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Mechanical assessment of 
tripled hamstring tendon 
when using suspensory 
fixation for cruciate ligament 
reconstruction 
DR. GEETHAN I MS(ORTH) DNB(ORTH)
Triple = trouble?
Co-Authors 
 Dr. David V Rajan 
 Dr. Clement J Joseph 
 Dr. Santhosh Sahanand 
 Dr. Ashwin Vijay 
No conflicts of interest in the authorship and presentation of 
this paper.
Background 
 ACL reconstruction is one of the commonly performed arthroscopic 
procedures 
 Hamstring graft is used by many surgeons 
An international survey on anterior cruciate 
ligament reconstruction practices 
Chechik et al, SICOT 2013
Why triple? 
 Usual length of semitendinosus tendon is 28 – 
32 cm. 
 Double 15 cm 
 Triple 10 cm 
 Quadruple 7-8 cm 
2 cm 
4 cm 
2.5- 3 cm 
Total 9 cm 
Chiang et al, KSSTA 2012 
Triple without trouble, IASCON 2014
Lavery et al, Arthroscopy Techniques 2014 (3) 
Lee KSSTA 2013 
Snow et al KSSTA 2012 
Strobel. Manual of 
Arthroscopy 
Triple without trouble, IASCON 2014
What’s the trouble? 
 The preferred femoral fixation option is Suspensory fixation 
Triple without trouble, IASCON 2014
Snow et al, KSSTA 2012 
 Compared tripled tendon to 
double 
 Found no benefit with triple 
spite of increased thickness 
 Recommended against tripling 
Triple without trouble, IASCON 2014
Many methods of tripling 
Snow et al KSSTA 2012 
Lavery et al, Arthroscopy Techniques 2014 (3) 
Triple without trouble, IASCON 2014 
Lee KSSTA 2013 
Strobel. Manual of 
Arthroscopy
 Purpose 
 To compare to mechanical properties of tripled graft prepared 
by three different methods 
 Hypothesis 
 Tripled graft will have variable mechanical properties 
depending on the way of preparation 
Triple without trouble, IASCON 2014
Materials 
Similar structural and mechanic properties as young adult 
Bovine hind foot hoof extensor tendon 
Triple without trouble, IASCON 2014
Tripled Graft Configurations 
Group I 
Group II Group III Group IV
Test Construct 
• InstronMaterial testing 
system- fully automated 
• All parameters entered into 
software 
• All data measured by the 
system and read off the 
computer screen, and 
output print out 
Triple without trouble, IASCON 2014
Mechanical assessment 
Test Protocol 
 Preloading 
 10 to 50 N at 0.1 Hz for 10 cycles 
 Cyclic Loading 
 1000 cycles ; 50 and 250N at a frequency of 0.5 Hz. 
 Distraction to failure 
 50mm/min 
As reported by Petre et al, AJSM; 2013
Results
Displacement 
 Group I Groups 1.13 ± 0.11 Observed mm 
‘t’ 
 Group II 4.91 ± value 
0.49 mm 
Displacement I Vs. II 16.92 3.355 ( p = 0.01) p < 0.01 
 Group III 1.822 ± 0.55 mm 
I Vs. III 11.15 3.355 ( p = 0.01) p < 0.01 
 Group IV 1. 126 ± .018 mm 
Table ‘t’ value ‘p’ value 
I Vs. IV 0.044 1.806 ( p = 0.10) P > 0.10 
 All samples survived cyclical loading 
3mm is considered acceptable displacement 
Daniel DM, Stone ML, Sachs R, Malcom L. Instrumented measurement of anterior 
knee laxity in patients with acute anterior cruciate ligament disruption. Am J Sports 
Med. 1985;13(6):401-407
Ultimate load at failure 
Groups Observed ‘t’ 
Table ‘t’ value ‘p’ value 
 Group I 957 ± 23.30 N 
value 
 Group II 590.8 ± 26.55 N 
I Vs. II 24.27 3.355 ( p = 0.01) p < 0.01 
 Group III 682.6 ± 59.28 N 
 Group IV 963.4 ± 21.72 N 
Load at 
failure 
I Vs. III 9.63 3.355 ( p = 0.01) p < 0.01 
I Vs. IV 0.45 1.806 ( p = 0.10) P > 0.10 
Grafts may be subjected to loads of 
upto 600N during normal rehabilitation 
Ettinger M, Petri M, Kwisda S, Krettek C, Jagodzinski M. Biomechanical 
Considerations for Graft Fixation in ACL Reconstruction. Techniques in Orthopaedics. 
