1. Does WiFi Radiation
Affect D. tigrina and
R. Pipiens?
Tomomi Shore, George James, Shawn Cyran, and Crystal Ponticello

2. What is WiFi Radiation?
● International Agency for Research on Cancer- Class 2B
● Electromagnetic Radiation Spectrum
● Ionizing vs nonionizing
● Electromagnetic Hypersensitivity Syndrome Real?
● Wireless Fidelity
● School case
● Unsupported
● Carcinogenic/Oxidative stress on DNA?
● Antioxidants → Vitamin C

3. What is Rana pipiens?
●AKA Northern leopard frogs.
●Eats plants and small animals.
●Similar genes with humans.

4. What is Dugesia tigrina?
●Platyhelminthes phylum “flat worm”
●Similar to humans
●Regeneration ex: epimorphosis
●Dthox genes
Head Body

5. Hypothesis
• We hypothesized that higher exposure to WiFi radiation will
hinder growth and development of both of the organisms.
• Greater exposure to WiFi radiation would mutate Dthox
genes more frequently, so D. tigrina exposed to more WiFi
radiation would not regenerate as normally as those
exposed to less WiFi radiation.
• We hypothesized that Vitamin C would negate the
detrimental effects of WiFi radiation.

6. D. tigrina:
Numb &
amputate
heads.
Measure
length of
pieces
Experimental
Groups: Low
Dose, High
Dose, and High
dose with
Vitamin C
Control Group:
Faraday Cage
High dose
placed next to
router (105.1
mW/m2)
Low Dose
46cm away
(0.619
mW/m2)
Measure length of
D. tigrina pieces
for 6 days.
R. pipiens
maturation stages
and remaining
recorded every
week for 6 weeks.
Methodology

7. Control Low
Dose
High Dose w/
Vitamin C
High
Dose
Total
33% 0% 16% 33% 20%
Results

8. Control Low
Dose
High Dose w/
Vitamin C
High
Dose
Total
66% 16% 50% 33% 41%

10. The Effect of WiFi Radiation on D. Tigrina on Head
Pieces Growth (Trial 1)
Day High
Dose
(mm)
High w/
Vitamin C
(mm)
Low
(mm)
Control
(mm)
1 0.03 0.46 0.12 0.11
2 0.16 0.79 0.46 0.18
3 0.25 0.83 0.46 0.45
5 0.6 0.93 0.66 0.59

11. Day High
Dose
(mm)
High w/
Vitamin C
(mm)
Low
(mm)
Control
(mm)
1 0.62 1.38 0.33 0.06
2 0.63 1.36 0.66 0.46
3 1.12 1.39 0.46 0.51
5 1.13 2.66 1.68 1.68

12. Day High Dose
(mm)
Low
(mm)
Control
(mm)
1 0.542 0.5 -0.137
2 0.667 0.6 0.1
3 0.677 0.81 0.37
4 0.8 0.78 N/A
5 0.967 0.883 N/A

13. Day High Dose
(mm)
Low
(mm)
Control
(mm)
1 0.583 0.2 0.467
2 1.25 0.35 0.5
3 1.602 0.527 0.847
4 1.602 0.827 N/A
5 1.603 N/A N/A

14. Conclusion
●Hypothesis that higher exposure to WiFi radiation reduce
growth unsupported
●WiFi radiation showed no significant effect on D. tigrina
(no anatomical deformities found) and R. pipiens
●Vitamin C shown to have effect on D. tigrina because of
repairing properties but none on R. pipiens.
●Previous studies didn't use these organisms.
●Nonionizing effects.
●Be cautious, but not overly concerned.

15. Future Direction
●Exploring the effect of higher doses of radiofrequency radiation
on other animals.
●Focusing on the frequency of cancer and tumors in animals.
●Human subjects may be also used to further explore if
Electromagnetic Hypersensitivity is a disorder that should be of
concern, especially for children.
●Measuring frequency of Dthox genes in D. tigrina exposed to
varying amounts of WiFi radiation using a quantative PCR.