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Nybogatan 17, 273 30 Tomelilla
Sweden
Tel: +46 (0)417 10373
Mobile: +46 (0)730 510373
Email: Mattias.Gustafsson@tomelilla.nu
CURRICULUM VITAE
Mattias Gustafsson
SUMMARY
A senior level Ph.D. with extensive knowledge in microbiology/infection and tumour biology through 14
years of postdoctoral experience, including three years of international experience. I have thus a very
broad experience of various lines of research and am technically versatile. Furthermore, I have
extensive experience in oral and written presentation as well as teaching and supervision.
I am often described as “an initiator and strategic thinker with the ability to interact within all levels
of an organization”. I’m an enthusiastic, knowledge-hungry self-starter, eager to meet challenges and
quickly assimilate the newest and latest technologies, skills, concepts, and ideas. I work well as a
team member, am very reliable, organized and willing to learn. I am also a creative problem solver
that works hard and loves a challenge.
I work proactively and enjoy teams that cross the boundaries of professions and areas of
specialization where team members can give their two cents, analysing and sometimes provoking in
good spirits to create new solutions and order from chaos.
I have had both leading and supportive roles in the various projects. I can thus both be the supportive
team player as well as the driving force in a project.
KEY PERSONALITIES
Responsibility taking Creative Team player
CAREER GOALS
I have asked various people for advice on what kind of career in the private sector that would suit my
skills profile and have been advised to look into pharmacovigilance, medical writing and medical
scientific liaison jobs. I have attended “Grundkurs i pharmacovigilance” given by The Swedish Academy
of Pharmaceutical Sciences (Läkemedelsakademin).
CURRICULUM VITAE Mattias Gustafsson
Page 2 of 11
PROFESSIONAL EXPERIENCE
Work for Professor Gunnar Lindahl
2012–2014 Postdoctoral Researcher Lund University
Responsibilities
 Research on group A streptococci (Streptococcus pyogenes) and
immune system interactions.
 Supervision of junior colleagues.
 Maintenance and genotyping of transgenic mice as well as other mouse
work.
 IT infrastructure (installing computers and software).
Achievements
 Successfully co-supervised a Ph.D. Student, Rickard Nilsson, (I was not
the official supervisor) that graduated from the University of
Copenhagen in 2014.
 Successfully provided ideas and contributed to a project concerning the
binding of human complement Factor H to streptococcal M protein. This
led to a first author article in the high-ranking journal PLOS Pathogens
(article 3 in the appendix).
 Successfully co-led a project where we invented and developed a
superior method for the purification of complement Factor H. This led
to the filing of a patent application and a last author article in PLOS
One (article 2).
 Successfully maintained two hemizygotic transgenic mouse strains used
for experiments published in PLOS Pathogens.
 Successfully contributed to a project published in the journal
MicrobiologyOpen (article 1).
2010–2012 Postdoctoral Researcher University of Copenhagen
Responsibilities
 Research on group A streptococci and their interactions with the host
immune system.
 Supervision of junior colleagues.
 Mouse work.
 IT infrastructure (installing computers and software).
Achievements
 I successfully contributed to and solved problems in a project were we
showed that the S. pyogenes M protein hypervariable region avoids
antibodies both by antigen variation and poor immunogenicity This was
published (shared first author) in the high-ranking journal Cell Host &
Microbe (article 5).
 I co-supervised a Ph.D. Student (not the official supervisor) and
generated ideas for his thesis project. This was very successful (please
see under “Achievements” above)
 When the colleague handling our mice fell ill, I took over the
responsibility for the mouse work during her convalescence.
2008–2010 Postdoctoral Researcher Lund University
Responsibilities
 Research on group A streptococci and their interactions with the host
immune system.
 Supervision of junior colleagues.
CURRICULUM VITAE Mattias Gustafsson
Page 3 of 11
 IT infrastructure (installing computers and software).
Achievements
 For scientific achievements published in international journals, please
see above.
 Radically cut the cost of software by contacting the university software
support.
 Introduced the Staden package to improve the existing means for
analysing DNA sequences.
Work for Professor Catharina Svanborg
2007–2008 Postdoctoral Researcher/Microbiologist Region Skåne
Responsibilities
 Research on HAMLET (human lactalbumin made lethal to tumor cells), a
protein that kills tumor cells.
 Research on urinary tract infections with Escherichia coli as model
bacterium and its interactions with the immune system.
 Supervision of junior colleagues.
 Teaching
Achievements
 I was the intellectual sparring partner in a successful project that was
published in the high-ranking journal Cancer Research (second author,
article 10).
 I successfully introduced the use of siRNA technology, RT-PCR, nucleic
acid transfection of cell lines, reporter gene technology and transgenic
cell lines in the laboratory.
 My contributions to various projects led to publications in PLOS One
(article 6 and 10), Journal of Infectious Diseases (article 11), Cellular
Microbiology (article 11) and International Journal of Cancer (article 9)
as well as reviews 1 and 2 in European Journal of Clinical Investigation
and Current Opinions in Microbiology, respectively.
 Successfully supervised two exam project students (not the official
supervisor).
 Planned and tutored in problem based learning (PBL) sessions at the
Biomedicine program at the Medical Faculty at Lund University.
 Tutored at PBL sessions on the courses General Pathology and
Homeostasis on the Medicine program at the Medical Faculty, Lund
University.
2006–2007 Senior Researcher HAMLET Pharma
Responsibilities
 Research on HAMLET.
 Research on urinary tract infections with E. coli as model bacterium
and its interactions with the host immune system.
 Supervision of junior colleagues.
 Teaching.
Achievements
 For scientific achievements, please see above.
 Tutored at PBL sessions on the course General Pathology on the
Medicine program at the Medical Faculty, Lund University.
CURRICULUM VITAE Mattias Gustafsson
Page 4 of 11
2004–2006 Postdoctoral Researcher Lund University
Responsibilities
 Research on urinary tract infections with E. coli as model bacterium
and its interactions with the host immune system.
 Supervision of junior colleagues.
 Teaching
 Oral presentations at conferences.
Achievements
 Successfully introduced a range of molecular biology techniques in
Professor Svanborg´s research group. This fundamentally changed the
planning of projects and what was perceived as technically feasible in
the laboratory.
 Wrote cases for and tutored at problem based learning (PBL) sessions
at the Biomedicine program at the Medical Faculty at Lund University.
 For scientific achievements, please see above.
 Held oral presentations at the 1st
National Meeting in Infectious
Biology, Linköping September 2005 and on Professor Svanborg´s behalf
at Mikrobiologiskt Vårmöte in Jönköping, May 2006, and at the 3rd
Finnish Microbial Pathogenesis day in Helsingfors, October 2005.
