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PHARMACY
PO
LIC
E
SC
HOOL
PA
RK
Science for a safer world
We support research and
development of pharmaceuticals,
ensuring the safest and most
effective drug development.
We provide referencestandards for other companies
to check for impurities in theirpharma products.
We contribute to diabetestreatment research; ourapproach enables rapidassessment of the qualityof pancreatic cells fortransplantation.
We provideoccupational drugtesting services forcompanies.
We offer testing fora wide range of foodallergens, includingmethods for testing forthe major allergenicproteins.
We test sports supplements for
illegal additives, so that you know the
supplements you take are safe.
We provideenvironmental testing tocheck pollution levels.
We supply Florisilwhich is used forthe production ofLCD screens.
We test
for toxic
chemicals thatcan be foundin cosmetics.
Our proficiency testingscheme supports toymanufacturers aroundthe world for the safeproduction of toys.
Our medication and doping control
testing safeguards the integrity of
competitive animal sports, defends
the breeding line and protects the
welfare of animals and riders.
Our forensics experts are
involved in solving high profile
‘cold’ cases, enabling successful
prosecution of complex cases.
PHARMACY
POLICE
SCHO
OL
PAR
K
Science for a safer world
We support research
and development
of pharmaceuticals,
ensuring the safest
and most effective drug
development.
We produce certified reference
materials to improve clinical treatment
of organ transplant patients.
We develop more
accurate measurement
methods for detecting
infectious diseases and
diseases such as cancer.
We facilitate research
into the genomics
of many areas of
human disease and
development.
We test medicinal and healthcare
samples on behalf of government
to ensure their quality, safety and
regulatory compliance.
We design and manage
funding schemes in health
and medtech R&D for UK
Government to deliver patient
benefit and improve the health
and wealth of the nation.
Our Oil PT scheme
ensures that the fuel and
lubricants in your car are
fit for purpose.
We screen animal
samples for the presence
e to improve
We provide grant management
services for public sector funders to
improve patient health.
We provide environmental
testing, including our Air
proficiency testing scheme to
monitor stack emissions and
levels of pollution.
acheck water
We work with leading
universities to better
understand how diet
relates to complex
disease states such
as cancer and heart
disease.
We provide universities with
cell lines for cancer research
and work together to better
understand how diet relates
to complex disease states
such as heart disease.
Our medication and doping control
testing safeguards the integrity of
competitive animal sports, defends
the breeding line and protects the
welfare of animals and riders.
Our forensics experts
support the Criminal
Justice System,
helping find key
evidence in a wide
range of cases.
Click above to
activate the
interactive
illustration
More work can be found at
www.tamagodesign.co.uk
The day in a world/life project was
meant to familiarise the audience
with the breadth of services and
products that are available from LGC.
The brief was an open brief, where
two excel spreadsheets were
provided with the information the
client wanted to include. I was asked
to do ‘something’ with it, so I chose
to create an animated interactive
illustration. The illustration style, and
the interactive format, were both
new to the company, but proved very
popular.
Print posters were also created in the
same style.
 lucy@tamagodesign.co.uk
More work can be found at
www.tamagodesign.co.uk
I have extensive experience of
different forms of print layout,
includiing everything from flyers,
brochures, posters, small and large
catalogues, banners and other events
signage, as well as non-standard print
formats (for example promotional
materials).
I can take items from concept to
completion, including preflight
checks prior to print. Items are
created adhering to brand guidelines.
My experience also includes
artworking a number of different
languages- from German and
Spanish, to Chinese and Arabic,
among others.
 lucy@tamagodesign.co.uk
Food safety
Allergens
Detecting and identifying allergens effectively is
an important part of surveillance, regulatory and
commercial control of cross contamination, and
food labelling. Quantification of allergens can be
challenging as inconsistent results are sometimes
observed when using different biochemical test
kits from varying sources. Current methods
detect either the allergenic protein (ELISA, mass
spectroscopy) or allergenic food type DNA (PCR).
To meet the demand for a reliable source of
reference for a peanut allergen, LGC have
developed and manufactured a quality control
material kit for the detection of peanut allergen in
food, with a blank material and a matrix material
with peanut protein added at 10 ppm (mg kg-1
).
Organic Contaminants
Contamination in food and food products can
originate from diverse sources.
The increasing adoption of LC-MSMS has
highlighted that ‘matrix effects’ can have a
detrimental impact on measurement. The use
of isotopically labelled compounds as internal
standards can negate these effects.
LGC offers an extensive range of in-house
manufactured organic contaminants standards.
The Dr. Ehrenstorfer range includes approximately
8,000 different products, available as individual
components (neat and in solution) as well as
mixtures.