2013;28(2): 126-132.
Mode of failure 
Group I Group IV 
Triple without trouble, IASCON 2014
Mode of Failure 
Group II Group III
Prior studies…Snow et al, KSSTA 
2012 
 No difference between double and triple 
 All the failures at interference screw 
 Uneven distribution of load across three strands 
Triple without trouble, IASCON 2014
Advantages of this study 
 Compares three different techniques of tripling 
 Isolated the graft-loop interface 
 Suspensory fixation device, Graft thickness and 
length standardised 
Triple without trouble, IASCON 2014
Limitations 
 Tibial fixation is with a vice grip 
 In vitro study 
 Loading represents extreme case scenario 
 Unidirectional loading 
 Role of graft healing is not studied 
 Stiffness is not reported 
 Differential loading is not studied 
Triple without trouble, IASCON 2014
Conclusions 
 Hypothesis that tripled tendon configuration have variable 
mechanic properties is found true. 
 Triple strand configurations studied in Group II and Group III 
in our study are significantly mechanically inferior. 
 Suturing the three strands together as in Group IV has 
equivalent mechanical properties as quadrupled strand in 
our controlled laboratory study 
Triple without trouble, IASCON 2014
Triple without trouble? 
 What this study tells the surgeon? 
 Tripled grafts have variable mechanical properties and the 
surgeon should consider the inferior mechanical properties 
while choosing to triple graft. 
 Surgeon should consider suturing the three strands of graft 
together increase ultimate failure load.
Thank You

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Mechanical Assessment of Tripled Hamstring Tendon When Using Suspensory Fixation for Cruciant Ligament Reconstruction-Dr. I. Geethan

  • 1. Mechanical assessment of tripled hamstring tendon when using suspensory fixation for cruciate ligament reconstruction DR. GEETHAN I MS(ORTH) DNB(ORTH)
  • 3. Co-Authors  Dr. David V Rajan  Dr. Clement J Joseph  Dr. Santhosh Sahanand  Dr. Ashwin Vijay No conflicts of interest in the authorship and presentation of this paper.
  • 4. Background  ACL reconstruction is one of the commonly performed arthroscopic procedures  Hamstring graft is used by many surgeons An international survey on anterior cruciate ligament reconstruction practices Chechik et al, SICOT 2013
  • 5. Why triple?  Usual length of semitendinosus tendon is 28 – 32 cm.  Double 15 cm  Triple 10 cm  Quadruple 7-8 cm 2 cm 4 cm 2.5- 3 cm Total 9 cm Chiang et al, KSSTA 2012 Triple without trouble, IASCON 2014
  • 6. Lavery et al, Arthroscopy Techniques 2014 (3) Lee KSSTA 2013 Snow et al KSSTA 2012 Strobel. Manual of Arthroscopy Triple without trouble, IASCON 2014
  • 7. What’s the trouble?  The preferred femoral fixation option is Suspensory fixation Triple without trouble, IASCON 2014
  • 8. Snow et al, KSSTA 2012  Compared tripled tendon to double  Found no benefit with triple spite of increased thickness  Recommended against tripling Triple without trouble, IASCON 2014
  • 9. Many methods of tripling Snow et al KSSTA 2012 Lavery et al, Arthroscopy Techniques 2014 (3) Triple without trouble, IASCON 2014 Lee KSSTA 2013 Strobel. Manual of Arthroscopy
  • 10.  Purpose  To compare to mechanical properties of tripled graft prepared by three different methods  Hypothesis  Tripled graft will have variable mechanical properties depending on the way of preparation Triple without trouble, IASCON 2014
