these slides are prepared for biotechnology student and it is more informative for industrial biotechnology student. Hope you people will get huge knowledge from it.

1. Agriculture
Biotechnology
Ammara Masood
Lecturer
FLS, UCP.

2. Biotechnology
• Biotechnology is a broad discipline in
which biological processes, organisms, cells
or cellular components are exploited to
develop new technologies. New tools and
products developed by biotechnologists are
useful in research, agriculture, industry and
the clinic.

3. What is Biotechnology?
• Biotechnology is the manipulation of
living organisms and organic material to
serve human needs.
• Examples:
– Yeast in bread making and alcohol
production
– Use of beneficial bacteria (penicillin) to kill
harmful organisms
– Cloning of plants and animals
– Artificial insemination

4. Biotechnology Industry
• Research is conducted by small
companies, large corporations, and
public universities.

5. Biotechnology Industry
• Funding comes from a variety of
sources:
– Public (government)
– Private (companies and foundations)
• California passed a $300 billion (1billion
=100 crore)referendum for research in
stem cells in 2004.

6. Biotechnology Industry
• Focuses on a variety of research areas
including:
– Health/medicine
– Food science
– Environmental science
– Agriscience

7. Impact of Biotechnology
• Genetically modified organisms (GMOs)
are consumed by millions of people
(especially Americans) EVERY DAY.
– Almost 56% of all soybean plantings
worldwide are genetically engineered

8. Impact of Biotechnology
• Genetically modified crops were
produced on more than 167 million acres
in 18 countries in 2003, a 15% increase
from 2002
– The US was the largest single producer with
more than 60% of the total acreage in
production.

9. Impact of Biotechnology
• The NC Biotechnology Center predicts
that the biotechnology industry in the
state will contribute more than $25 billion
in annual income each year within the
next 25 years.

10. Pioneers in
Biotechnology

11. Antony van Leeuwenhoek
• 1675
• Discovers bacteria using a simple microscope
• Van Leeuwenhoek discovered "protozoa" - the single-celled organisms
and he called them "animalcules". He also improved the microscope and laid
foundation for microbiology. He is often cited as the first microbiologist to study
muscle fibers, bacteria, spermatozoa and blood flow in capillaries.

12. Gregor Mendel
• 1863
• Austrian monk who
conducted the first
genetics experiments
using pea plants in
the mid 1800s.
• Often considered the
founder of genetics.

13. Louis Pasteur
• 1870’s
Louis Pasteur demonstrated that microorganisms
cause disease and discovered how to make
vaccines from weakened, or attenuated,
microbes. He developed the earliest vaccines
against fowl cholera, anthrax, and rabies
describing the role of
bacteria in spoilage and the
scientific basis for
fermentation

14. Robert Hooke
• 1665
• Invented the compound light microscope
• First to observe cells in cork.
• He used it to study all kinds of microscopic creatures. In
1665 he published his book Micrographia which detailed
his observations and included drawings. ... In
summary, Hooke is important to the study of cells
because he greatly improved the microscope, microscopy,
and coined the term "cell" as we use it today.

15. James Watson & Francis
Crick
• 1953
• Englishmen
responsible for the
discovery of the
double helix
structure of DNA
using X-ray
photographs

16. Paul Berg
• 1972
• Stanford University
scientist who first
developed recombinant
DNA technology, a method
for insertion of genetic
material from one
organism into another.

17. Historical
Development
of
Biotechnolgoy

18. 1750 B.C.
• Origins of “biotechnology” emerge in
methods of food production and plant
and animal breeding
– Use of bacteria to produce cheese (food
preservation)
– Use of natural enzymes in yogurt
– Use of yeast to produce bread
– Use of fermentation for producing wine and
beer

19. 1869
• DNA is discovered in trout sperm by German Miescher.
• In 1869, Friedrich Miescher isolated "nuclein," DNA with associated
proteins, from cell nuclei. He was the first to identify DNA as a distinct
molecule.
• Miescher, a Swiss scientist, wanted to study the chemistry of cells. He
chose to study white blood cells, which are abundant in pus, and were
abundantly available to him in bandages from a hospital near his
university

20. 1919
• The word “biotechnology” is first used
by a Hungarian agricultural engineer.
• Károly Ereky (German: Karl Ereky; October
20, 1878 – June 17, 1952) was
a Hungarian agricultural engineer. The
term 'biotechnology' was coined by him in
1919. He is regarded by some as the
"father" of biotechnology.

