PHYTOCHEMISTRY AND PHARMACOGNOSY II, UNIT III, ISOLATION IDENTIFICATION AND ANALYSIS OF RESIN

1. SUBJECT
PHYTOCHEMISTRY AND
PHARMACOGNOSY II
UNIT III
ISOLATION IDENTIFICATION AND
ANALYSIS OF RESIN
PREPARED BY: SNEHAL UTTAM KASHID.
(Asso. professor)
Department of Pharmacognosy,
SPMs college of pharmacy, akluj

2. Podophyllotoxin

3. Podophyllotoxin
• Podophyllotoxin is the Lactone resin
• synonym : Indian Podophyllum , Himalayan May apple.
• Biological source: dried roots and rhizomes of
Podophyllum emodi, Podophyllum hexandrum
• Family : Berberidaceae
• Other C.C.- podophyllic acid and picropodophyllin, α-
peltalin and β-peltalin.

4. Isolation of podophyllotoxin (method 1)
Take the weighed quantity of rhizomes or roots of
Podophyllum emodi with methanol.
Filter and evaporate to semisolid mass.
Dissolve semisolid mass into acidic water.
Precipitate is formed which should be allowed for at least
for 2hrs

5. Isolation of podophyllotoxin (method 1)
Filter and wash filtrate with cold water.
collect the residue, wash with acidified water and dry to
obtain dark brown amorphous powder.
Extract the residue with hot alcohol. Filter and evaporate
to dryness.
Re- crystallise the residue in benzene to yield
podophyllotoxin

6. Isolation of podophyllotoxin(method 2)
Extract 4gm powder Podophyllum emodi root by stirring
and heating with 100ml of ethanol for about 10 minutes.
Cool and filter the mixture
Re-extract the residue with further 100ml of ethanol
Combine the extracts and evaporate to about 20ml

7. Isolation of podophyllotoxin (method 1)
Combine the extract and evaporate to dryness
Isolate the Podophyllotoxin by preparative TLC
Crystallization at 0C for several days

8. Identification And Analysis
1. Chemical test –
Treat podophyllotoxin with 50% Sulphuric acid it
will show violet – blue colour.

9. Identification And Analysis
2.Thin layer chromatography ( TLC )
• Sample preparation – Dissolved about 1mg of
podophyllotoxin in 1ml of methanol
• Standard drug – Podophyllotoxin
• Stationary phase - Silica gel –G
• Mobile phase – Chloroform : Methanol (90:10)
• for about 6 cm (Only glycosides are separated but aglycone
like podophyllotoxin remains in the region of the front.
• 2nd Mobile phase - Chloroform : Acetone (65:35)
• Detecting agent - vanillin-sulphuric acid
• RF value – for 2nd mobile phase Yellow spot – 0.65

10. 2.Thin layer chromatography ( TLC )

11. Uses
• Podophyllotoxin and its derivatives are used as -antiviral
agent
• Antihelminthic
• Antitumor agents.
• Podophyllotoxin derived antitumor agents include
etoposide and teniposide
• Used in testicular, breast, pancreatic, lung, stomach, and
ovarian cancers.

12. Bibliography
• Kokate, C.K., Purohit, A.P. and Gohkale, S.B.
(2019) Pharmacognosy. In Terpenoids, 57th
Edition, Nirali Prakashan, Pune.
• Pharmacognosy And Phytochemistry By Vinod
Rangari. 2009 2nd edition, Career Publications.