Work for Professor Colin Palmer
2001–2004 Postdoctoral Researcher University of Dundee
Responsibilities
 Research on Peroxisome Proliferator Activated Receptors, PPARs.
 Development of transgenic mice and cell lines for conditional
expression of PPARs.
 Management of a research assistant.
Achievements
 Successfully developed breast cancer cell lines for conditional
expression of human PPARδ. The findings were published in a second
author article in the high-ranking journal Cancer Research (article 15)
that has been cited 112 times.
 Successfully developed transgenic mice for conditional expression of
human PPARδ and a dominant negative derivative. This led to the
development of a model for psoriasis published in PLOS One (article 7)
and an article concerning genetic regulation and physiology in PPAR
Research (article 4)
 Successfully led a project in which I managed a research assistant. This
led to a first author publication in PLOS One (article 8).
 Was called upon to help with expertise with PPARs for a group at
Glasgow University which led to an article in Biochemica et Biophysica
Acta (article 14) that has been cited 57 times.
Part-time teacher at the Department of Microbiology, Lund University
2000–2000 Teacher Lund University
Responsibilities
 Teaching at the laboratory practicals at the advanced courses in
Microbiology and Prokaryotic genetics.
Achievements
 Successfully performed the assigned tasks.
CURRICULUM VITAE Mattias Gustafsson
Page 5 of 11
Work for Professor Claes von Wachenfeldt
1995–2000 Ph. D. student Lund University
Responsibilities
 Research on cytochromes P450 in Bacillus subtilis.
 Teaching
 Maintenance of some laboratory equipment.
Achievements
 I wrote and successfully defended my PhD thesis with the title ”
Bacterial Cytochromes P450: Studies on cytochrome P450 102A2 and
P450 102A3 of Bacillus subtilis.
 In a successful collaboration with Professor Sonenshein´s group at
Tufts University, Boston, we discovered a novel antibiotic resistance
mechanism that involved discovering a novel type of branched-chain
amino acid transporter in B. subtilis (AzlCD). These findings were
published in a second author article in Journal of Bacteriology (article
19 in the appendix) that has been cited 55 times.
 As part of collaboration I worked at the University of Dundee with
Professor Colin Palmer and Professor Roland Wolf for a few months.
This work was successful and led to a first author article in Archives of
Microbiology (article 18) and review 3 in Biochemical Society
Transactions. I was offered a postdoc position and later went to work
for Professor Palmer.
 I initiated and led a project where I discovered a substance excreted
by wild-type B. subtilis that can overcome the expression of a range of
genes in bacteria mutated in the gene for central transcriptional
regulator Spo0A. The findings were published in a first author article in
FEMS Microbiology Letters (article 17).
 I met Professor Andrew Munro at a conference and initiated a
collaboration with him and Professor Stephen Chapman. I worked in
their laboratory at the University of Edinburgh for a few months. The
collaboration involved studying the enzymology of two cytochromes
P450 from B. subtilis. I suggested the use of branched-chain fatty acids
endogenous to B. subtilis as substrates for the enzymes. The findings
of this project were published in a first author article in Biochemistry
(article 16) that has been cited 69 times. I was offered a postdoc
position with Professor Munro.
 Successfully supervised a project student.
 Taught on laboratory practicals on the courses given at the Department
of Microbiology.
Undergraduate work for Professor Mats Hansson and Professor Lars Hederstedt
1994 Undergraduate student Lund University
Responsibilities
 Research on haem biosynthesis in B. subtilis.
Achievements
 By serendipity and keen observation I solved my tasks and thus
contributed to the project. I developed a B. subtilis over-expression
system for the enzyme HemY and was the one who found that HemY is
a membrane protein. The findings were published in a second author
article in Biochemica et Biophysica Acta (article 20).
 Wrote and defended my undergraduate thesis.
CURRICULUM VITAE Mattias Gustafsson
Page 6 of 11
EDUCATION
2000 Doctor of Philosophy, Department of Microbiology, Lund University with
Professor Claes von Wachenfeldt as supervisor.
Key focuses: Molecular biology and biochemistry of cytochromes P450 in
the Gram positive model bacterium Bacillus subtilis.
1995 Bachelor of Science, Lund University, specializing in Microbiology.
Key focuses: In my exam work I studied the biochemistry of the haem
synthesis in the bacterium B. subtilis.
Courses: General chemistry, organic chemistry, biochemistry, genetics,
prokaryotic and eukaryotic molecular genetics, immunology, physiology,
microbiology, geology, ecology, mathematics, geology, plant physiology,
ecology, cell biology, membrane biochemistry, human physiology, vertebrate
histology and morphology.
TRAINING
2015 Grundkurs i Parmacovigilance, Läkemedelsakademin (Swedish Academy of
Pharmaceutical Sciences).
2011 Researcher’s program, a course in commercializing research, Medeon,
Malmö.
2010 Laboratory animal science, Medical Faculty, Lund University.
2005 Problem based learning course, Medical Faculty, Lund University.
2005 University teaching course “Högskolepedagogisk grundkurs”, Medical
Faculty, Lund University.
2001 Animal course, University of Aberdeen, U.K. I held a personal license for
working with experimental animals.
LANGUAGES
Swedish Native tounge
English Fluent
PERSONAL DATA
Date of birth: 22nd
of September 1970
Marital status: Married to Camilla (44)
Children: Hanna (15)
CURRICULUM VITAE Mattias Gustafsson
Page 7 of 11
Interests: Spending time with family and friends.
Outdoor activities, field biology environmental issues. I’m on the board of
the local chapter of Swedish Society for Nature Conservation
(Naturskyddsföreningen).
I’m training judo and Brazilian jiu jitsu two to four times a week to keep in
shape.