We also offer a range of organic contaminant
standards from other manufacturers enabling us to
provide a complete range of reference standards
to meet your requirements.
Pesticides
• Organochlorines
• Organophosphates
• Carbamates
• Pyrethroids
• Neonicotinoids
• Quaternary ammonium pesticides
• Sulfonylurea pesticides
Food quality
LGC produces world leading ISO Guide 34
accredited certified reference materials (CRMs),
matrix reference materials and quality control
materials (QCs). Together with our network of
leading international partners, we supply reference
materials for the analysis of proximates, nutritional
elements and vitamins in matrices including:
• meat, fish and vegetable matter
• cereals and grains
• milk and dairy products
• food and drink products
Food nutritional and compositional testing can
be undertaken to demonstrate compliance with
food labelling requirements and to satisfy food
chain requirements such as retailer specifications
and as a part of due diligence and surveillance
programs.
LGC offers an extensive range of reference
standards including:
• food constituents and natural products
- sugars, carotenoids, amino acids
and vitamins
• food additives
- preservatives, antioxidants, sweeteners
and colourings.
Speciation, authenticity
and GMO
Meat speciation
In 2013, the horsemeat scandal exposed
the presence of various species in both raw
and processed meats sold across Europe.
Consequently, meat speciation analysis has
become increasingly important in tackling
food fraud.
Food authenticity
Isotopic methods are now in routine use for food
authenticity testing, based on the measurement
of the stable isotope content (deuterium, 13
C,
18
O, etc.) of food or ingredients. These methods
can provide information on the botanical and
geographical origin of the food product.
Genetically modified organisms (GMOs)
The marketing of GMOs and food or feed derived
from GMO is controlled in many geographic areas.
In the EU, there is a stringent approval procedure
used in the authorisation of such products.
LGC offers a range of reference materials for use
in method validation, calibration and quality control
in meat speciation, food authenticity and GMO
testing.
LGC has played a vital role in
assisting authorities in fighting food
crime by developing a range of
materials used for the detection of
adulteration of lamb and beef.
Consumer confidence in food relies on the
effectiveness of checks to ensure the safety,
consistency and authenticity of food products.
The focus on the integrity of food has increased
globally in recent years and as a
consequence, new regulations
and higher standards on
quality control and testing
are either planned or
being implemented.
Global trade in food and food
products also means that
exporters must meet international
requirements and demonstrate
security of the supply chain.
The reliability and accuracy of
analytical results is essential
to ensuring consistent product
quality and safety.
Accurate monitoring of food
is supported by the use of
reference materials. Participation
in suitable proficiency testing
schemes provides independent
assurance of the competency of
staff and the suitability of equipment,
methods and procedures.
LGC has over 25 years experience in
manufacturing and supplying reference
materials for the food and beverage
sectors.
Food Integrity
Reference standards for testing
food quality, safety and authenticity
reference standards • matrix materials • certified
referencematerials•proficiency
testing•referencestandards•matrixmaterials
LGC Quality – ISO Guide 34 • GMP/GLP • ISO 9001 • ISO/IEC 17025 • ISO/IEC 17043
Questioned documents:
notes for investigators
cocaine • heroin • amphetamine • ketamine • MDMA
• coc
aine
•heroine•amphetamine
These notes provide a guide for
investigating officers and other
persons submitting documents
to the laboratory for expert
examination. They indicate the
types of examination which can be
undertaken, and provide information
on how the investigator can help the
laboratory to do the best job by the
way he/she prepares and submits
cases. The document examiners
at the laboratory are always
available to answer specific queries
from customers, either in person
when visiting the laboratory or by
telephone.
Questioned documents: notes for investigators 7
The following notes describe ideal procedures. It is recognised that
actual conditions (particularly in respect of the degree of cooperation
by the suspect) may place constraints on the investigator. Ideally,
handwriting comparisons should be made of like with like, i.e. questioned
and reference material should have a similar range and content (e.g.
questioned signatures compared with genuine signatures or specimen
signatures in the same name, cursive writing compared with cursive
writing, block letters and numerals compared with block letters and
numerals, etc.).
(a) Specimen writing on request
The best way to achieve comparability is to take specimen writing on
request (controlled specimens) from possible suspects. Unfortunately,
this often results in unnatural writing as it is necessarily obtained under
“test” conditions. Some preparation is therefore needed on the part of the
investigator if the best specimens are to be obtained.
The following table shows the main points to be noted. LGC ‘Z-cards’ and
flow diagrams also contain useful information for taking specimens.
Take specimens from all possible writers and do not indicate which, if
any, you suspect. Leave the document examiner to make his/her own
selection. This can be an important aspect of his/her evidence in the
witness box.
