The document analyzes the structure of the RZZ complex and its role in kinetochore assembly and chromosome segregation during cell division. The RZZ complex is made up of 3 subunits (ROD, Zwilch, ZW10) and functions as a building block of the kinetochore corona. It allows chromosome migration to the poles during cell division through its interaction with kinetochores. The study used techniques like immunofluorescence, SDS-PAGE, transfection, and electroporation to examine levels of Zwilch and the role of the Spindly protein in kinetochore localization and corona expansion. The discussion analyzes previous studies on the interaction between the RZZ complex and other proteins like MAD1-MAD

1. María Paulina Restrepo Urrego
Tercer semestre de medina
Universidad Pontificia Bolivariana
2022-2

2. Kinetochores
ü ¿? They are multilayered structures built
on specialized chro- matin loci named
centromeres.
ü Funtion: are macromolecular that
promote the bi-orientation and
segregation of chromosomes during cell
division
ü Mechanis: The red layer binds to the
chromatin punctually to the complex
Knl1, Mis12 and Ndc80 generating an
interface and union of microtubules.

3. RZZ Complex
ü The RZZ complex is considered the
corona’s building block.
ü It has 3-subunit: ROD–Zwilch–ZW10
ü This complex is the motor that allow
the migration of the chromosomes to
the polos of the cell and together with
the kinetochores allows cell division

4. OBJECTIVE
Analyze the structure of the RZZ
complex and molecular basis of
Spindly-driven corona assembly at
human kinetochores

5. Métodos
Es una técnica que utiliza sustancias
fluorescentes que permiten detectar la
presencia de antígenos o anticuerpos y
en el estudio se utilizó para mirar lo
niveles de Zwilch en cinetocoros de
células HeLa.
Inmunofluorescencia
SDS-PAGE
Es una técnica que se utiliza para
desnaturalizar las proteínas y
mantenerlas desplegadas. En el estudio
se utilizo para incubar la Spindly.

6. Métodos
Es un proceso por el cual se
introducen ácidos nucleicos en
células de mamíferos.
Transfección
Electroporación
Es una técnica que se utiliza para
aumentar la permeabilidad de la
membrana celular. En este caso se utilizo
para facilitar la penetración de los iones
al interior de la célula.

7. Resultados

8. Resultados

9. Discussion
Author The author said Agree or Disagree
Rodriguez-Rodriguez et al.
The MAD1-MAD2 complex, for instance,
requires the RZZ complex for kinetochore
localization, and will localize to the kinetochore
even if the corona cannot expand due to Spindly
depletion.
ü.
Kuijt et al
As kinetochores enrich MPS1 during mitosis,
and MPS1 activity is highest at these structures,
albeit not limited to them polymerization may
become naturally spatially limited to
kinetochores.
ü.
d’Amico et al
The release of ROD from an auto- inhibited
state upon MPS1 phosphorylation, postulated
here, should be considered alongside the
proposed auto-inhibition of Spindly, shown to
involve an intramolecular interaction of its
coiled coils
ü.

10. Conclusion
1. 2.
In conclusion, molecular
biology allows the analysis
and understanding of
research areas, and in this
way discover new worlds
that go beyond human
beings.
Finally, medicine direct by
molecular biology allows us
to understand from
genetics, normal and
pathological biological
processes, and this way
arrive at a future
development of therapies.