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Culturing and inculation techniques used in Bacteriology.pptm.pptx
- 1. Culturing and inculation techniques used in Bacteriology
- 8. pour plate method The pour plate method is a plating technique that is commonly used for obligate and anaerobic bacteria. This technique is used to isolate microbial colonies by serial dilution and then counting the colony forming units (CFUs). In this method, the liquid sample is poured into the petri dish before the solidification of the agar medium. After solidification, colonies grow both inside and on the surface of the medium. However, the colonies growing inside the medium are confluent; those on the surface are used for viable counting
- 11. Types of Streak Plate Method • Quadrant Streaking. It is the most commonly used and the most preferred method where four equal-sized sections of the agar plate are streaked. ... • T-Streaking. ... • Continuous Streaking. ... • Radiant Streaking. ... • Semi-quantitative Streaking. ... • Zigzag Streaking.
- 14. Streak plate method • Streak culture is the most useful method for obtaining discrete colonies of the bacteria. • It is carried out by streaking on the surface of a solid media plate using a platinum or nichrome loop of 2–4 mm diameter. • In this method, a loopful of the inoculum is placed near the peripheral area of the plate. • The inoculum is then spread with the loop to about one-fourth of the plate with close parallel strokes.
- 15. • From the primary inoculum, it is spread thinly over the plate by streaking with the loop in parallel lines. • The loop is flamed and cooled in between the streaks to obtain isolated colonies. • The inoculated culture plate is incubated at 37°C overnight for demonstration of colonies. • Confluent growth occurs at the primary inoculum, but becomes progressively thinner, and well-separated colonies are demonstrated on the final streaks of the inoculum. • Single isolated colonies obtained by this method are very useful to study various properties of bacteria. • Streak culture is the most useful method for obtaining discrete colonies of the bacteria.
- 20. Stab culture • Prepared by puncturing a suitable medium – gelatin or glucose agar with a long, straight, charged wire. • Uses • Demonstration of gelatin liquefaction. • Oxygen requirements of the bacterium under study. • Maintenance of stoke cultures
- 21. Liquid cultures ▪ Liquid cultures are inoculated by touching with a charged loop or by adding the inoculum with pipettes or syringes. ▪ Uses – Blood culture – Sterility tests – Continuous culture methods ▪ Disadvantage – It does not provide a pure culture from mixed inocula.