Provide known writing (specimens) of genuine account holders/losers
even if they are not suspected. The document examiner needs to assess
the likelihood of copying in the questioned writing.
Reference material
Specimens on request
1. Take specimens on a document similar to the questioned
document (e.g. a blank photocopied cheque or other appropriate
blank form) or failing this, on plain paper or lined paper. Provide
suspect with a ball-point pen, preferably a well used one.
2. Specify block lettering, cursive writing or disconnected lower
case writing as appropriate.
3. Dictate what is to be written. Do not show the questioned
document to the suspect - any resemblance to the questioned
writing could later be claimed to be due to copying. If there are
several questioned documents dictate from as many as possible
do not just repeat the text of one of the questioned documents
4. Do not give any help with lay-out or spelling. Resemblances
in these areas can be significant, but only if they occur
unprompted.
5. Take specimens on separate sheets (from 10 to 12 if possible)
removing each sheet from suspect’s sight before starting next -
to avoid copying from sheet to sheet and allowing more natural
variation to emerge.
6. Ensure that specimen signatures are taken in the same name(s)
as on the questioned document(s), not just the suspect’s own
name.
How KASP works
1) Assay components:
In further rounds of PCR, levels of allele- specific tail increase. The
fluor labelled part of the FRET cassette is complementary to new tail
sequences and binds, releasing the fluor from the quencher to generate
a fluorescent signal.
2) Denatured template and annealing components – PCR round 1:
C) DNA template (sample)
5’
3’
3’
5’
G/T
B) KASP Master mix
H Q
F Q
5’ 3’ 5’ 3’
3’ 5’
3’ 5’
4) Signal generation – PCR round 3:
3) Complement of allele-specific tail sequence generated
– PCR round 2:
(Reverse primer binds, elongates
and makes a complementary copy
of the allele-1 tail.)
Fluor for non-incorporatedT allele remains
quenched.
H Q
5’ 3’
3’ 5’
Q
Fluor for incorporatedG allele no longer
quenched.
FAM-labelled oligo binds to new complementary tail
sequence and is no longer quenched.
C
G
F
A) KASP Assay mix
Allele-specific forward primers:
allele-2
allele-1
C
A
Reverse primer:
5’
3’5’
3’
5’
3’
C
G
KASP uses three components:test DNA with the SNP ofinterest; KASP Assay Mixcontaining two different, allele-specific, competing forwardprimers with unique tailsequences and one reverseprimer; the KASP Master mixcontaining FRET cassetteplus Taq polymerase in anoptimised buffer solution.
In the first round of PCR, one of the allele-specific primers matches the target
SNP and, with the common reverse primer, amplifies the target region.
Allele-1 tail FAM-labelledoligo sequence
Allele-2 tail HEX-labelledoligo sequence
Common reverse primer
FAM dye
HEX dye
Target SNP
Quencher
H
Q
F
Legend
(allele-2 primer does not elongate)
A
5’
3’
(reverse primer elongates 5’-3’)
5’
(allele-1 primer binds and elongates)
G
C3’
KASP
genotyping technology
extraction • sequencing • genotyping • extraction
•
sequencing•genotyping•extraction
•
sequencing•genotyping•extraction•sequencing•
genotyping
Understanding the
digital picture
When vital information is buried deep in the
complexity of the digital world, you need an
expert to see the clearer picture and uncover
the evidence.
We specialise in civil litigation, corporate
investigation, criminal prosecution and defence.
•	 Largest UK provider of forensic services
•	 Expert analysis and investigation
•	 Secure, purpose built forensic laboratories
•	 Rapid 24 hour response to emergency incidents from
security cleared staff
•	 Straight forward presentation of complex findings.
LGC	in	forensics
www.lgcgroup.com/digitalforensics
Computer
forensics
Mobile phone
forensics
Cell site
analysis
Audio
visual
Questioned
documents
© LGC Limited, 2014. All rights reserved. 3901/LB/0114
More work can be found at
www.tamagodesign.co.uk
Within my current role I have had
experience creating both videos and
animations. I have experience directing
people talking to camera, and making
them feel at ease, as well as knowledge
of the best setups for lighting.
The short documentary about
identifying the soldiers from WWI
using forensic techniques was popular
amongst the scientific audience. I
directed the scientists during the
interview, as well as filming, and used
other footage shot of a ceremony and
old photos, and edited this all together.
The outcomes video was created in
Flash. The information was provided
in a spreadsheet, and I was asked to
create a video from that.
I also have 3D animation and
modelling, as well as 2D Flash
animation experience.
 lucy@tamagodesign.co.uk
Click below to view the outcomes video, or for the Fromelles short
documentary, please visit https://youtu.be/dxE6d6b-IO4.
