Featuring: Cryopreservation Specialist, Michael George This webinar will introduce cell storage equipment and techniques in order to provide the most up-to-date trends in research today. There will be special emphasis on human primary cell storage, cryopreservation, and transportation. Topics to be covered in the webinar: • Background on life science storage methods • Advantages/disadvantages of storage equipment • What not to do (common mistakes/misconceptions) • Interview with James Lee, Associate Director of Production, AllCells • Q&A

1. Your Reliable Source of Human Primary Cells
Cell Storage Best Practices:
Techniques to Ensure Greater Research
Results
AllCells Exclusive Webinar Event
9/17/2015 12pm PST
Featuring Mike George, Cryopreservation Specialist

2. ConfidentialYour Reliable Source of Human Primary Hematopoietic Cells and Services
Outline
•Introduction
•Life science storage methods
•Comparison of equipment
•What not to do (common mistakes/misconceptions)
•Discussion with James Lee – Storage of Primary Cells
•Q&A

3. ConfidentialYour Reliable Source of Human Primary Hematopoietic Cells and Services
Introduction
•Mike George, Cryopreservation Specialist
• Owner of MG Lab Solutions Inc
• 17 years experience specifying, selling and
servicing cryogenic equipment
• Other equipment to meet laboratory and storage
needs

4. ConfidentialYour Reliable Source of Human Primary Hematopoietic Cells and Services
Background on storage methods
•Material can be stored in -80c, liquid nitrogen vapor or
liquid nitrogen liquid
•Determine your temperature requirements based on
the material to be stored
•Will this be short term or long term storage?
•Glass Transition Temperature

5. ConfidentialYour Reliable Source of Human Primary Hematopoietic Cells and Services
Comparison of storage equipment

6. ConfidentialYour Reliable Source of Human Primary Hematopoietic Cells and Services
Comparison of storage equipment
•Select your storage vessel and inventory
system
•Personal Protective Equipment (PPE)
•Ultra-Low (-80c) Storage
•Liquid Nitrogen Storage – Liquid Phase
•Liquid Nitrogen Storage – Vapor Phase
•How to prepare your samples for storage
•Cost of Equipment

7. ConfidentialYour Reliable Source of Human Primary Hematopoietic Cells and Services
What not to do
•Ignore maintenance
•Do not plan ahead for inventory safeguards i.e.,
data tracking, temperature monitoring, storage
tools and safety training
•Assume all is fine – check temperatures, viability
and content

8. ConfidentialYour Reliable Source of Human Primary Hematopoietic Cells and Services
Storage of human primary cells
•James Lee, Associate Director Production, AllCells
• 16 years primary cells experience
• Specializing in cell isolation, culture

9. ConfidentialYour Reliable Source of Human Primary Hematopoietic Cells and Services
Storage of human primary cells
•Special considerations for storage of human primary
cells vs cell lines, etc?
•Collection
•Processing
•Freezing down at control rate
•Work quickly

10. ConfidentialYour Reliable Source of Human Primary Hematopoietic Cells and Services
Storage of human primary cells
•Process and equipment setup at AllCells lab
•Presentation of AllCells tank data

11. ConfidentialYour Reliable Source of Human Primary Hematopoietic Cells and Services
Storage of human primary cells
•Cryo media discussion
•Different solutions
•AllCells VitalCellsTM

12. ConfidentialYour Reliable Source of Human Primary Hematopoietic Cells and Services
Transportation of human primary cells
•Transport vessel options for shipment of cells:
• Dry ice/liquid nitrogen dry shippers
•International, long distance shipments
•Timing considerations
• Domestic
• International
•Courier options

13. ConfidentialYour Reliable Source of Human Primary Hematopoietic Cells and Services
Q&A
 Please use the prompt on the right of your screen to
send a question to the organizer
 Questions will be read and answered
 Any offline questions may be sent to
sales@allcells.com

14. ConfidentialYour Reliable Source of Human Primary Hematopoietic Cells and Services
 Mike George
Cryopreservation Specialist, MG Lab Solutions
Please direct offline questions to
mgeorge@mglabsolutions.com
510-610-3656
 James Lee
Associate Director of Production, AllCells
Please direct offline questions to jlee@allcells.com or
sales@allcells.com
www.allcells.com/technical-support/
www.allcells.com/support/webinars/
Contact Information

15. ConfidentialYour Reliable Source of Human Primary Hematopoietic Cells and Services
Following Webinar
Email to follow with information on how to obtain:
•a free sample bottle of AllCells VitalCells™ Cryo Media
solution
•a free vial of our high quality Frozen PBMC product
•an exclusive 10% discount coupon eligible for a future
purchase of any of our popular Frozen PBMC products:
PB003F, PB004F, PB005F, PB006F