CURRICULUM VITAE Mattias Gustafsson
Page 8 of 11
APPENDIX
Skills profile
IN VIVO TECHNIQUES
 Responsible for breeding transgenic
mice
 Marking/biopsy of mice for genotyping
 Designed PCR-based genotyping assay
for transgenic mice
 Dissecting mice
 Isolated RNA from various tissues
 Bleeding mice, both by heart
punctuation and by cutting neck
arteries
 Intraperitoneal injection of mice
 For work regarding molecular biology
techniques regarding mice, please see
above
 Assisted in immunization of mice
 Assisted in infection of mice
IN VITRO TECHNIQUES
DNA and RNA techniques
 PCR
 Cloning
 Restriction enzyme cleavage analysis
 Preparation of chromosomal DNA from
mammalian cells, Bacillus subtilis,
Streptococcus pyogenes and
Escherichia coli
 Preparation of plasmid DNA with
various methods
 Transformation of B. subtilis, E. coli
and S. pyogenes
 Transfection of mammalian cells
 Southern blot
 Generation of high frequency of
recombination (Hfr) strains of E. coli
 Mapping of genetic markers on the E.
coli chromosome through mating with
a Hfr-strain
 Mapping of genetic markers of the B.
subtilis chromosome through three-
factor crossings
 Mapping of genetic marker on the B.
subtilis chromosome using a YAC
library
 Fractionation of restriction enzyme
cleaved chromosomal DNA by
ultracentrifugation
 DNA library-construction
 DNA library screening
 Generation of insertion and insertion-
deletion B. subtilis and S. pyogenes as
well as unmarked mutants in B. subtilis
 Transposon mutagenesis
 Site-directed mutagenesis
 Chemical mutagenesis
 Generation of random deletions using
exonuclease III and mung bean
nuclease
 Sequencing and publishing a DNA
sequence (GenBank accession no.
Y11043)
 Generating DNA constructs for non-
conditional gene-expression in B.
subtilis, E. coli, mammalian cell-lines
and transgenic mice
 Generating DNA constructs for
conditional gene-expression in B.
subtilis, E. coli, mammalian cell-lines
and transgenic mice
 RNA extraction from B. subtilis, E. coli
and mammalian cells
 Generation of cDNA from mammalian
RNA
 Northern blot
 Real time PCR analysis of mammalian
gene expression using Taqman and
SYBR green chemistry
Protein techniques
 T7 protein over-production systems
 Disruption of cells using a sonicator
 Disruption of cells using a French press
 Membrane and cytoplasmic fraction
preparations
 Phase fractionation of membrane
proteins using Triton X114
 Purification of histidine-tagged
proteins using Ni-agarose columns
 Purification of GST-tagged proteins
using glutathione-sepharose columns
 Purification of proteins using ion
exchange, gel filtration and ADP-
sepharose columns
 SDS-PAGE
 Western blot
 Determination of protein
concentrations using various methods
CURRICULUM VITAE Mattias Gustafsson
Page 9 of 11
 Determination of cytochrome P450
concentration by spectrophotometry
 Immunocytochemistry
 Determination of Kd of Cytochrome
P450s for ligands by
spectrophotometry
 Determination Ki of the transcriptional
repressor FatR for various ligands by
EMSA
 Determination of Kd for FatR using a
direct binding assay with a fluorescent
fatty acid
 Investigating enzyme reactions using
spectrophotometric and
spectrofluorometric methods
 ELISA
 Solid phase RIA
 Bacterial ligand and antibody binding
assays
Functional screening assays
 Bacterial two-hybrid assays
 Investigating PPAR agonist induced
transcriptional transactivation using
mammalian one-hybrid systems
 Using mammalian two-hybrid systems
to investigate PPAR/RXR
heterodimerisation and transactivation
 Investigating PPAR repression and
agonist induced activation
Bioinformatics
 Using the GCG and Staden packages for
handling smaller sequencing projects
 Using the GCG package for predicting open
reading frames
 Experience with the GCG, MacVector,
Vector NTI and Emboss packages
 Using BLAST for finding proteins similar to
unknown open reading frames
 Using the GCG package for predicting
transcriptional terminator structures
 In silico design of plasmids
 Generating phylogenetic trees using the
GCG program package
 Predicting transmembrane helices using the
GCG package
 Using RasMol for looking at 3D structures of
proteins
Other techniques in microbiology
 Gram staining
 Typing of bacteria using biochemical and
morphological criteria
 Determining generation times of bacteria
 One step growth curves of phage T4 of E.
coli
 Titration of bacteriophages
 Investigating antibiotic resistance of
bacteria
Microscopy
 General light microscopy
 Fluorescence microscopy
 Phase contrast microscopy
TEACHING
 Taught in the laboratory practicals of
the following courses given in the
biology program at Lund University
(three times for each course): five and
ten credits Microbiology and ten
credits procaryotic molecular genetics.
 Organized a week of the Molecular
Medicine course of the Biomedicine
program at Lund University for two
years.
 Tutored and wrote problem based
learning cases for one-two weeks on
the Molecular Medicine course of the
Biomedicine program at Lund
University (four times).
 Tutored for four weeks on the General
Pathology course of the Medicine
program, Lund University)
 Tutored for a half semester on the
Homeostasis course of the Medicine
program, Lund University.
 Tutored for two weeks on the
Pathobiology and Pharmacology course
on the Biomedicine program, Lund
University.
CURRICULUM VITAE Mattias Gustafsson
Page 10 of 11
MANAGEMENT/SUPERVISION
 As a Ph. D. student I supervised a
project student.
 As a postdoc in Dundee I led and
planned the work of a research
assistant.
 One of my main tasks in Professor
Svanborg´s laboratory was to
supervise members of the laboratory in
molecular biology techniques.
 During my appointment as postdoc
with Professor Svanborg’s group I did
to some extent supervise a Ph.D.
student (I was not a formal
supervisor). I also supervised two half
semester project students (not a
formal supervisor).
 In my current appointment I have to a
large extent supervised a Ph. D.
student at Copenhagen University with
Professor Lindahl and Prof. John Olsen
as the formal supervisors.
IT SYSTEMS
 Responsible for installing computers
and programs in our research group.
 Been working with Microsoft Windows
(XP, Vista and 7), Mac OS X Mountain
Lion.
 Microsoft Office (Word, Excel, Power
Point).
 Experience with the GCG and Emboss
packages.
 Experience with the Staden package.
 In silico cloning with MacVector and
VectorNTI
SCIENTIFIC PUBLICATIONS
Articles
1. Lannergård, J., Kristenssen, B., Gustafsson, M., Persson, J., Norrby-Teglund, A., Stålhammar-
Carlemalm, M. and Lindahl, G. (2015) Sequence variability is correlated to weak
immunogenicity in Streptococcus pyogenes M protein, MicrobiologyOpen Epub ahead of print
2. Nilsson, O. R., Lannergård, J., Morgan, B. P., Lindahl, G. and Gustafsson, M. C. (2013)
Affinity purification of human factor H on polypeptides derived from streptococcal M protein:
enrichment of the Y402 variant, PLoS One 8, e81303.
3. Gustafsson, M. C., Lannergård, J., Nilsson, O. R., Kristensen, B. M., Olsen, J. E., Harris, C. L.,
Ufret-Vincenty, R. L., Stålhammar-Carlemalm, M., and Lindahl, G. (2013) Factor H binds to
the hypervariable region of many Streptococcus pyogenes M proteins but does not promote
phagocytosis resistance or acute virulence, PLoS Pathogens 9, e1003323.