More work can be found at
www.tamagodesign.co.uk
I am able to create items suitable for
online use. This includes web banners,
interactive items and interactive PDFs.
I previously worked as the designer
for the international webshop for LGC
Standards. I created the site adhering
to the CMS div setup, but changed the
HTML and CSS styling to promote the
company as more modern.
I have also worked as a web
developer and email coder for another
freelance graphic designer, due to my
knowledge of HTML and CSS, as well
as some Javascript. Examples of this
(coding, not design) can be found here:
http://www.memyselfmore.com/
http://www.dudleysplumbing.co/
I also have knowledge of digital
marketing, and have created items
for social media use (for example
backgrounds and post items).
 lucy@tamagodesign.co.uk
More work can be found at
www.tamagodesign.co.uk
Since I first introduced interactivity
as part of the marketing materials for
the company it has been used in a
number of campaigns. The Valentines’
day hospital waiting room campaign
was particularly popular- with an email
click-through rate of 15% for the US
section of the market.
I have also created an animated disc
interface using Flash (and .bat files), for
the more traditional markets.
 lucy@tamagodesign.co.uk
Hover over the different areas of the image to view some interactivity. These animated areas also work as
buttons, with links to related areas.
Other examples of interactive work can be found at:
www.lgcstandards.com/GB/en/BBQ
www.lgcgroup.com/about-us/science-for-a-safer-world/lgc-in-a-day/#.VWwLrUZGsk8
www.lgcgroup.com/about-us/science-for-a-safer-world/lgc-in-the-world/#.VWwL0EZGsk8
More work can be found at
www.tamagodesign.co.uk
The INSIGHT campaign was an
internal campaign to promote some
new software.
Items designed for this campaign
include icons, computer backgrounds
for the software, an email newsletter,
posters, web banners and an
animated video. This video was
created in Flash, using humour to
promote why you would want to use
the software.
 lucy@tamagodesign.co.uk
Taking DNA forensics to the next level.Sophisticated and revolutionary featuresenabling faster and more efficientprocessing, matching and evaluation.
Next geNeratioN DNa aNalysis techNology
FSS DNA INSIGHT is a registered trademark. No part of this publication may be reproduced or transmitted in any form or by any means, electronic or mechanical,
including photocopying, recording or any retrieval system, without the written permission of the copyright holder. © LGC Limited, 2012. All rights reserved.
3454/LB/1212
UK IT Industry Awards 2010IT Project Team of the Year Medallist
• Fewer process stages
• Enhanced C-LIMS integration• Available across sites- allows easier peer review access
• Creates a presentable court packagewith no manual pagination
• More environmentally friendly- less paper
• Fully UKAS Accredited on Go-Live.
More work can be found at
www.tamagodesign.co.uk
I have worked for a small start-up
company as an illustrator/animator
creating 30o
assets for Facebook games
using Flash and Illustrator. These assets
were bought or won by players to
decorate their ‘garden’ or entertain
their ‘pets’.
The company unfortunately no longer
exists, however it had one of the top
ten games on Facebook at the time.
 lucy@tamagodesign.co.uk
More work can be found at
www.tamagodesign.co.uk
SKILLS
Adobe creative suite software
(including InDesign, Photoshop,
Illustrator, Dreamweaver, Flash)
Video editing and animation:
Adobe Flash, Adobe Premiere,
Autodesk 3D programmes.
Web development:
HTML, CSS and basic Javascript.
Freehand drawing skills.
 lucy@tamagodesign.co.uk
Recent employment history
09/2012- ongoing	 LGC, Queens Road, Teddington, Middlesex TW11 0LY
	 graphic & digital designer (with multimedia)
	 • Digital design: including interactive Flash illustration; HTML emails, interactive PDFs, etc.
	 • Print design: including flyers, brochures, posters, exhibition systems, etc.	
