Interferon, Interleukin, Chemokines, Haemetopoietin, Tumor Necrotic Factor, Colony Stimulating Factor, Transforming Growth Factor

1. Taha Nazir PhD (Microbiology)
Microbiology, Cell Pathology & Molecular Biology
Research Group. E.: taha@icdtdi.ca
ANALYTICAL REVIEW OF CYTOKINES
Interferon, Interleukin,
Chemokines, Haemetopoietin,
Tumor Necrotic Factor, Colony
Stimulating Factor, Transforming
Growth Factor

2. • Cytokines are immunomodulating agent or
group of proteins made by immune system
that act as chemical messenger.
• Cytokines are cell signaling molecules that
aid cell to cell communication in immune
responses and stimulate the movement of
cells towards sites of inflammation, infection
and trauma.

3. • Interferon (IFN)
• Interleukin (IL)
• Chemokines (CK)
• Haemetopoietin
• Tumor Necrotic Factor (TNF)
• Colony Stimulating Factor (CSF)
• Transforming Growth
Factor (TGF)

4. • Interferon family Antiviral proteins
• Interleukin family variety of actions
dependent upon
interleukin and cell type
• Chemokine family Direct cell migration ,
adhension and activation

5. • Haemetopoietin Promote cell
proliferation and
differentiation
• Tumor necrosis Regulate inflammatory
factor family and immune response
• Transforming growth Regulation of immune
Factor cells

6. Particulate removal
concentration
Capture / initial purification
Intermediate purification
Final purification
Sterilization /formulation

7. • Interferon (IFN) is type of cytokines produced
by immune system in response to challenges
such as virus, tumor or parasites etc.

8. • Recombinant forms of α Interferon
 α 2a
 α 2b
• Recombinant forms of β Interferon
 β 1a
 β 1b
• Recombinant forms of γ Interferon
 γ 1b

9. INTERFERON BRAND NAME COMPANY
Αlpha 2a Roferon 3 MIU
Roferon 4.5 MIU
Roferon 6 MIU
Roferon 9 MIU
Roche
Alpha 2b Intron A 10 MIU/ml Merck Sharp & Dohme
Beta 1a Rebif 22 mcg
Rebif 44 mcg
Avonex 30microorganism /0.5 ml
Biogen
Merck
Beta 1b Betaferon Bayer
Extavia Novartis
Gamma 1b Immukin Boehringer Ingelheim
peginterferon
alfa-2a
Pegasys 90 mcg
Pegasys 135 mcg
Pegasys 180 mcg
Roche

11. • Interleukin (IL) is a group of cytokines with
complex immunomodulatory function and
released from leukocytes.
• It stimulate production of natural killer cells
and cytotoxic T-cells that can destroy tumor
cells.

12. INTERLEUKIN BRAND NAME COMPANY
Atlizumab / Tocilizumab
/ IL-6
RoActemra 20 mg/ml Roche
IL-2R α / Basiliximab Simulect 10mg
Simulect 20mg
Novartis
Aldesleukin / IL-2 Proleukin Novartis
Canakinumab / IL-1 Ilaris 150mg Novartis
Mepolizumab / IL-5 Nucala 100 mg GlaxoSmithKline

14. Abbreviations:
F Filtration
UF Ultra filteration
DF Distilled filtration
hIL human interleuken
MBP-hIL Myelin Basic Protiens human IL
TEV Protease Tobacco Etch Virus

15. • https://www.medicines.org.uk/emc/search
• https://www.pharma.us.novartis.com/produc
t/pi/pdf/simulect.pdf
• http://mcl1.ncifcrf.gov/waugh_pubs/46_Wau
gh.pdf