Difference between genomic and cdna library https://microbiologynote.com/difference-between-genomic-and-cdna-library/

1. Microbiologynote.com
https://microbiologynote.com/difference-between-genomic-and-cdna-library/
Genomic DNA library cDNA library
Genomic library is a collection of the clones bearing
the total genomic DNA of an organism.
cDNA library is a collection of the clones bearing
the complementary DNA to the mRNA of an
organism
Genomic library consists of the entire genomic DNA
including noncoding (introns and regulatory) DNA.
cDNA library contains only the coding
sequences; it does not contain introns.
Genomic library is large. cDNA library is small.
The starting material is DNA. Starting material is mRNA
Reverse transcription does not happen. Reverse transcription happens in the first cDNA
strand synthesis.
It include all possible fragments of DNA from a given
cell or organism.
cDNA library carries only expressed gene
sequences.
Vector used genomic library include plasmid, cosmid,
lambda phage, BAC and YAC in order to
accommodate large fragments
Vectors used cDNA library include plasmid,
phagemids, lambda phage etc to accommodate
small fragments as cDNA has no introns.
Restriction endonucleases and ligases are important
for its construction.
Reverse transcriptase enzyme plays an important
role in its construction.
They carry introns also. They lack introns.
They may represent the DNA of both eukaryotic and
prokaryotic organisms.
They represent the DNA of only eukaryotic
organisms.
They are not capable of expression in prokaryotes (like
the bacteria) because they carry introns and
prokaryotes do not have machinery to process introns.
They are capable of expression in bacteria
because they lack introns.