  • 11. Materials Similar structural and mechanic properties as young adult Bovine hind foot hoof extensor tendon Triple without trouble, IASCON 2014
  • 12. Tripled Graft Configurations Group I Group II Group III Group IV
  • 13. Test Construct • InstronMaterial testing system- fully automated • All parameters entered into software • All data measured by the system and read off the computer screen, and output print out Triple without trouble, IASCON 2014
  • 14. Mechanical assessment Test Protocol  Preloading  10 to 50 N at 0.1 Hz for 10 cycles  Cyclic Loading  1000 cycles ; 50 and 250N at a frequency of 0.5 Hz.  Distraction to failure  50mm/min As reported by Petre et al, AJSM; 2013
  • 16. Displacement  Group I Groups 1.13 ± 0.11 Observed mm ‘t’  Group II 4.91 ± value 0.49 mm Displacement I Vs. II 16.92 3.355 ( p = 0.01) p < 0.01  Group III 1.822 ± 0.55 mm I Vs. III 11.15 3.355 ( p = 0.01) p < 0.01  Group IV 1. 126 ± .018 mm Table ‘t’ value ‘p’ value I Vs. IV 0.044 1.806 ( p = 0.10) P > 0.10  All samples survived cyclical loading 3mm is considered acceptable displacement Daniel DM, Stone ML, Sachs R, Malcom L. Instrumented measurement of anterior knee laxity in patients with acute anterior cruciate ligament disruption. Am J Sports Med. 1985;13(6):401-407
  • 17. Ultimate load at failure Groups Observed ‘t’ Table ‘t’ value ‘p’ value  Group I 957 ± 23.30 N value  Group II 590.8 ± 26.55 N I Vs. II 24.27 3.355 ( p = 0.01) p < 0.01  Group III 682.6 ± 59.28 N  Group IV 963.4 ± 21.72 N Load at failure I Vs. III 9.63 3.355 ( p = 0.01) p < 0.01 I Vs. IV 0.45 1.806 ( p = 0.10) P > 0.10 Grafts may be subjected to loads of upto 600N during normal rehabilitation Ettinger M, Petri M, Kwisda S, Krettek C, Jagodzinski M. Biomechanical Considerations for Graft Fixation in ACL Reconstruction. Techniques in Orthopaedics. 2013;28(2): 126-132.
  • 18. Mode of failure Group I Group IV Triple without trouble, IASCON 2014
  • 19. Mode of Failure Group II Group III
  • 20. Prior studies…Snow et al, KSSTA 2012  No difference between double and triple  All the failures at interference screw  Uneven distribution of load across three strands Triple without trouble, IASCON 2014
  • 21. Advantages of this study  Compares three different techniques of tripling  Isolated the graft-loop interface  Suspensory fixation device, Graft thickness and length standardised Triple without trouble, IASCON 2014
  • 22. Limitations  Tibial fixation is with a vice grip  In vitro study  Loading represents extreme case scenario  Unidirectional loading  Role of graft healing is not studied  Stiffness is not reported  Differential loading is not studied Triple without trouble, IASCON 2014
  • 23. Conclusions  Hypothesis that tripled tendon configuration have variable mechanic properties is found true.  Triple strand configurations studied in Group II and Group III in our study are significantly mechanically inferior.  Suturing the three strands together as in Group IV has equivalent mechanical properties as quadrupled strand in our controlled laboratory study Triple without trouble, IASCON 2014
  • 24. Triple without trouble?  What this study tells the surgeon?  Tripled grafts have variable mechanical properties and the surgeon should consider the inferior mechanical properties while choosing to triple graft.  Surgeon should consider suturing the three strands of graft together increase ultimate failure load.