21. 1940’s-1950’s
• Widespread work is undertaken to
investigate the structure and function of
DNA

22. 1980
• The U.S. Supreme
Court approves
the patenting of
genetically altered
organisms.

23. 1980’s-1990’s
• A variety of GMO’s and biotechnology
techniques are introduced in fields from
agriculture to medicine
– Recombinant DNA technology-extracts DNA
from one organism for use in another,
allowing more rapid and specific
improvements in plants and animals
– Plant Tissue Culture-gains widespread
acceptance as a method to quickly and
cheaply produce genetically identical plants

24. 1990’s
• First transgenic organisms (GMO’s) are
introduced in widespread agricultural
production, particularly in the area of
crops.
– Bt corn and soybeans are introduced
offering “natural” insect resistance by the
introduction of a gene from the bacterium
Baccillus thuringensis

25. 1997
• Dolly is the first animal cloned from
diploid cells is produced in Scotland

26. Late 1990’s-Early 2000’s
• Human cloning
is outlawed in
the U.S. and the
first concerns
over the use of
human stem
cells in research
begin to arise.

27. Biotechnology
and
Agriscience

28. Biotechnology and
Agriscience
• There has been increased activity and
research between different agricultural
areas with common research techniques
and goals
– Plant Science
– Animal Science
– Environmental Science
– Health/Agri-Medicine

29. Plant Science
• Wide scale
production of
transgenic
plants
impacting
horticulture

30. Animal Science
• Increased use of methods of in vitro
fertilization and artificial insemination
improve selected breed programs

31. Environmental Science
• Use of biotechnology techniques in
environmental science for cleaning
contaminants and protecting endangered
species
– Bioremediation-use of natural organisms to
clean contaminants

32. Environmental Science
• Immunoassay tests are used to test for
the presence of contaminants in soil,
water and even blood
• Installation of biological barriers to
prevent the transfer of harmful
microorganisms between production
facilities
– Example: Tire wash channels

33. Health/Agri-medicine
• Pharming-the creation of plants and
animals capable of producing medical
substances
• The use of biological barriers to prevent
the spread of harmful microorganisms
that could contaminate food sources

34. Health/Agri-medicine
• DNA analysis/paternity testing has
emerged as a technique to test the
genetic ancestry of animals

35. Problems with
Biotechnology
in Agriculture

36. Problems with Biotechnology
• Transfer of genes found in transgenic
organisms to natural populations.
– Terminator genes have been used to
minimize this risk

37. • Unexpected impacts of genetically
modified organisms and biotechnology
processes on other organisms and the
environment
Problems with Biotechnology

38. • Expense of the utilization of many
biotechnology techniques
– Cost of producing transgenic animals (There
are transgenic fish, but no livestock yet.)
Problems with Biotechnology

39. • Concerns over the safety
and ethics of
incorporating GMO’s into
food for human
consumption
– Allergens
– Example: The use of
Starlink corn in taco shells
not approved for human
consumption
Problems with Biotechnology

40. • Lack of education
among both
consumers and
producers
concerning
biotechnology
processes and
products
Problems with Biotechnology

41. Biotechnology
Terms

42. Biotechnology Terms
• Use your biotechnology books to define
the following terms:
– Cloning
– Clonal Offspring
– Deoxyribonucleic acid
– Genetics
– Genetic Engineering
– GMO
– Ribonucleic Acid
– Transgenic Organism