1. Stephanie Chantelle Bannister
Burggasse 22/2/25, Vienna, Austria, A-1070
stephanie.bannister@univie.ac.at - Tel. +43(0) 6767 339955
Nationality: Australian
Date of Birth: 06.08.1984
Residency: Austria (Rot-Weiß-Rot Plus; 2011 - current)
Languages: English (native speaker), German (Intermediate B1 level), Japanese (Basic)
Current Position
University Assistant (Postdoctoral Researcher) 20114 - 2016
TessmarGroup: Departmentof Microbiology,ImmunobiologyandGenetics
The Universityof Vienna, Max F.PerutzLaboratoriesGmbH,ViennaBioCenterCampus.
Contact: Dr. Kristin Tessmar-Raible [Ph:+43 1 4277 74635]
Education
2007-2011 Doctor of Philosophy (PhD: Biological Sciences)
The Universityof Melbourne &
AustralianCommonwealthScientificandIndustrial ResearchOrganisation(CSIRO)
2006-2007 Honours: Bachelor Science (Biotechnology)
DeakinUniversity&
AustralianCommonwealthScientificandIndustrial ResearchOrganisation(CSIRO)
2003-2005 Bachelor of Science (Biotechnology) (Distinction)
DeakinUniversity
Research Experience
2014-2016 University Assistant (Postdoctoral Researcher)
Tessmar Group: Max F. PerutzLaboratories, The UniversityofVienna(Austria)
 I lead a collaborative project involving colleagues, bioinformaticians, mass spectrometry
and RNA sequencing facilities, and using quantitative transcriptomics and proteomics to
investigatesystem-levelmolecularchangesinthe brainof a marine bristle worm.
 Mentored colleagues and managed optimization of protocols and pipelines for genome
editing,mutationscreening,transcriptomic&proteomicdataanalysis.
 Developed animal behavior assays and tracking software in collaboration with the
KropatschPatternRecognitionandImage ProcessingGroupat the TU wien.
Contact: Dr. KristinTessmar-Raible[+431 4277 74635; kristin.tessmar@mfpl.ac.at]
2011 - 2014 VIPS Postdoctoral Research Fellow
Raible Group: Max F. PerutzLaboratories, The UniversityofVienna(Austria)
 I was the first to establish targeted genome editing techniques (TALEN & CRISPR/Cas9)
for the marine bristle worm P.dumerilii anddevelopedprotocolsfortwofishspecies:
 Collaborated with colleagues in Germany to identify a link between estrogen receptor
expressionandestablishmentof the germline duringembryogenesisin P.dumerilii.

2.  I was awarded a young career researchertravel award to presentthis work at the 2014
FASEB Conference onGenome Engineering. Thisprojectwassupportedby aVIPS
Fellowship.
Contact: Dr. FlorianRaible [+431 4277 54616; florian.raible@mfpl.ac.at]
2007 - 2011 PhD Research Student
The UniversityofMelbourne & CSIRO Australian Animal Health Laboratories
(Australia)
Thesis:Characterisation of microRNA expressionduringgonadal sexdifferentiationinthe
chickenembryo. SupportedbyAustralianPostgraduateAward(APA) &CSIROPhDScholarship.
 I discoveredthe firstmicroRNA (miR202*) associatedwithtesticulardevelopmentin
vertebrate embryos.
 I published3firstauthorpapersdetailingsexually-dimorphicmicroRNA expression
dynamicsandthe regulationof miR202* duringtesticularvs.ovariandevelopment.
 I receivedthe AAHL, CSIRO studentseminarspeaker of the year award for 2009 for the
presentationof my work at the departmental seminarseries.
Contact(s):Dr. Mark Tizard [+61 3 5277 5753; Mark.Tizard@csiro.au] ;Ass.Prof. Dr.
Craig Smith[+61 3 9905 0203; craig.smith@monash.edu]
2006-2007 Honours Research Student (BSc. Biotech. Hons.)
Deakin University & CSIRO Australian Animal Health Laboratories (Australia)
Thesis:Characterisationof the chicken7SKsmall nuclearRNA promoterforthe expressionof
short hairpinRNAs.
 I characterizedthe activityof a new promoterforuse inexpressingshortRNAsthattrigger
the RNA interference pathway.