4. Higgins, L. G., Garbacz, W. G., Gustafsson, M. C., Nainamalai, S., Ashby, P. R., Wolf, C. R.,
and Palmer, C. N. (2012) Conditional expression of human PPARδ and a dominant negative
variant of hPPARδ in vivo, PPAR Research 2012, 216817.
5. Lannergård, J., *Gustafsson, M. C., Waldemarsson, J., Norrby-Teglund, A., Stålhammar-
Carlemalm, M., and Lindahl, G. (2011) The hypervariable region of Streptococcus pyogenes M
protein escapes antibody attack by antigenic variation and weak immunogenicity, Cell Host &
Microbe 10, 147-157.
* Please note that I´m shared first author this article.
6. Ragnarsdóttir, B., Jönsson, K., Urbano, A., Grönberg-Hernandez, J., Lutay, N., Tammi, M.,
Gustafsson, M., Lundstedt, A. C., Leijonhufvud, I., Karpman, D., Wullt, B., Truedsson, L.,
Jodal, U., Andersson, B., and Svanborg, C. (2010) Toll-like receptor 4 promoter
polymorphisms: common TLR4 variants may protect against severe urinary tract infection,
PLoS One 5, e10734.
7. Romanowska, M., Reilly, L., Palmer, C. N., Gustafsson, M. C., and Foerster, J. (2010)
Activation of PPARβ/δ causes a psoriasis-like skin disease in vivo, PLoS One 5, e9701.
8. Gustafsson, M. C., Knight, D., and Palmer, C. N. (2009) Ligand modulated antagonism of
PPARγ by genomic and non-genomic actions of PPARδ, PLoS One 4, e7046.
9. Aits, S., Gustafsson, L., Hallgren, O., Brest, P., Gustafsson, M., Trulsson, M., Mossberg, A. K.,
Simon, H. U., Mograbi, B., and Svanborg, C. (2009) HAMLET (human alpha-lactalbumin made
lethal to tumor cells) triggers autophagic tumor cell death, International Journal of Cancer
124, 1008-1019.
CURRICULUM VITAE Mattias Gustafsson
Page 11 of 11
10. Brest, P., Gustafsson, M., Mossberg, A. K., Gustafsson, L., Düringer, C., Hamiche, A., and
Svanborg, C. (2007) Histone deacetylase inhibitors promote the tumoricidal effect of HAMLET,
Cancer Research 67, 11327-11334.
11. Lundstedt, A. C., McCarthy, S., Gustafsson, M. C., Godaly, G., Jodal, U., Karpman, D.,
Leijonhufvud, I., Lindén, C., Martinell, J., Ragnarsdóttir, B., Samuelsson, M., Truedsson, L.,
Andersson, B., and Svanborg, C. (2007) A genetic basis of susceptibility to acute
pyelonephritis, PLoS One 2, e825.
12. Ragnarsdóttir, B., Samuelsson, M., Gustafsson, M. C., Leijonhufvud, I., Karpman, D., and
Svanborg, C. (2007) Reduced toll-like receptor 4 expression in children with asymptomatic
bacteriuria, Journal of Infectious Diseases 196, 475-484.
13. Fischer, H., Ellström, P., Ekström, K., Gustafsson, L., Gustafsson, M., and Svanborg, C.
(2007) Ceramide as a TLR4 agonist; a putative signalling intermediate between sphingolipid
receptors for microbial ligands and TLR4, Cellular Microbiology 9, 1239-1251.
14. Targett-Adams, P., McElwee, M. J., Ehrenborg, E., Gustafsson, M. C., Palmer, C. N., and
McLauchlan, J. (2005) A PPAR response element regulates transcription of the gene for
human adipose differentiation-related protein, Biochimica et Biophysica Acta-Gene Structure
and Expression 1728, 95-104.
15. Stephen, R. L., Gustafsson, M. C., Jarvis, M., Tatoud, R., Marshall, B. R., Knight, D.,
Ehrenborg, E., Harris, A. L., Wolf, C. R., and Palmer, C. N. (2004) Activation of peroxisome
proliferator-activated receptor δ stimulates the proliferation of human breast and prostate
cancer cell lines, Cancer Research 64, 3162-3170.
16. Gustafsson, M. C., Roitel, O., Marshall, K. R., Noble, M. A., Chapman, S. K., Pessegueiro, A.,
Fulco, A. J., Cheesman, M. R., von Wachenfeldt, C., and Munro, A. W. (2004) Expression,
purification, and characterization of Bacillus subtilis cytochromes P450 CYP102A2 and
CYP102A3: flavocytochrome homologues of P450 BM3 from Bacillus megaterium, Biochemistry
43, 5474-5487.
17. Gustafsson, M. C., and von Wachenfeldt, C. (2001) A novel diffusible substance can overcome
the apparent AbrB repression of the Bacillus subtilis fatR promoter, FEMS Microbiology Letters
199, 197-202.
18. Gustafsson, M. C., Palmer, C. N., Wolf, C. R., and von Wachenfeldt, C. (2001) Fatty-acid-
displaced transcriptional repressor, a conserved regulator of cytochrome P450 102
transcription in Bacillus species, Archives of Microbiology 176, 459-464.
19. Belitsky, B. R., Gustafsson, M. C., Sonenshein, A. L., and von Wachenfeldt, C. (1997) An lrp-
like gene of Bacillus subtilis involved in branched-chain amino acid transport, Journal of
Bacteriology 179, 5448-5457.
20. Hansson, M., Gustafsson, M. C., Kannangara, C. G., and Hederstedt, L. (1997) Isolated
Bacillus subtilis HemY has coproporphyrinogen III to coproporphyrin III oxidase activity,
Biochimica et Biophysica Acta-Protein Structure and Molecular Enzymology 1340, 97-104.
Reviews
1. Ragnarsdóttir, B., Fischer, H., Godaly, G., Grönberg-Hernandez, J., Gustafsson, M., Karpman,
D., Lundstedt, A. C., Lutay, N., Rämisch, S., Svensson, M. L., Wullt, B., Yadav, M., and
Svanborg, C. (2008) TLR- and CXCR1-dependent innate immunity: insights into the genetics
of urinary tract infections, European Journal of Clinical Investigation 38 Suppl 2, 12-20.
2. Svanborg, C., Bergsten, G., Fischer, H., Godaly, G., Gustafsson, M., Karpman, D., Lundstedt,
A. C., Ragnarsdóttir, B., Svensson, M., and Wullt, B. (2006) Uropathogenic Escherichia coli as
a model of host-parasite interaction, Current Opinion in Microbiology 9, 33-39.