	 • Videography (filming and editing) and animation (diagramatic and character)
04/2004- ongoing	 Freelance Designer, web developer & storyboard artist
	 Design & illustration for external clients
	 PREVIOUS CLIENTS INCLUDE:
	 • Wild Howl design (web development)
	 • Resolution TV (storyboards)	
	 • EFitness (website and branding)
05/2012- 07/2012	 Sharkius games, 78-80 Sophia House, London EC1Y 2BJ
	 2d illustrator/animator
	 • Creating 2d Flash assets for computer games
09/2007- 09/2010	 Market Intelligence Ltd, 1 Nine Elms Lane, London SW8 5NQ
	 Junior creative Artworker
	 DUTIES:
	 • Designing print-ready materials and advertisements, also artworking materials
	 • Laying out pages for magazines and retouching photographs
06/2006- 07/2007 	 Pindar Set, 2nd Floor, Whitefriars, Broadmead, Bristol
	 Graphic Artist
Education
2010- 2011	 Bournemouth university, Bournemouth	 Merit
	 MA 3D Computer Animation		
2002- 2005	 University of the West of England, Bristol		
	 BA (Hons) Graphic Design with Animation	 Level 2.1
2001- 2002	 Wimbledon School of Art, Wimbledon		
	 BTEC Foundation Art		 Merit
1999- 2001	 Richmond Upon Thames College, Twickenham	
	 A Level Graphic Design	 B
	 A Level Maths with Statistics	 B
	 A Level Biology			 C
	 AS Level Geology			 B
1994- 1999	 Grey Court Secondary School, Richmond		
	 11 GCSEs			 A*-B

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LucyBaker_portfolio

  • 1. PHARMACY PO LIC E SC HOOL PA RK Science for a safer world We support research and development of pharmaceuticals, ensuring the safest and most effective drug development. We provide referencestandards for other companies to check for impurities in theirpharma products. We contribute to diabetestreatment research; ourapproach enables rapidassessment of the qualityof pancreatic cells fortransplantation. We provideoccupational drugtesting services forcompanies. We offer testing fora wide range of foodallergens, includingmethods for testing forthe major allergenicproteins. We test sports supplements for illegal additives, so that you know the supplements you take are safe. We provideenvironmental testing tocheck pollution levels. We supply Florisilwhich is used forthe production ofLCD screens. We test for toxic chemicals thatcan be foundin cosmetics. Our proficiency testingscheme supports toymanufacturers aroundthe world for the safeproduction of toys. Our medication and doping control testing safeguards the integrity of competitive animal sports, defends the breeding line and protects the welfare of animals and riders. Our forensics experts are involved in solving high profile ‘cold’ cases, enabling successful prosecution of complex cases. PHARMACY POLICE SCHO OL PAR K Science for a safer world We support research and development of pharmaceuticals, ensuring the safest and most effective drug development. We produce certified reference materials to improve clinical treatment of organ transplant patients. We develop more accurate measurement methods for detecting infectious diseases and diseases such as cancer. We facilitate research into the genomics of many areas of human disease and development. We test medicinal and healthcare samples on behalf of government to ensure their quality, safety and regulatory compliance. We design and manage funding schemes in health and medtech R&D for UK Government to deliver patient benefit and improve the health and wealth of the nation. Our Oil PT scheme ensures that the fuel and lubricants in your car are fit for purpose. We screen animal samples for the presence e to improve We provide grant management services for public sector funders to improve patient health. We provide environmental testing, including our Air proficiency testing scheme to monitor stack emissions and levels of pollution. acheck water We work with leading universities to better understand how diet relates to complex disease states such as cancer and heart disease. We provide universities with cell lines for cancer research and work together to better understand how diet relates to complex disease states such as heart disease. Our medication and doping control testing safeguards the integrity of competitive animal sports, defends the breeding line and protects the welfare of animals and riders. Our forensics experts support the Criminal Justice System, helping find key evidence in a wide range of cases. Click above to activate the interactive illustration More work can be found at www.tamagodesign.co.uk The day in a world/life project was meant to familiarise the audience with the breadth of services and products that are available from LGC. The brief was an open brief, where two excel spreadsheets were provided with the information the client wanted to include. I was asked to do ‘something’ with it, so I chose to create an animated interactive illustration. The illustration style, and the interactive format, were both new to the company, but proved very popular. Print posters were also created in the same style.  lucy@tamagodesign.co.uk