 Thistechnique canbe usedto inhibit the activityof specificgenes.Itestedthe relative
activityof the newlyidentifiedpromoter,againstexistingpromotersfordrivinggene
inhibition,andpublishedmyfindingsinBMCBiotechnologyin2007.
 This research was supportedby a Deakin UniversityHonoursScholarship.
Contact(s):Adj.Prof.Dr. Tim Doran [+61 3 5277 5788; Timothy.Doran@csiro.au] &
Prof.DavidCahill [+61 3 5337 1299; david.cahill@deakin.edu.au]
2005 - 2006 Vacation Research Scholarship
PeterMacCallum Cancer Centre (Australia)
 I optimizedprotocols tostudythe functionof cellularpolarityregulatorsin cancer
metastasis,usingRNA interference toinhibitthe activityof keygenesof interest.This
researchwassupportedby6-weekvacationscholarship.
 I was awarded the Nicole Lundie Summer StudentPrize for my work.
Contact(s):Dr. PatrickHumbert[+61 3 9479 5155; p.humbert@latrobe.edu.au] &Dr.
Lukas Dow [lud2005@med.cornell.edu]
Teaching
2011 - 2016 Research Student Supervision
Tessmar & Raible Groups,MFPL & The UniversityofVienna.
 Supervisionof 4 bachelorthesisprojects,3master practical projects,1 masterthesis.
 Molecularbiologyandgeneticstheoryandexperimental techniques,care and
maintenance of geneticlines formarine bristle worms.
 Coachingstudentstodevelopandimprove skillsin:time-management,literature review,
experimental design,scientificwriting,andscientificpresentations.
2013 - 2016 Lecturer & Course Instructor
Developmental NeurobiologyMasterPractical Course,The UniversityofVienna.

3.  Annual Lecture onprinciplesandtechnical applicationof genome editingtechniques
(TALEN & CRISPR/Cas9;2013-2016).
 Instructionof 2-weeklaboratorypractical course (2013) involving3differentmodel
organisms,cell culture,insituhybridization,microscopy,andPCR.
2009 Course Tutor
Undergraduate Ecology and Environment, Deakin University(Australia)
 1 hourtutorial classper fortnight for1 semester. Explanationof ecological theoryand
principles,andexperimental design; Supervisionof assignmentwork;Assignmentmarking.
2004 – 2005 Laboratory Demonstrator
High School biologypractical course, DeakinUniversity(Australia)
 RecombinantDNA technologies, DNA electrophoresisandquantitation, genetics
techniquesandtheory.
Organisational &Leadership Experience
 Lead researcher for collaborative project to develop custom behaviouraltracking
software (2015-2016) [Pattern Recognition and Image Processing group TU Vienna &
Tessmar Group, University of Vienna, Austria]. I tooka leadrole in establishingand
managingthisinterdisciplinarycollaboration whichinvolvedworkdone byabachelorand
masterstudentfromthe PRIPgroup.I contributedvideosforanalysisand advisedonanalysis
parametersthatthe software shoulddetect,communicatedfeedbackontestdataanalyses
and coordinatedmeetingswiththe projectteammembers[bachelorandmasterstudents,
advisingpostdoc,andtwoPrincipal Investigators]. Igainednew understandingof how
computational algorithmsworkandcan be developedtotrackmovingobjects,shape definition
and interpretationof animal behaviouraldata.The interdisciplinarynature of thiscollaboration
strengthenedmycommunicationskills.I learnedhow explainmyresearchquestionsand
articulate technical challengesina more generally-understandable way.
 Co-Founder of Postdoc Career Development Austria Project (2015 - current) [Vienna,
Austria]: I startedthis volunteerprojecttogetherwithacolleague,andthe supportof a
managementconsultant, toevaluateandrecommendimprovementsto provide improved
careerdevelopmentsupportforPostDocsworkinginAustria. Keyachievementsandgoals
include the organisationof the first Austria-wide postdocforum workshop, and a pilotcareer
coaching program. From these activities,togetherwith researchtoidentifybestpractices
nationallyandinternationally,the projectaimsto reportrecommendationsfor government,
fundingbodiesand institutes.We successfullysecuredfundingforthisproject, fromthe
Austrian Ministry of Science, Research and Economy (bmwfw), Ludwig Boltzmann Society (LBG),
and ViennaScience andTechnologyFund(WWTF)
 Girl Gone International Community Manager (2016) [GGI community, Vienna, Austria:
https://www.facebook.com/groups/GGIVienna/]. GGI is an international organizationfor
expatwomenwithhubsincitiesall overthe world. Icoordinate a team ofeventhosts to
organize networkingandsocial eventsforGGImembers,andmaintainonline social media
resourcesfor communityengagement(egfacebook,meetup).