3. Palmer, C. N., Gustafsson, M. C., Dobson, H., von Wachenfeldt, C., and Wolf, C. R. (1999)
Adaptive responses to fatty acids are mediated by the regulated expression of cytochromes
P450, Biochemical Society Transactions 27, 374-378.
Ph.D. thesis
1. Gustafsson, M. C. U. (2000) Bacterial cytochromes P450: Studies on cytochromes P450 102A2
and P450 102A3 of Bacillus subtilis. Department of Microbiology, Lund University, Lund,
Sweden.

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CVMGustafsson

  • 1. Nybogatan 17, 273 30 Tomelilla Sweden Tel: +46 (0)417 10373 Mobile: +46 (0)730 510373 Email: Mattias.Gustafsson@tomelilla.nu CURRICULUM VITAE Mattias Gustafsson SUMMARY A senior level Ph.D. with extensive knowledge in microbiology/infection and tumour biology through 14 years of postdoctoral experience, including three years of international experience. I have thus a very broad experience of various lines of research and am technically versatile. Furthermore, I have extensive experience in oral and written presentation as well as teaching and supervision. I am often described as “an initiator and strategic thinker with the ability to interact within all levels of an organization”. I’m an enthusiastic, knowledge-hungry self-starter, eager to meet challenges and quickly assimilate the newest and latest technologies, skills, concepts, and ideas. I work well as a team member, am very reliable, organized and willing to learn. I am also a creative problem solver that works hard and loves a challenge. I work proactively and enjoy teams that cross the boundaries of professions and areas of specialization where team members can give their two cents, analysing and sometimes provoking in good spirits to create new solutions and order from chaos. I have had both leading and supportive roles in the various projects. I can thus both be the supportive team player as well as the driving force in a project. KEY PERSONALITIES Responsibility taking Creative Team player CAREER GOALS I have asked various people for advice on what kind of career in the private sector that would suit my skills profile and have been advised to look into pharmacovigilance, medical writing and medical scientific liaison jobs. I have attended “Grundkurs i pharmacovigilance” given by The Swedish Academy of Pharmaceutical Sciences (Läkemedelsakademin).
  • 2. CURRICULUM VITAE Mattias Gustafsson Page 2 of 11 PROFESSIONAL EXPERIENCE Work for Professor Gunnar Lindahl 2012–2014 Postdoctoral Researcher Lund University Responsibilities  Research on group A streptococci (Streptococcus pyogenes) and immune system interactions.  Supervision of junior colleagues.  Maintenance and genotyping of transgenic mice as well as other mouse work.  IT infrastructure (installing computers and software). Achievements  Successfully co-supervised a Ph.D. Student, Rickard Nilsson, (I was not the official supervisor) that graduated from the University of Copenhagen in 2014.  Successfully provided ideas and contributed to a project concerning the binding of human complement Factor H to streptococcal M protein. This led to a first author article in the high-ranking journal PLOS Pathogens (article 3 in the appendix).  Successfully co-led a project where we invented and developed a superior method for the purification of complement Factor H. This led to the filing of a patent application and a last author article in PLOS One (article 2).  Successfully maintained two hemizygotic transgenic mouse strains used for experiments published in PLOS Pathogens.  Successfully contributed to a project published in the journal MicrobiologyOpen (article 1). 2010–2012 Postdoctoral Researcher University of Copenhagen Responsibilities  Research on group A streptococci and their interactions with the host immune system.  Supervision of junior colleagues.  Mouse work.  IT infrastructure (installing computers and software). Achievements  I successfully contributed to and solved problems in a project were we showed that the S. pyogenes M protein hypervariable region avoids antibodies both by antigen variation and poor immunogenicity This was published (shared first author) in the high-ranking journal Cell Host & Microbe (article 5).  I co-supervised a Ph.D. Student (not the official supervisor) and generated ideas for his thesis project. This was very successful (please see under “Achievements” above)  When the colleague handling our mice fell ill, I took over the responsibility for the mouse work during her convalescence. 2008–2010 Postdoctoral Researcher Lund University Responsibilities  Research on group A streptococci and their interactions with the host immune system.  Supervision of junior colleagues.
  • 3. CURRICULUM VITAE Mattias Gustafsson Page 3 of 11  IT infrastructure (installing computers and software). Achievements  For scientific achievements published in international journals, please see above.  Radically cut the cost of software by contacting the university software support.  Introduced the Staden package to improve the existing means for analysing DNA sequences. Work for Professor Catharina Svanborg 2007–2008 Postdoctoral Researcher/Microbiologist Region Skåne Responsibilities  Research on HAMLET (human lactalbumin made lethal to tumor cells), a protein that kills tumor cells.  Research on urinary tract infections with Escherichia coli as model bacterium and its interactions with the immune system.  Supervision of junior colleagues.  Teaching Achievements  I was the intellectual sparring partner in a successful project that was published in the high-ranking journal Cancer Research (second author, article 10).  I successfully introduced the use of siRNA technology, RT-PCR, nucleic acid transfection of cell lines, reporter gene technology and transgenic cell lines in the laboratory.  My contributions to various projects led to publications in PLOS One (article 6 and 10), Journal of Infectious Diseases (article 11), Cellular Microbiology (article 11) and International Journal of Cancer (article 9) as well as reviews 1 and 2 in European Journal of Clinical Investigation and Current Opinions in Microbiology, respectively.  Successfully supervised two exam project students (not the official supervisor).  Planned and tutored in problem based learning (PBL) sessions at the Biomedicine program at the Medical Faculty at Lund University.  Tutored at PBL sessions on the courses General Pathology and Homeostasis on the Medicine program at the Medical Faculty, Lund University. 2006–2007 Senior Researcher HAMLET Pharma Responsibilities  Research on HAMLET.  Research on urinary tract infections with E. coli as model bacterium and its interactions with the host immune system.  Supervision of junior colleagues.  Teaching. Achievements  For scientific achievements, please see above.  Tutored at PBL sessions on the course General Pathology on the Medicine program at the Medical Faculty, Lund University.
