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- 1. Research in a nutshell
- 2. Metalloporphyrins and other complexes as ionophores for hydrophilic anions • Synthesis of novel compounds (mainly Zr-and Al-derivatives) • Studies on the selectivity and other paramaters of developed ISEs
- 3. Metalloporphyrins as ionophores R1 Metals Applied our group Mn3+ Mn3+ BF4 - NO2 CN-Sal- R1 R1 N SCN- F-I-Cl- Ace- - I3 - Cu2+ Zn2+ Mn3+ Fe3+ Rh3+ Cr3+ MoO3+ Lu3+ Sn4+ Mn3+ Mn3+ In3+ Tl3+ Co3+ Rh3+ Ga3+ Zr4+ Al3+ Sc3+ Zr4+ N N N R2 R1 R1 R2 R1 R1 R2 R1 R2 Mn+ R1 - R2 - H R1 - H R2 - R1 - H R2 - R1 - H R2 - Cl Cl R1 - H R2 - NH O
- 4. Application of developed ISEs in FIA systems • Determination of acetylcholine • Determination of voltaile fatty acids • Determination of pesticides Sample (Enzyme substrate, inhbitor, activator) Enzymatic flow-through reactor Detector (acetate selective electrode) Gly buffer (pH 3,0) Phosphate buffer (pH 7,4)
- 5. Silicon-based transducers Working electrode 3-electrode transducer •vacuum deposited gold electrodes •smooth electrode surface facilitate DNA monolayer stability I II III I – bare gold electrode II – ssDNA III - dsDNA
- 6. DNA receptor layers • New redox markers for DNA sensors • Silicon-based transducers for DNA sensors • DNA receptor layers for determination of heavy metal cations • Aptasensors
- 7. s s s s Metalacarboranes as DNA redox labels M M = Fe3+, Cr3+, Co3+
- 8. DNA for heavy metals determination eT eT methylene blue – redox marker UO2 2+ - LDL 30 nmol/L Hg2+ - LDL 5 nmol/L Pb2+ - under investigation
- 9. Metalloporphyrins for labeling of proteins • Various transduction techniques -voltammetry -spectrophotometry -spectrofluorimetry Properties of metalloporphyrins Influence of various proteins (antibodies) on the obtained signals
- 10. Quantum Dots as labels • Modification - hydrophilization and functionalization of surface • Stability • Determination (influence of chosen species (proteins) on obtained optical and electrochemical signals, elimination od undesired effects)
- 11. Other interests… Interactions between DNA, aptamers, peptides, proteins and... • SPR • QM Modification of gold surface (chemisorption, covalent bonding, adsorption)