 Seminar Host & Chairperson (2016) [Vienna BioCenter, Vienna Austria] Nominationand
Invitation of guestspeakersforcampusseminarseries, chairingspeakerseminars,coordination
of networkingopportunitiesforstudentsandpostdocs.
 PostDoc Representative (2014-2015) [MFPL, Vienna, Austria]: Organisationof institute
skillstrainingprogram,reportingtoscientificadvisoryboard andinstitutedirectorate,
advocacy forpostdocsupportneeds withinthe institute,organizationof careerdevelopment,
networking,andsocial events.

4.  Dragonboat Team Manager/Captain (2013-2015) [MFPL/University of Vienna/GMI
team, Vienna Austria] Organisationof teamtrainings,uniforms,sponsorship andtournament
registration;instructingtechnique,teambuilding&motivation.
 Head of Organising Committee for Annual PhD & PostDoc Retreat (2011-2013) [MFPL,
Vienna, Austria] Coordination andchairingof planningmeetings,assigningtasks,scheduling
and programplanning,chairingsymposiumsessions.
Professional Qualifications & Experience
 AcademicProof Reading(2016) [PRIDEProjectHandbook,Austria;ContactDr.Lucas Zinner:
lucas.zinner@univie.ac.at]
 Leadership&LaboratoryManagementcourse for Postdoctoral Researchers(2014, HFP
Consulting,IMP,Vienna)
 ProjectManagement Training(2012; MFPL, Vienna)
 Time ManagementTraining(2012; MFPL, Vienna)
 BusinessIPandManagementessentialscourse (2009-2010; CSIRO,Australia)
 Australian Standard annual accreditation for safe use of radioactive isotopes (2007-2010;
ARPANSA;CSIRO,Australia)
Professional Memberships
 AustrianAssociationof MolecularLife SciencesandBiotechnology (OegmbtAustria:2013-
2016)
 National PostdocAssociation(NPA,USA: 2012-2013)
 AustralianSocietyforDevelopmental Biology(ASDB,Australia:2010-2011)
 AustralianSocietyforBiochemistryandMolecularBiology (ASBMB,Australia:2007-2011)
Teaching
2011 - 2016 Research Student Supervision
Tessmar & Raible Groups,MFPL & The UniversityofVienna.
 Supervisionof 4 bachelorthesisprojects,3masterpractical projects,1 masterthesis.
 Molecularbiologyandgeneticstheoryandexperimental techniques,care and
maintenance of geneticlines formarine bristle worms.
 Coachingstudentstodevelopandimprove skillsin:time-management,literature
review,experimental design,scientificwriting,andscientificpresentations.
2013 - 2016 Lecturer & Course Instructor
Developmental NeurobiologyMasterPractical Course,The University ofVienna.
 Annual Lecture onprinciplesandtechnical applicationof genome editingtechniques
(TALEN & CRISPR/Cas9;2013-2016).
 Instructionof 2-weeklaboratorypractical course (2013) involving3differentmodel
organisms,cell culture,insituhybridization,microscopy,andPCR.
2009 Course Tutor
Undergraduate Ecology and Environment, Deakin University(Australia)
 1 hourtutorial classper fortnight for1 semester. Explanationof ecological theoryand
principles,andexperimental design; Supervisionof assignmentwork;Assignment
marking.
2004 – 2005 Laboratory Demonstrator
High School biologypractical course, DeakinUniversity(Australia)
 RecombinantDNA technologies,geneticstechniquesandtheory.

5. Peer ReviewedPublications
Bannister S, etal., (2014) TALENs mediate efficientandheritable mutationof endogenousgenesin
the marine annelid Platynereisdumerilii. Genetics.DOI:10.1534/genetics.113.161091. PMID
24653002.