  • 2. More work can be found at www.tamagodesign.co.uk I have extensive experience of different forms of print layout, includiing everything from flyers, brochures, posters, small and large catalogues, banners and other events signage, as well as non-standard print formats (for example promotional materials). I can take items from concept to completion, including preflight checks prior to print. Items are created adhering to brand guidelines. My experience also includes artworking a number of different languages- from German and Spanish, to Chinese and Arabic, among others.  lucy@tamagodesign.co.uk Food safety Allergens Detecting and identifying allergens effectively is an important part of surveillance, regulatory and commercial control of cross contamination, and food labelling. Quantification of allergens can be challenging as inconsistent results are sometimes observed when using different biochemical test kits from varying sources. Current methods detect either the allergenic protein (ELISA, mass spectroscopy) or allergenic food type DNA (PCR). To meet the demand for a reliable source of reference for a peanut allergen, LGC have developed and manufactured a quality control material kit for the detection of peanut allergen in food, with a blank material and a matrix material with peanut protein added at 10 ppm (mg kg-1 ). Organic Contaminants Contamination in food and food products can originate from diverse sources. The increasing adoption of LC-MSMS has highlighted that ‘matrix effects’ can have a detrimental impact on measurement. The use of isotopically labelled compounds as internal standards can negate these effects. LGC offers an extensive range of in-house manufactured organic contaminants standards. The Dr. Ehrenstorfer range includes approximately 8,000 different products, available as individual components (neat and in solution) as well as mixtures. We also offer a range of organic contaminant standards from other manufacturers enabling us to provide a complete range of reference standards to meet your requirements. Pesticides • Organochlorines • Organophosphates • Carbamates • Pyrethroids • Neonicotinoids • Quaternary ammonium pesticides • Sulfonylurea pesticides Food quality LGC produces world leading ISO Guide 34 accredited certified reference materials (CRMs), matrix reference materials and quality control materials (QCs). Together with our network of leading international partners, we supply reference materials for the analysis of proximates, nutritional elements and vitamins in matrices including: • meat, fish and vegetable matter • cereals and grains • milk and dairy products • food and drink products Food nutritional and compositional testing can be undertaken to demonstrate compliance with food labelling requirements and to satisfy food chain requirements such as retailer specifications and as a part of due diligence and surveillance programs. LGC offers an extensive range of reference standards including: • food constituents and natural products - sugars, carotenoids, amino acids and vitamins • food additives - preservatives, antioxidants, sweeteners and colourings. Speciation, authenticity and GMO Meat speciation In 2013, the horsemeat scandal exposed the presence of various species in both raw and processed meats sold across Europe. Consequently, meat speciation analysis has become increasingly important in tackling food fraud. Food authenticity Isotopic methods are now in routine use for food authenticity testing, based on the measurement of the stable isotope content (deuterium, 13 C, 18 O, etc.) of food or ingredients. These methods can provide information on the botanical and geographical origin of the food product. Genetically modified organisms (GMOs) The marketing of GMOs and food or feed derived from GMO is controlled in many geographic areas. In the EU, there is a stringent approval procedure used in the authorisation of such products. LGC offers a range of reference materials for use in method validation, calibration and quality control in meat speciation, food authenticity and GMO testing. LGC has played a vital role in assisting authorities in fighting food crime by developing a range of materials used for the detection of adulteration of lamb and beef. Consumer confidence in food relies on the effectiveness of checks to ensure the safety, consistency and authenticity of food products. The focus on the integrity of food has increased globally in recent years and as a consequence, new regulations and higher standards on quality control and testing are either planned or being implemented. Global trade in food and food products also means that exporters must meet international requirements and demonstrate security of the supply chain. The reliability and accuracy of analytical results is essential to ensuring consistent product quality and safety. Accurate monitoring of food is supported by the use of reference materials. Participation in suitable proficiency testing schemes provides independent assurance of the competency of staff and the suitability of equipment, methods and procedures. LGC has over 25 years experience in manufacturing and supplying reference materials for the food and beverage sectors. Food Integrity Reference standards for testing food quality, safety and authenticity reference standards • matrix materials • certified referencematerials•proficiency testing•referencestandards•matrixmaterials LGC Quality – ISO Guide 34 • GMP/GLP • ISO 9001 • ISO/IEC 17025 • ISO/IEC 17043 Questioned documents: notes for investigators cocaine • heroin • amphetamine • ketamine • MDMA • coc aine •heroine•amphetamine These notes provide a guide for investigating officers and other persons submitting documents to the laboratory for expert examination. They indicate the types of examination which can be undertaken, and provide information on how the investigator can help the laboratory to do the best job by the way he/she prepares and submits cases. The document examiners at the laboratory are always available to answer specific queries from customers, either in person when visiting the laboratory or by telephone. Questioned documents: notes for investigators 7 The following notes describe ideal procedures. It is recognised that actual conditions (particularly in respect of the degree of cooperation by the suspect) may place constraints on the investigator. Ideally, handwriting comparisons should be made of like with like, i.e. questioned and reference material should have a similar range and content (e.g. questioned