  • 4. CURRICULUM VITAE Mattias Gustafsson Page 4 of 11 2004–2006 Postdoctoral Researcher Lund University Responsibilities  Research on urinary tract infections with E. coli as model bacterium and its interactions with the host immune system.  Supervision of junior colleagues.  Teaching  Oral presentations at conferences. Achievements  Successfully introduced a range of molecular biology techniques in Professor Svanborg´s research group. This fundamentally changed the planning of projects and what was perceived as technically feasible in the laboratory.  Wrote cases for and tutored at problem based learning (PBL) sessions at the Biomedicine program at the Medical Faculty at Lund University.  For scientific achievements, please see above.  Held oral presentations at the 1st National Meeting in Infectious Biology, Linköping September 2005 and on Professor Svanborg´s behalf at Mikrobiologiskt Vårmöte in Jönköping, May 2006, and at the 3rd Finnish Microbial Pathogenesis day in Helsingfors, October 2005. Work for Professor Colin Palmer 2001–2004 Postdoctoral Researcher University of Dundee Responsibilities  Research on Peroxisome Proliferator Activated Receptors, PPARs.  Development of transgenic mice and cell lines for conditional expression of PPARs.  Management of a research assistant. Achievements  Successfully developed breast cancer cell lines for conditional expression of human PPARδ. The findings were published in a second author article in the high-ranking journal Cancer Research (article 15) that has been cited 112 times.  Successfully developed transgenic mice for conditional expression of human PPARδ and a dominant negative derivative. This led to the development of a model for psoriasis published in PLOS One (article 7) and an article concerning genetic regulation and physiology in PPAR Research (article 4)  Successfully led a project in which I managed a research assistant. This led to a first author publication in PLOS One (article 8).  Was called upon to help with expertise with PPARs for a group at Glasgow University which led to an article in Biochemica et Biophysica Acta (article 14) that has been cited 57 times. Part-time teacher at the Department of Microbiology, Lund University 2000–2000 Teacher Lund University Responsibilities  Teaching at the laboratory practicals at the advanced courses in Microbiology and Prokaryotic genetics. Achievements  Successfully performed the assigned tasks.
  • 5. CURRICULUM VITAE Mattias Gustafsson Page 5 of 11 Work for Professor Claes von Wachenfeldt 1995–2000 Ph. D. student Lund University Responsibilities  Research on cytochromes P450 in Bacillus subtilis.  Teaching  Maintenance of some laboratory equipment. Achievements  I wrote and successfully defended my PhD thesis with the title ” Bacterial Cytochromes P450: Studies on cytochrome P450 102A2 and P450 102A3 of Bacillus subtilis.  In a successful collaboration with Professor Sonenshein´s group at Tufts University, Boston, we discovered a novel antibiotic resistance mechanism that involved discovering a novel type of branched-chain amino acid transporter in B. subtilis (AzlCD). These findings were published in a second author article in Journal of Bacteriology (article 19 in the appendix) that has been cited 55 times.  As part of collaboration I worked at the University of Dundee with Professor Colin Palmer and Professor Roland Wolf for a few months. This work was successful and led to a first author article in Archives of Microbiology (article 18) and review 3 in Biochemical Society Transactions. I was offered a postdoc position and later went to work for Professor Palmer.  I initiated and led a project where I discovered a substance excreted by wild-type B. subtilis that can overcome the expression of a range of genes in bacteria mutated in the gene for central transcriptional regulator Spo0A. The findings were published in a first author article in FEMS Microbiology Letters (article 17).  I met Professor Andrew Munro at a conference and initiated a collaboration with him and Professor Stephen Chapman. I worked in their laboratory at the University of Edinburgh for a few months. The collaboration involved studying the enzymology of two cytochromes P450 from B. subtilis. I suggested the use of branched-chain fatty acids endogenous to B. subtilis as substrates for the enzymes. The findings of this project were published in a first author article in Biochemistry (article 16) that has been cited 69 times. I was offered a postdoc position with Professor Munro.  Successfully supervised a project student.  Taught on laboratory practicals on the courses given at the Department of Microbiology. Undergraduate work for Professor Mats Hansson and Professor Lars Hederstedt 1994 Undergraduate student Lund University Responsibilities  Research on haem biosynthesis in B. subtilis. Achievements  By serendipity and keen observation I solved my tasks and thus contributed to the project. I developed a B. subtilis over-expression system for the enzyme HemY and was the one who found that HemY is a membrane protein. The findings were published in a second author article in Biochemica et Biophysica Acta (article 20).  Wrote and defended my undergraduate thesis.
  • 6. CURRICULUM VITAE Mattias Gustafsson Page 6 of 11 EDUCATION 2000 Doctor of Philosophy, Department of Microbiology, Lund University with Professor Claes von Wachenfeldt as supervisor. Key focuses: Molecular biology and biochemistry of cytochromes P450 in the Gram positive model bacterium Bacillus subtilis. 1995 Bachelor of Science, Lund University, specializing in Microbiology. Key focuses: In my exam work I studied the biochemistry of the haem synthesis in the bacterium B. subtilis. Courses: General chemistry, organic chemistry, biochemistry, genetics, prokaryotic and eukaryotic molecular genetics, immunology, physiology, microbiology, geology, ecology, mathematics, geology, plant physiology, ecology, cell biology, membrane biochemistry, human physiology, vertebrate histology and morphology. TRAINING 2015 Grundkurs i Parmacovigilance, Läkemedelsakademin (Swedish Academy of Pharmaceutical Sciences). 2011 Researcher’s program, a course in commercializing research, Medeon, Malmö. 2010 Laboratory animal science, Medical Faculty, Lund University. 2005 Problem based learning course, Medical Faculty, Lund University. 2005 University teaching course “Högskolepedagogisk grundkurs”, Medical Faculty, Lund University. 2001 Animal course, University of Aberdeen, U.K. I held a personal license for working with experimental animals. LANGUAGES Swedish Native tounge English Fluent PERSONAL DATA Date of birth: 22nd of September 1970 Marital status: Married to Camilla (44) Children: Hanna (15)
  • 7. CURRICULUM VITAE Mattias Gustafsson Page 7 of 11 Interests: Spending time with family and friends. Outdoor activities, field biology environmental issues. I’m on the board of the local chapter of Swedish Society for Nature Conservation (Naturskyddsföreningen). I’m training judo and Brazilian jiu jitsu two to four times a week to keep in shape.