Zantke J*, BannisterS*, etal., (2014) Geneticandgenomictoolsfor the marine annelid Platynereis
dumerilii. Genetics.DOI:10.1534/genetics.112.148254. PMID 24807110. *equal contributions
Lidke AK, BannisterS, et al., (2013) 17β-Estradiol inducessupernumeraryprimordial germcellsin
embryosof the polychaete Platynereisdumerilii.GenCompEndocrinol.
DOI:10.1016/j.ygcen.2013.11.017. PMID: 24287341.
CuttingAD, Bannister SC, etal., (2012) The potential role of microRNAsinregulatinggonadal sex
differentiationinthe chickenembryo. Chromosome Res.DIO:10.1007/s10577-011-9263-y. PubMed
PMID: 22161018.
Bannister SC,et al., (2011) Manipulationof estrogensynthesisaltersMIR202* expressionin
embryonicchickengonads. Biol Reprod.DOI:10.1095/biolreprod.110.088476. PubMedPMID:
21389341.
Bannister SC,et al., (2009) SexuallydimorphicmicroRNA expressionduring chickenembryonic
gonadal development. Biol Reprod.DOI:10.1095/biolreprod.108.074005. PMID: 19357368.
Bannister SC,et al., (2007) Comparisonof chicken7SKand U6 RNA polymeraseIIIpromoters for
short hairpinRNA expression. BMCBiotechnol.DOI:10.1186/1472-6750-7-79. PMID: 18021456.
Conference Presentations
Bannister S.C.et al.,(September2016) From DiscoverytoFunction:UsingTranscriptomics and
Proteomicstoinvestigatethe molecularbasisof lunar-reproductivetiminginPlatynereisdumerilii.
PolarBiology SummerSchool.Spiekeroog,Germany.Oral Presentation.
Bannister S.C.et al.,(May 2016) Illuminatingmolecularchangesincircalunar reproductive timing:
A transcriptomicsapproachin Platynereisdumerilii. Time and Light Symposium.Vienna,Austria.
Oral Presentation.
Bannister S.C.et al.,(September2015) Investigatingmolecularregulatorsof sexual dimorphismin
the marine polychaete Platynereisdumerilii.AnnualMeeting of theGerman ZoologicalSociety
(DZG).Graz, Austria. InvitedOral Presentation.
Bannister S.C. etal., (July2014) The newworm onthe block:usingTALENsto investigatespecific
biological phenomenainnon-conventional modelorganisms. FASEBConferenceon Genome
Engineering – Cutting edgeresearch and applications.Nassau,Bahamas. Oral Presentation.
Bannister S.C. etal., (November2013) Efficientprecisiongenomeengineeringinthe marine
polychaete PlatynereisdumeriliiusingTranscriptional ActivatorLike EffectorNucleases(TALENs).
InternationalMarineBiotechnology Congress,Brisbane,Australia. Oral Presentation.

6. Bannister S.C., et al.,(July2013) Investigatingthe geneticandhormonal regulationof sexual
differentiationandmaturationin Platynereisdumerilii.InternationalCongressof Invertebrate
Reproduction and Development,Detroit,Michigan,USA.PosterPresentation
Bannister S.C., et al.,(September2011) Assessingthe evolutionandfunctionof EstrogenReceptor
signalling in the marine annelid model Platynereis dumerilii. EMBO Nuclear Receptors, Sitges, Spain.
PosterPresentation.
Bannister S.C., SmithC.A.,BuermansH.,SinclairA.H.,DoranT.J., TizardM.L.V. (2010) Sexually-
dimorphicexpressionof MIR202* isassociatedwithtestisdifferentiationinthe male chicken
embryo. OzBio2010, Melbourne,Australia.Posterpresentation.
Bannister S.C., SmithC.A.,TizardM.L.V.,Doran T.J., SinclairA.H.(2010) Estradiol-mediateddown-
regulationof MIR202* expressioninchickembryonicgonads:Confirmingthe linkbetween
MIR202* and embryonictestisdevelopment. LorneGenome2010, Lorne,Australia.Poster
presentation.
Bannister S.C., BuermansH., Broz I., Tizard M.L.V.,Doran T.J.,SinclairA.H.,SmithC.A.(2009)
CharacterisingmicroRNA expressionandfunctionduringembryonicgonadal development. 16th
International Society for Developmental Biology Congress 2009, Edinburgh, UK. Poster presentation.