signatures compared with genuine signatures or specimen signatures in the same name, cursive writing compared with cursive writing, block letters and numerals compared with block letters and numerals, etc.). (a) Specimen writing on request The best way to achieve comparability is to take specimen writing on request (controlled specimens) from possible suspects. Unfortunately, this often results in unnatural writing as it is necessarily obtained under “test” conditions. Some preparation is therefore needed on the part of the investigator if the best specimens are to be obtained. The following table shows the main points to be noted. LGC ‘Z-cards’ and flow diagrams also contain useful information for taking specimens. Take specimens from all possible writers and do not indicate which, if any, you suspect. Leave the document examiner to make his/her own selection. This can be an important aspect of his/her evidence in the witness box. Provide known writing (specimens) of genuine account holders/losers even if they are not suspected. The document examiner needs to assess the likelihood of copying in the questioned writing. Reference material Specimens on request 1. Take specimens on a document similar to the questioned document (e.g. a blank photocopied cheque or other appropriate blank form) or failing this, on plain paper or lined paper. Provide suspect with a ball-point pen, preferably a well used one. 2. Specify block lettering, cursive writing or disconnected lower case writing as appropriate. 3. Dictate what is to be written. Do not show the questioned document to the suspect - any resemblance to the questioned writing could later be claimed to be due to copying. If there are several questioned documents dictate from as many as possible do not just repeat the text of one of the questioned documents 4. Do not give any help with lay-out or spelling. Resemblances in these areas can be significant, but only if they occur unprompted. 5. Take specimens on separate sheets (from 10 to 12 if possible) removing each sheet from suspect’s sight before starting next - to avoid copying from sheet to sheet and allowing more natural variation to emerge. 6. Ensure that specimen signatures are taken in the same name(s) as on the questioned document(s), not just the suspect’s own name. How KASP works 1) Assay components: In further rounds of PCR, levels of allele- specific tail increase. The fluor labelled part of the FRET cassette is complementary to new tail sequences and binds, releasing the fluor from the quencher to generate a fluorescent signal. 2) Denatured template and annealing components – PCR round 1: C) DNA template (sample) 5’ 3’ 3’ 5’ G/T B) KASP Master mix H Q F Q 5’ 3’ 5’ 3’ 3’ 5’ 3’ 5’ 4) Signal generation – PCR round 3: 3) Complement of allele-specific tail sequence generated – PCR round 2: (Reverse primer binds, elongates and makes a complementary copy of the allele-1 tail.) Fluor for non-incorporatedT allele remains quenched. H Q 5’ 3’ 3’ 5’ Q Fluor for incorporatedG allele no longer quenched. FAM-labelled oligo binds to new complementary tail sequence and is no longer quenched. C G F A) KASP Assay mix Allele-specific forward primers: allele-2 allele-1 C A Reverse primer: 5’ 3’5’ 3’ 5’ 3’ C G KASP uses three components:test DNA with the SNP ofinterest; KASP Assay Mixcontaining two different, allele-specific, competing forwardprimers with unique tailsequences and one reverseprimer; the KASP Master mixcontaining FRET cassetteplus Taq polymerase in anoptimised buffer solution. In the first round of PCR, one of the allele-specific primers matches the target SNP and, with the common reverse primer, amplifies the target region. Allele-1 tail FAM-labelledoligo sequence Allele-2 tail HEX-labelledoligo sequence Common reverse primer FAM dye HEX dye Target SNP Quencher H Q F Legend (allele-2 primer does not elongate) A 5’ 3’ (reverse primer elongates 5’-3’) 5’ (allele-1 primer binds and elongates) G C3’ KASP genotyping technology extraction • sequencing • genotyping • extraction • sequencing•genotyping•extraction • sequencing•genotyping•extraction•sequencing• genotyping Understanding the digital picture When vital information is buried deep in the complexity of the digital world, you need an expert to see the clearer picture and uncover the evidence. We specialise in civil litigation, corporate investigation, criminal prosecution and defence. • Largest UK provider of forensic services • Expert analysis and investigation • Secure, purpose built forensic laboratories • Rapid 24 hour response to emergency incidents from security cleared staff • Straight forward presentation of complex findings. LGC in forensics www.lgcgroup.com/digitalforensics Computer forensics Mobile phone forensics Cell site analysis Audio visual Questioned documents © LGC Limited, 2014. All rights reserved. 3901/LB/0114
  • 3. More work can be found at www.tamagodesign.co.uk Within my current role I have had experience creating both videos and animations. I have experience directing people talking to camera, and making them feel at ease, as well as knowledge of the best setups for lighting. The short documentary about identifying the soldiers from WWI using forensic techniques was popular amongst the scientific audience. I directed the scientists during the interview, as well as filming, and used other footage shot of a ceremony and old photos, and edited this all together. The outcomes video was created in Flash. The information was provided in a spreadsheet, and I was asked to create a video from that. I also have 3D animation and modelling, as well as 2D Flash animation experience.  lucy@tamagodesign.co.uk Click below to view the outcomes video, or for the Fromelles short documentary, please visit https://youtu.be/dxE6d6b-IO4.