  • 8. CURRICULUM VITAE Mattias Gustafsson Page 8 of 11 APPENDIX Skills profile IN VIVO TECHNIQUES  Responsible for breeding transgenic mice  Marking/biopsy of mice for genotyping  Designed PCR-based genotyping assay for transgenic mice  Dissecting mice  Isolated RNA from various tissues  Bleeding mice, both by heart punctuation and by cutting neck arteries  Intraperitoneal injection of mice  For work regarding molecular biology techniques regarding mice, please see above  Assisted in immunization of mice  Assisted in infection of mice IN VITRO TECHNIQUES DNA and RNA techniques  PCR  Cloning  Restriction enzyme cleavage analysis  Preparation of chromosomal DNA from mammalian cells, Bacillus subtilis, Streptococcus pyogenes and Escherichia coli  Preparation of plasmid DNA with various methods  Transformation of B. subtilis, E. coli and S. pyogenes  Transfection of mammalian cells  Southern blot  Generation of high frequency of recombination (Hfr) strains of E. coli  Mapping of genetic markers on the E. coli chromosome through mating with a Hfr-strain  Mapping of genetic markers of the B. subtilis chromosome through three- factor crossings  Mapping of genetic marker on the B. subtilis chromosome using a YAC library  Fractionation of restriction enzyme cleaved chromosomal DNA by ultracentrifugation  DNA library-construction  DNA library screening  Generation of insertion and insertion- deletion B. subtilis and S. pyogenes as well as unmarked mutants in B. subtilis  Transposon mutagenesis  Site-directed mutagenesis  Chemical mutagenesis  Generation of random deletions using exonuclease III and mung bean nuclease  Sequencing and publishing a DNA sequence (GenBank accession no. Y11043)  Generating DNA constructs for non- conditional gene-expression in B. subtilis, E. coli, mammalian cell-lines and transgenic mice  Generating DNA constructs for conditional gene-expression in B. subtilis, E. coli, mammalian cell-lines and transgenic mice  RNA extraction from B. subtilis, E. coli and mammalian cells  Generation of cDNA from mammalian RNA  Northern blot  Real time PCR analysis of mammalian gene expression using Taqman and SYBR green chemistry Protein techniques  T7 protein over-production systems  Disruption of cells using a sonicator  Disruption of cells using a French press  Membrane and cytoplasmic fraction preparations  Phase fractionation of membrane proteins using Triton X114  Purification of histidine-tagged proteins using Ni-agarose columns  Purification of GST-tagged proteins using glutathione-sepharose columns  Purification of proteins using ion exchange, gel filtration and ADP- sepharose columns  SDS-PAGE  Western blot  Determination of protein concentrations using various methods
  • 9. CURRICULUM VITAE Mattias Gustafsson Page 9 of 11  Determination of cytochrome P450 concentration by spectrophotometry  Immunocytochemistry  Determination of Kd of Cytochrome P450s for ligands by spectrophotometry  Determination Ki of the transcriptional repressor FatR for various ligands by EMSA  Determination of Kd for FatR using a direct binding assay with a fluorescent fatty acid  Investigating enzyme reactions using spectrophotometric and spectrofluorometric methods  ELISA  Solid phase RIA  Bacterial ligand and antibody binding assays Functional screening assays  Bacterial two-hybrid assays  Investigating PPAR agonist induced transcriptional transactivation using mammalian one-hybrid systems  Using mammalian two-hybrid systems to investigate PPAR/RXR heterodimerisation and transactivation  Investigating PPAR repression and agonist induced activation Bioinformatics  Using the GCG and Staden packages for handling smaller sequencing projects  Using the GCG package for predicting open reading frames  Experience with the GCG, MacVector, Vector NTI and Emboss packages  Using BLAST for finding proteins similar to unknown open reading frames  Using the GCG package for predicting transcriptional terminator structures  In silico design of plasmids  Generating phylogenetic trees using the GCG program package  Predicting transmembrane helices using the GCG package  Using RasMol for looking at 3D structures of proteins Other techniques in microbiology  Gram staining  Typing of bacteria using biochemical and morphological criteria  Determining generation times of bacteria  One step growth curves of phage T4 of E. coli  Titration of bacteriophages  Investigating antibiotic resistance of bacteria Microscopy  General light microscopy  Fluorescence microscopy  Phase contrast microscopy TEACHING  Taught in the laboratory practicals of the following courses given in the biology program at Lund University (three times for each course): five and ten credits Microbiology and ten credits procaryotic molecular genetics.  Organized a week of the Molecular Medicine course of the Biomedicine program at Lund University for two years.  Tutored and wrote problem based learning cases for one-two weeks on the Molecular Medicine course of the Biomedicine program at Lund University (four times).  Tutored for four weeks on the General Pathology course of the Medicine program, Lund University)  Tutored for a half semester on the Homeostasis course of the Medicine program, Lund University.  Tutored for two weeks on the Pathobiology and Pharmacology course on the Biomedicine program, Lund University.
  • 10. CURRICULUM VITAE Mattias Gustafsson Page 10 of 11 MANAGEMENT/SUPERVISION  As a Ph. D. student I supervised a project student.  As a postdoc in Dundee I led and planned the work of a research assistant.  One of my main tasks in Professor Svanborg´s laboratory was to supervise members of the laboratory in molecular biology techniques.  During my appointment as postdoc with Professor Svanborg’s group I did to some extent supervise a Ph.D. student (I was not a formal supervisor). I also supervised two half semester project students (not a formal supervisor).  In my current appointment I have to a large extent supervised a Ph. D. student at Copenhagen University with Professor Lindahl and Prof. John Olsen as the formal supervisors. IT SYSTEMS  Responsible for installing computers and programs in our research group.  Been working with Microsoft Windows (XP, Vista and 7), Mac OS X Mountain Lion.  Microsoft Office (Word, Excel, Power Point).  Experience with the GCG and Emboss packages.  Experience with the Staden package.  In silico cloning with MacVector and VectorNTI SCIENTIFIC PUBLICATIONS Articles 1. Lannergård, J., Kristenssen, B., Gustafsson, M., Persson, J., Norrby-Teglund, A., Stålhammar- Carlemalm, M. and Lindahl, G. (2015) Sequence variability is correlated to weak immunogenicity in Streptococcus pyogenes M protein, MicrobiologyOpen Epub ahead of print 2. Nilsson, O. R., Lannergård, J., Morgan, B. P., Lindahl, G. and Gustafsson, M. C. (2013) Affinity purification of human factor H on polypeptides derived from streptococcal M protein: enrichment of the Y402 variant, PLoS One 8, e81303. 3. Gustafsson, M. C., Lannergård, J., Nilsson, O. R., Kristensen, B. M., Olsen, J. E., Harris, C. L., Ufret-Vincenty, R. L., Stålhammar-Carlemalm, M., and Lindahl, G. (2013) Factor H binds to the hypervariable region of many Streptococcus pyogenes M proteins but does not promote phagocytosis resistance or acute virulence, PLoS Pathogens 9, e1003323. 4. Higgins, L. G., Garbacz, W. G., Gustafsson, M. C., Nainamalai, S., Ashby, P. R., Wolf, C. R., and Palmer, C. N. (2012) Conditional expression of human PPARδ and a dominant negative variant of hPPARδ in vivo, PPAR Research 2012, 216817. 5. Lannergård, J., *Gustafsson, M. C., Waldemarsson, J., Norrby-Teglund, A., Stålhammar- Carlemalm, M., and Lindahl, G. (2011) The hypervariable region of Streptococcus pyogenes M protein escapes antibody attack by antigenic variation and weak immunogenicity, Cell Host & Microbe 10, 147-157. * Please note that I´m shared first author this article. 6. Ragnarsdóttir, B., Jönsson, K., Urbano, A., Grönberg-Hernandez, J., Lutay, N., Tammi, M., Gustafsson, M., Lundstedt, A. C., Leijonhufvud, I., Karpman, D., Wullt, B., Truedsson, L., Jodal, U., Andersson, B., and Svanborg, C. (2010) Toll-like receptor 4 promoter polymorphisms: common TLR4 variants may protect against severe urinary tract infection, PLoS One 5, e10734. 7. Romanowska, M., Reilly, L., Palmer, C. N., Gustafsson, M. C., and Foerster, J. (2010) Activation of PPARβ/δ causes a psoriasis-like skin disease in vivo, PLoS One 5, e9701. 8. Gustafsson, M. C., Knight, D., and Palmer, C. N. (2009) Ligand modulated antagonism of PPARγ by genomic and non-genomic actions of PPARδ, PLoS One 4, e7046. 9. Aits, S., Gustafsson, L., Hallgren, O., Brest, P., Gustafsson, M., Trulsson, M., Mossberg, A. K., Simon, H. U., Mograbi, B., and Svanborg, C. (2009) HAMLET (human alpha-lactalbumin made lethal to tumor cells) triggers autophagic tumor cell death, International Journal of Cancer 124, 1008-1019.