Bannister S.C., SmithC.A.,TizardM.L.V.,Doran T.J., SinclairA.H.(2009) Exploringthe functionof
miR-202* in embryonicgonadal sex differentiation. LorneGenome2009, Lorne,Australia.Poster
presentation.
Bannister S.C., SmithC.A.,TizardM.L.V.,Doran T.J., SinclairA.H.(2008) miR-202*: A potential role
for miRNAsinregulatingsexual differentiationof vertebrate embryonicgonads. ComBio 2008,
Canberra,Australia.Posterpresentation.
Bannister S.C., Tizard M.L.V.,SmithC.A.,DoranT.J., SinclairA.H.(2008) Characterisingthe miRNA
profilesof chickenembryonicgonadsduringsexual differentiation. LorneGenome2008, Lorne,
Australia.Posterpresentation.
Bannister S.C., Tizard M.L.V.,SmithC.A.,DoranT.J., SinclairA.H.(2007) MicroRNA expression
profilingof chickembryonicgonadsduringsex determination. ComBio 2007, Sydney,Australia.
Oral presentation.
Scientific andTechnical Skills
Transcriptome sequencing: RNA extraction (including small isolation), RNA QC, sample preparation
for Illuminasequencing,miRNA isolation,miRNA microarray.
Genome editing: Optimising TALEN & CRISPR/Cas9 reagents and protocols for targeted mutation
and insertion/editing in multiple species, donor design, establishing molecular mutation
screening assays, selective breeding of genetic mutant lines, standardising protocols and
trainingof researchstudentsandstaff intechniques.
Molecular Biology & Recombinant DNA techniques: PCR (normal and quantitative RT-PCR), cloning,
restriction endonuclease digest assays, southern, northern & western blotting, BAC
screening, expression plasmid and transgene design and construction, nucleic acid
extractionandseparationtechniques,proteinextractionformassspectrometryanalysis.
IT, bioinformatics & statistical analysis: Proficientin R and basic proficiency in Linux/Unix command
line. RNAseq data analysis: sequencing data QC, read filtering, read mapping, basic de novo
transcriptome assembly curation, differential expression analysis [SAMtools, NextGenMap,
IGV genome viewer, DESeq2, EdgeR]; data normalization for quantitative transcriptomic and
proteomic analyses; transcriptome/proteome annotation & gene ontology enrichment
[InterProScan, Blast2GO, NCBI blast, SMART, Swissprot/Uniprot, GOstats]. Highly proficient
in MS office programs [Word, Excel, Powerpoint]; Highly proficient in use of CLC workbench

7. genetic analysis software. Statistical analysis software - including omics data :R, GraphPad
Prism.Image analysissoftware:ZenPro(Zeiss),Adobe Illustrator,ImageJ.
in vivo gene expression analysis, embryo manipulation & cell culture techniques: Embryo
microinjection (chicken, marine bristle worm), ex vivo embryo culture (chicken), isolation
and culture of primary cells from marine bristle worm, chicken and mammalian cell culture,
transfection of adherent cells, FACS, virus isolation from cell culture media, viral
transduction, whole-mount in situ hybridization, immunohistochemistry (cryo-sections),
brightfieldmicroscopy,stereomicroscopy,fluorescence microscopy.
Professional Skills
Leadership& Project Management
 I have led2 researchprojectsduringmypostdocinvolvinginterdisciplinarycollaborations
and managedmyownresearchprojects,alongside thoseof several bachelorand
master/practical students.
 I chairedthe organizingcommitteeforannual institutePhD&PostdocRetreatfor 3 years,
managinga teamof 4-6 people.Iwasresponsibleforassemblingthe team, organisingthe
budget,assigningtasks,programscheduling,collectingfeedbackandreportingtothe
directorate.
 Co-communitymanagerforViennacommunityof Girl Gone International:A groupfor
international expatwomen(https://www.facebook.com/groups/GGIVienna/).Imanage
online facebookcommunityresourcesandorganize social andnetworkingeventswitha
teamof eventhosts.
Teamwork & Organisational skills
 Have workedon 3 projectswithexternal collaboratorsandseveral lab-internal projects
involvingsmallteamsof colleaguesonavarietyof researchtopics.