  • 4. More work can be found at www.tamagodesign.co.uk I am able to create items suitable for online use. This includes web banners, interactive items and interactive PDFs. I previously worked as the designer for the international webshop for LGC Standards. I created the site adhering to the CMS div setup, but changed the HTML and CSS styling to promote the company as more modern. I have also worked as a web developer and email coder for another freelance graphic designer, due to my knowledge of HTML and CSS, as well as some Javascript. Examples of this (coding, not design) can be found here: http://www.memyselfmore.com/ http://www.dudleysplumbing.co/ I also have knowledge of digital marketing, and have created items for social media use (for example backgrounds and post items).  lucy@tamagodesign.co.uk
  • 5. More work can be found at www.tamagodesign.co.uk Since I first introduced interactivity as part of the marketing materials for the company it has been used in a number of campaigns. The Valentines’ day hospital waiting room campaign was particularly popular- with an email click-through rate of 15% for the US section of the market. I have also created an animated disc interface using Flash (and .bat files), for the more traditional markets.  lucy@tamagodesign.co.uk Hover over the different areas of the image to view some interactivity. These animated areas also work as buttons, with links to related areas. Other examples of interactive work can be found at: www.lgcstandards.com/GB/en/BBQ www.lgcgroup.com/about-us/science-for-a-safer-world/lgc-in-a-day/#.VWwLrUZGsk8 www.lgcgroup.com/about-us/science-for-a-safer-world/lgc-in-the-world/#.VWwL0EZGsk8
  • 6. More work can be found at www.tamagodesign.co.uk The INSIGHT campaign was an internal campaign to promote some new software. Items designed for this campaign include icons, computer backgrounds for the software, an email newsletter, posters, web banners and an animated video. This video was created in Flash, using humour to promote why you would want to use the software.  lucy@tamagodesign.co.uk Taking DNA forensics to the next level.Sophisticated and revolutionary featuresenabling faster and more efficientprocessing, matching and evaluation. Next geNeratioN DNa aNalysis techNology FSS DNA INSIGHT is a registered trademark. No part of this publication may be reproduced or transmitted in any form or by any means, electronic or mechanical, including photocopying, recording or any retrieval system, without the written permission of the copyright holder. © LGC Limited, 2012. All rights reserved. 3454/LB/1212 UK IT Industry Awards 2010IT Project Team of the Year Medallist • Fewer process stages • Enhanced C-LIMS integration• Available across sites- allows easier peer review access • Creates a presentable court packagewith no manual pagination • More environmentally friendly- less paper • Fully UKAS Accredited on Go-Live.
  • 7. More work can be found at www.tamagodesign.co.uk I have worked for a small start-up company as an illustrator/animator creating 30o assets for Facebook games using Flash and Illustrator. These assets were bought or won by players to decorate their ‘garden’ or entertain their ‘pets’. The company unfortunately no longer exists, however it had one of the top ten games on Facebook at the time.  lucy@tamagodesign.co.uk
  • 8. More work can be found at www.tamagodesign.co.uk SKILLS Adobe creative suite software (including InDesign, Photoshop, Illustrator, Dreamweaver, Flash) Video editing and animation: Adobe Flash, Adobe Premiere, Autodesk 3D programmes. Web development: HTML, CSS and basic Javascript. Freehand drawing skills.  lucy@tamagodesign.co.uk Recent employment history 09/2012- ongoing LGC, Queens Road, Teddington, Middlesex TW11 0LY graphic & digital designer (with multimedia) • Digital design: including interactive Flash illustration; HTML emails, interactive PDFs, etc. • Print design: including flyers, brochures, posters, exhibition systems, etc. • Videography (filming and editing) and animation (diagramatic and character) 04/2004- ongoing Freelance Designer, web developer & storyboard artist Design & illustration for external clients PREVIOUS CLIENTS INCLUDE: • Wild Howl design (web development) • Resolution TV (storyboards) • EFitness (website and branding) 05/2012- 07/2012 Sharkius games, 78-80 Sophia House, London EC1Y 2BJ 2d illustrator/animator • Creating 2d Flash assets for computer games 09/2007- 09/2010 Market Intelligence Ltd, 1 Nine Elms Lane, London SW8 5NQ Junior creative Artworker DUTIES: • Designing print-ready materials and advertisements, also artworking materials • Laying out pages for magazines and retouching photographs 06/2006- 07/2007 Pindar Set, 2nd Floor, Whitefriars, Broadmead, Bristol Graphic Artist Education 2010- 2011 Bournemouth university, Bournemouth Merit MA 3D Computer Animation 2002- 2005 University of the West of England, Bristol BA (Hons) Graphic Design with Animation Level 2.1 2001- 2002 Wimbledon School of Art, Wimbledon BTEC Foundation Art Merit 1999- 2001 Richmond Upon Thames College, Twickenham A Level Graphic Design B A Level Maths with Statistics B A Level Biology C AS Level Geology B 1994- 1999 Grey Court Secondary School, Richmond 11 GCSEs A*-B