N. Ramkumar is a Project Associate at TERI in New Delhi, working on research projects involving biohydrogen production from renewable sources using dark and photo fermentative bacteria. He has over 6 years of experience in microbiology, including research on biohydrogen production from molasses and isolation of hydrogen-producing bacteria. He also has experience in quality control for a brewery and monitoring water quality. Ramkumar has published two papers in international journals and presented posters at national conferences. He is seeking opportunities to further develop his skills in microbiology.

1. N. RAMKUMAR
Environmental an Industrial Biotechnology
Microbial BiotechnologyArea
6th Floor,The Energy and Resources Institute(TERI)
India Habitat Centre,Lodhi Road
New Delhi-110003
Contact: 08800267280 Email: rammicro74992@gmail.com
To work with an organisation which provides me opportunities to apply and further develop my skills in
the field of Microbiology which could be helpful for development of organization and for my professional
career.
SYNOPSIS
 Working as Project Associate in TERI, EIBD division in Research project funded byCentre
for High Technology and Department of Biotechnology research.The main objective is to
study biohydrogen production from renewable sources using dark and photo fermentative
bacteria.Integrating dark fermentation effluent using Photofermentation process
 Experince in microbiological techniques and laboratories
 Experience in Quality control in brewing industry (beer), anaerobic hydrogen production, and
waste water analysis.(Physico chemical and biological).
 Extensive experience in chemical and biochemical analysis/characterization/estimation of
waste water
 Hands on experience in Gas Chromatography (Agilent 7890A), Fermenter(Bioengineering,
New Brunswick) .
 Hands on experience in handling HPLC (Agilent series) for estimation of sugars.
 Quantification of biogas production by using Gas flow meter (Ritter)
 Anaerobic culture of media preparation for growth of bacteria inGas manifold sparging
instrument using Nitrogen /Argon gas to maintain anaerobic.
 Microbiological manitanence,media preparation,Good Microbiology lab practises
 Two publication in international peer reviewed journals.
 Two poster presentation at National conferences,Association of Microbiologist of India
 M.ScMicrobiology,Sourasthra College, affiliated to Madurai Kamraj University
SKILL Set
Involved actively in Biohydrogen experiment design from Lab scale to Fermenter scale

2.  Major role of this includes isolation,selection of the bacterial strains, substrate utilization,
yield efficiency, parameters and process optimization.
 Head space gas analysis produced during fermentation using gas chromatography
 Volatile fatty acids profile using gas chromatography
 Microbiological media preparation by aerobic and anaerobic
 COD estimation of fermentation samples to analyze yield efficiency
 Head space gas analysis produced during fermentation using GC (Agilent 7890A)
 Volatile fatty acids profile analysis by Gas Chromatography(Agilent 7890)
 Sugar analysis by HPLC(Agilent)
 Biochemical method to study the concentration of Carbohydrates
 Waste water analysis ,quality analysis of beer samples by microbiological and chemical
parameters
 Working in bioreactor (Bioengineering) maximum of 30 litre scale.
 Actively involved in up scaling our biohydrogen maximum of 1000litre
 Apart from my project work, carried out some research experiments which helped to
publish in international journal and to attend national conference.
 Good practices followed in microbiological laboratories.
Education/Academia
 SourasthraCollege,MaduraiKamrajUniversity,Madurai ,Tamil Nadu
Masters ofScienceMicrobiology (2008)
Cumulative Grade Point Average: 67.8/100
 As the course requirement of the M.Sc. program, presented a seminar on “ Studies on Indoor
air borne microbial quality at Card and Research Development (CARD) under
MrShanmugasundaram, Microbiologist(Executive) ,Centre and Research
Development(CARD),Neyveli Lignite Corporation ,Neyveli.
 SrimadAndavan Arts and Science College, BharathidasanUNiversity, Trichy,Tamil Nadu
Bachelor ofScience Degree in Microbiology (2006)
Aggregate Percentage:60.5%
CAREER SCAN