 As a PostdocRepresentative(2013-2015) I workedtogetherwithotherrepstodesign
transferable skillstrainingprogramsandtoorganize retreats,institutevisits,andseminar
series.
 Co-organisedthe firstPostDocForumAustria2016 andco-foundedaprojectto evaluate
careerDevelopmentsupportneedsforPostDocsinAustria(2015 – current)
 Memberof women’srugbyunion team(RUDonauWien,since June 2016), participatedin
institute Dragonboatteam(2011-2015), and previouslyplayedindoorvolleyball andfield
hockey.
Presentation,Communication& Networking
 Published4firstauthor papers,2 co-firstauthorpapers(including1review),andone second
author paperininternational peer-reviewedscientificjournals.Reviewer forBiologyof
Reproduction.
 Presentedtalksat5 international conferences,includingone invitedtalk. Presenteda
Science Slamforgeneral audience in2012: title “Whatmakeswormssexy”
(https://www.youtube.com/watch?v=CnhHETDoimE)
Mentoring,ResearchSupervision& Teaching
 Supervised4bachelorstudents,3master-level practical students,andcurrentlyone master
research student.
 I routinely mentorandtraincolleaguesin:molecularbiologyandgeneticstheory,
experimental techniques,dataanalysismethods,experimentaldesign,scientificwriting,and
scientificpresentationskills.
 Annual lecture ongenome editingtechniquesandapplicationsand taughtatwo-week
Master Practical Laboratory course on developmental neurobiology (May/June 2012).
Awards & Fellowships

8. 2014 FASEB Travel fellowship for Young Scientists
Grantedto enable attendance of the 2014 FASEBGenome Engineering
Conference.Amount$1000 USD.
2011-2014 VIPS postdoctoral fellowship: Max F. Perutz Laboratories.
Awarded by competitive selectionfrompool of over350 international
applicants.FundedbyThe AustrianMinistryforScience andThe Cityof
Vienna.Salaryfir3 years+ 14,000 euroresearch,travel andtrainingbudget
peryear.
2010 Young Scientists Forum Fellowship
OzBio2010 conference (Melbourne,Australia).Coveredcostsof attendingYSF
meeting,andregistrationandaccommodationforOzBioconference.
2009 Seminar of the Year 2009 Student Award
(CSIROLivestockIndustries,AAHL, Geelong).Certificate.
2009 Conference Participation Award
NetworkinGenesEnvironmentandDevelopment(NGED,Australia).Value of
500 AUD to assistwithcostsof overseasconference attendance.
2007 FABLS Travel Scholarship
LudwigScientificImagingWorkshop(FABLSNetwork,Australia).Coveredcost
of registrationforworkshopattendance.
2007 -2010 PhD Top-up Scholarship
(CSIROLivestockIndustries,AAHL,Geelong).3-yeartop-uptoPhDstipendto
bringtotal stipendto28,000.00 AUD/year
2007 -2010 Australian Postgraduate Award
(The Universityof Melbourne,Melbourne).BasedonNational Ranking,
provides3year PhDStipend19,696.00 AUD/year.
2006 Deakin Honours Scholarship
(DeakinUniversity,Geelong).Value:3,000.00 AUD.
2005 - 2006 Nicole Lundie Summer Student Research Award
(PeterMacCallumCancerCentre,Melbourne).Awarddonatedbythe Lions
Clubof Geelong,value:500.00 AUD.
2005 – 2006 Summer Vacation Studentship
(Cell Cycle andCancerGeneticsLaboratory,PeterMacCallumCancer Centre,
Melbourne).6-weekstipend,value approx 2,500.00 AUD.
2005 David Stokes Award
Awardedtograduatingstudentwithhighestmarksinthe Science and
TechnologyFaculty(DeakinUniversity,Geelong).
2005 Alfred Deakin Medal
Awardedforoutstandingacademicundergraduate performance (Deakin
University,Geelong).
Interests &Hobbies
 RugbyUnion DonauWienRugbyClubMembership:2016-current
 Advocacyfor improvementstocareerdevelopmentsupportandworkingconditionsfor
postdoctoral researchers.
 Facilitatingnetworkingopportunitiesforexpatandinternationalwomen.