  • 11. CURRICULUM VITAE Mattias Gustafsson Page 11 of 11 10. Brest, P., Gustafsson, M., Mossberg, A. K., Gustafsson, L., Düringer, C., Hamiche, A., and Svanborg, C. (2007) Histone deacetylase inhibitors promote the tumoricidal effect of HAMLET, Cancer Research 67, 11327-11334. 11. Lundstedt, A. C., McCarthy, S., Gustafsson, M. C., Godaly, G., Jodal, U., Karpman, D., Leijonhufvud, I., Lindén, C., Martinell, J., Ragnarsdóttir, B., Samuelsson, M., Truedsson, L., Andersson, B., and Svanborg, C. (2007) A genetic basis of susceptibility to acute pyelonephritis, PLoS One 2, e825. 12. Ragnarsdóttir, B., Samuelsson, M., Gustafsson, M. C., Leijonhufvud, I., Karpman, D., and Svanborg, C. (2007) Reduced toll-like receptor 4 expression in children with asymptomatic bacteriuria, Journal of Infectious Diseases 196, 475-484. 13. Fischer, H., Ellström, P., Ekström, K., Gustafsson, L., Gustafsson, M., and Svanborg, C. (2007) Ceramide as a TLR4 agonist; a putative signalling intermediate between sphingolipid receptors for microbial ligands and TLR4, Cellular Microbiology 9, 1239-1251. 14. Targett-Adams, P., McElwee, M. J., Ehrenborg, E., Gustafsson, M. C., Palmer, C. N., and McLauchlan, J. (2005) A PPAR response element regulates transcription of the gene for human adipose differentiation-related protein, Biochimica et Biophysica Acta-Gene Structure and Expression 1728, 95-104. 15. Stephen, R. L., Gustafsson, M. C., Jarvis, M., Tatoud, R., Marshall, B. R., Knight, D., Ehrenborg, E., Harris, A. L., Wolf, C. R., and Palmer, C. N. (2004) Activation of peroxisome proliferator-activated receptor δ stimulates the proliferation of human breast and prostate cancer cell lines, Cancer Research 64, 3162-3170. 16. Gustafsson, M. C., Roitel, O., Marshall, K. R., Noble, M. A., Chapman, S. K., Pessegueiro, A., Fulco, A. J., Cheesman, M. R., von Wachenfeldt, C., and Munro, A. W. (2004) Expression, purification, and characterization of Bacillus subtilis cytochromes P450 CYP102A2 and CYP102A3: flavocytochrome homologues of P450 BM3 from Bacillus megaterium, Biochemistry 43, 5474-5487. 17. Gustafsson, M. C., and von Wachenfeldt, C. (2001) A novel diffusible substance can overcome the apparent AbrB repression of the Bacillus subtilis fatR promoter, FEMS Microbiology Letters 199, 197-202. 18. Gustafsson, M. C., Palmer, C. N., Wolf, C. R., and von Wachenfeldt, C. (2001) Fatty-acid- displaced transcriptional repressor, a conserved regulator of cytochrome P450 102 transcription in Bacillus species, Archives of Microbiology 176, 459-464. 19. Belitsky, B. R., Gustafsson, M. C., Sonenshein, A. L., and von Wachenfeldt, C. (1997) An lrp- like gene of Bacillus subtilis involved in branched-chain amino acid transport, Journal of Bacteriology 179, 5448-5457. 20. Hansson, M., Gustafsson, M. C., Kannangara, C. G., and Hederstedt, L. (1997) Isolated Bacillus subtilis HemY has coproporphyrinogen III to coproporphyrin III oxidase activity, Biochimica et Biophysica Acta-Protein Structure and Molecular Enzymology 1340, 97-104. Reviews 1. Ragnarsdóttir, B., Fischer, H., Godaly, G., Grönberg-Hernandez, J., Gustafsson, M., Karpman, D., Lundstedt, A. C., Lutay, N., Rämisch, S., Svensson, M. L., Wullt, B., Yadav, M., and Svanborg, C. (2008) TLR- and CXCR1-dependent innate immunity: insights into the genetics of urinary tract infections, European Journal of Clinical Investigation 38 Suppl 2, 12-20. 2. Svanborg, C., Bergsten, G., Fischer, H., Godaly, G., Gustafsson, M., Karpman, D., Lundstedt, A. C., Ragnarsdóttir, B., Svensson, M., and Wullt, B. (2006) Uropathogenic Escherichia coli as a model of host-parasite interaction, Current Opinion in Microbiology 9, 33-39. 3. Palmer, C. N., Gustafsson, M. C., Dobson, H., von Wachenfeldt, C., and Wolf, C. R. (1999) Adaptive responses to fatty acids are mediated by the regulated expression of cytochromes P450, Biochemical Society Transactions 27, 374-378. Ph.D. thesis 1. Gustafsson, M. C. U. (2000) Bacterial cytochromes P450: Studies on cytochromes P450 102A2 and P450 102A3 of Bacillus subtilis. Department of Microbiology, Lund University, Lund, Sweden.