3. June 2011 to Till Date TERI, New Delhi as a Researcher
Designation: Project Associate
Role:
 Integrated approach for biohydrogen production through combined dark and Photo fermentative
process funded by Centre for high technology
 Dark fermentative biohydrogen production from renewable source sponsored by Department of
Biotechnology.
Work profile:
 To carry lab scale and proto scale fermenter study for biohydrogen fermentation
(anaerobic) from Molasses and other renewable sources
 Enrichment ,isolation of hydrogen producing microorganism
 Mother culture maintenance, andsub culturing, storage for further studies.
 Head space gas analysis using Gas Chromatography (Agilent 7890) and to study
metabolites profile.
 Media preparation, process condition optimization for anaerobic fermentation
 Up scaling to maximum 1000litre batch fermentation from ascending order of lab
scale,10litre,30 litre,100 litre. The batch fermentation studies were performed, Bioreactor
(Bioengineering ,Switzerland).
 Data compilation from the work done and helping in report preparation.
 Litreature study and help in paper writing of research achievements in peer reviewd
journals
September 2010 to May 2011 Anna University,Centre for Environmental Studies,Chennai as
Researcher
Designation: Junior Research Fellow
Role:
Monitoring and Database Management of the waterways of Chennai City funded by MoEF(Ministry of
Environment and Forest)
Nature of Work
 To collect waste water sample from Chennai city and from Effluent Treatment Plants
 Insitu and lab analysis of samples

4.  Organic and Inorganic analysis by Chemical method as per APHA
 To estimate the Total Coliforms of the sample and basic microbiological techniques
 Compilation of datas and preparing reports.
Novemnber 2008 toOctober2009M/S EmpeeBreweries,Chennai
Designation: Quality Control (Trainee )
Role: Trainee Chemist/Microbiology
Work profile:
 Sample collection and analysis of Beer from various stages of fermentation
 Monitor the microbial quality of the beer from raw materials to finished beer product
 Chemicals parameters like alcohol,colour,bitterness,pH,haze of the beer to be monitored
 Monitoring the working stations like Fermenter room, Cold Room,Pasturizingarea,and raw
materials storing places to ensure the microbial quality by collecting samples by settle plate
methods in bacterial agar plates.
 Maintaing records of the quality parameters done in proper order.Log book maintain of the
instruments used.
 Preparing samples for government approval for bottling of every batch.
PUBLICATIONS
1.Impact of regulated pH on protoscale hydrogen production from xylose by an alkaline tolerant
novel bacterial strain Enterobacter cloacae DT-1
Sanjuktasubudhi*,TanmayNayak,N.Ramkumar,P.Vijayananth,BanwariLal*
International Journal of Hydrogen Energy.(38)(2013)2728-37
2. Photofermentative hydrogen production by a new strain:Rhodobacter sphaeroides CNT2A,isolated
from pond sediment.
Sanjukta subudhi,Sachin K.Mogal,N.Ramkumar,Tanmay Nayak,Banwarilal,Harshad
Velankar,T.A.Rajiv kumar,Peddy V.C. Rao,Nettem V.Choudary,Gitesh Shah,Sriganesh
International Journal of Hydrogen Energy ;41(2016) 13979-13985.
3.Aquatic plant Azolla as the universal feedstock for biofuel production.
Ana F.Miranda ,Bijoy Biswas,Narasimhan Ramkumar,Rawel Singh,Jitendra Kumar,Anton
James,Felicity Roddick,Banwari Lal,Sanjukta Subudhi,Thallada Bhaskar and Aidyn Mouradov.
Biotechnology for fuels : D01 10.1186/s13068-016-0628-5

5. Poster Presentations
1.Dark fermentative hydrogen production by Enterobactercloacae DT-1, an alkaline tolerant
facultative anaerobe isolated from crude oil contaminated soil. 53rd
AMI Conference,India.
SanjuktaSubudhi, TanmayaNayak, N. Ram Kumar, P.Vijayananth&BanwariLal.2012
2.Impact of C/N ratio on improvement of hydrogen production from molasses by Clostridium
butyricum TM9A
Pulikkal D Anupama1
, Ram N Kumar1
, Sanjukta Subudhi1
, Madan Junghare1
, TanmayaNayak1
and
BanwariLal*
3.Dark fermentative hydrogen production by Clostridium beijerinkii, isolated from Chilika lake
BRSI Conference,ICETB 2014,India
N.Ramkumar,Sachin Mogal,Debojyoti Mazumdar ,Tanmaya Nayak,Sanjukta subudhi and Banwari
lal
Gene AccessionNumber
Accession number at Genebank NCBI (JX885522.1)
Accession number at Genebank NCBI (KF892545)
PERSONAL DOSSIER
Date of Birth 20-12-1985
Nationality Indian
Languages Proficient English, Tamil, Hindi
Address No 249A, Room No: 19, Munirka, New Delhi-110067
Permanent Address D24, Palar Road,Block 17,Neyveli Township.Tamil Nadu,India
Relocate: Yes.
Date 22/11/2016
Place New Delhi
N.RAMKUMAR