol is oriented towards
the outside of the vesicles. The solutions inside the vesicles and outside the vesicles have only
Na+ ions and NOK+ ions, and ATP is present only on the outside of the vesicle. What would
happen?
Na+ would be pumped repeatedly out of the vesicle. Na+ would be pumped repeatedly into the
vesicle. Very little Na+ would move into the vesicle (only one partial cycle of all the pumps
would occur). Na+ would be pumped into and out of the vesicle at the same rate, so there would
be no net movement of Na+. No Na+ at all would move.

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Q6 You have constructed phospholipid vesicles that contain Na+K+ pu.pdf

  • 1. Q6: You have constructed phospholipid vesicles that contain Na+/K+ pumps as the sole membrane protein. The portion of the pump that normally faces the cytosol is oriented towards the outside of the vesicles. The solutions inside the vesicles and outside the vesicles have only Na+ ions and NOK+ ions, and ATP is present only on the outside of the vesicle. What would happen? Na+ would be pumped repeatedly out of the vesicle. Na+ would be pumped repeatedly into the vesicle. Very little Na+ would move into the vesicle (only one partial cycle of all the pumps would occur). Na+ would be pumped into and out of the vesicle at the same rate, so there would be no net movement of Na+. No Na+ at all would move.