2. Introduction
Nanomaterial
◦ 1 to 100 nanometers
Nanoparticles (NPs)
◦ One type
Drug delivery
◦ Small
◦ Easily diffuse through the cell
◦ Antibodies
3. Overview
The NPs have great potential for biomedical
applications
◦ Size
◦ Fluorescent dye
How do we make the particles better?
How do we effectively utilize them?
Worked with Dr. Korampally and Dr. Elsawa
4. NP Background
Clump together over time
◦ Need to optimize stability
Hydrophobic core
◦ Encases dye
Hydrophilic shell
◦ Water soluble
Traceable in Cells
◦ Fluorescent
6. Methods
Create cores using PMSSQ, rhodamine chloride dye, and PPG
Age 25 days
Create shells using ammonium hydroxide
Age 25 days
Add hydrochloric acid to remove charge
Particle vials
before recovery
8. Methods Cont.
Centrifuge
Add ammonium hydroxide to return charge
Grow 24 wells of Panc-1 cancer cells
NPs after
being
centrifuged
during the
recovery
process
31. Engineering Results
o80 mg of dye is effectively encased in the particles
oDifferent concentrations of dye are being tested to find the optimal
amount
oLow concentrations of dye have already proven unsuccessful and
quickly coagulate
oNPs created remain evenly dispersed throughout the solution
32. Biological Results
oConfirmed the hypothesis that increased quantities of NPs increases
the fluorescence
oWork will be done to pinpoint the time necessary for NP absorption
oDecrease the amount of time wasted
oOptimize productivity and increase quantity of experiments
33. Discussion
oCouple NPs with something toxic to pancreatic cancer cells as a
possible cancer treatment
oSee if attaching different compounds to the NPs enhances the delivery
oCompute NP retention in cells
oCalculate number of dyes per particle
oAnalyze lifetime of NPs at Argonne National Laboratory
