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MICROBIOLOGIA.
VESSANT AMBIENTAL
Luis Casado
Núria Gumà
Estades Argó 2014-2015
Departament de Genètica i Microbiologia
1a Setmana
Banc de dilucions
● Consisteix en diluir una mostra
de microorganismes tans cops
com ens interesi.
● Al dia següent, vam contar les
colònies que havien crescut del
banc de dilucions.
SEMBRA DE VIABLES
API 10S
● Serveix per identificar Enterobacteriaceae,
inoculant una suspensió bacteriana
d’aquestes.
● Hi ha 2 mètodes:
○ Ziga-zaga
○ En escocès
SEMBRA PER
ESGOTAMENT
TINCIONS
SIMPLE GRAM
● Serveix per veure la forma, mida i
agrupació dels bacteris.
● Es fa amb blau de metilè.
● Depèn de la composició i estructura de la
paret del bacteri.
● Es fa amb cristall violeta, lugol i safranina.
2a Setmana
● SEDIMENTACIÓ
● IMPACTE (10 o 100 Litres d’aire)
MAS-100 Eco
Sembra de plaques
Resultats
● Observació de bacteris
● Observació de fongs
CONCLUSIONS
● Estem rodejats de microorganismes.
● Alguns són patògens i altres fan funcions
que ens aporten beneficis.
● Els fongs, tenen una mida més gran que els
bacteris.
● Mirant pel microscopi, podem arribar a la
familia o genere dels fongs, cosa que pels
bacteris no es possible.

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Microbiologia (Vessant Ambiental) Estades Argó 2014-2015 a la UAB, fet per: Núria Gumà i Luis Casado

  • 1. MICROBIOLOGIA. VESSANT AMBIENTAL Luis Casado Núria Gumà Estades Argó 2014-2015 Departament de Genètica i Microbiologia
  • 3. Banc de dilucions ● Consisteix en diluir una mostra de microorganismes tans cops com ens interesi. ● Al dia següent, vam contar les colònies que havien crescut del banc de dilucions. SEMBRA DE VIABLES
  • 4. API 10S ● Serveix per identificar Enterobacteriaceae, inoculant una suspensió bacteriana d’aquestes. ● Hi ha 2 mètodes: ○ Ziga-zaga ○ En escocès SEMBRA PER ESGOTAMENT
  • 5. TINCIONS SIMPLE GRAM ● Serveix per veure la forma, mida i agrupació dels bacteris. ● Es fa amb blau de metilè. ● Depèn de la composició i estructura de la paret del bacteri. ● Es fa amb cristall violeta, lugol i safranina.
  • 7. ● SEDIMENTACIÓ ● IMPACTE (10 o 100 Litres d’aire) MAS-100 Eco Sembra de plaques
  • 8.
  • 9. Resultats ● Observació de bacteris ● Observació de fongs
  • 10. CONCLUSIONS ● Estem rodejats de microorganismes. ● Alguns són patògens i altres fan funcions que ens aporten beneficis. ● Els fongs, tenen una mida més gran que els bacteris. ● Mirant pel microscopi, podem arribar a la familia o genere dels fongs, cosa que pels bacteris no es possible.