1. What are some potential pitfalls in using a bacterial 2 hybrid (or Yeast 2 hybrid) system to study protein protein interactions? How could you address them? 2. Why is the transcription attenuation system of the trp operon something that cannot happen in eukaryotes? 3. Let's compare the Iac, ara, trp operons. Why do you think there are differences in the ways these three sets of genes are regulated?.