Strategies for Landing an Oracle DBA Job as a Fresher
Tony beristain helsingor bondtalk2
1. Profiling the mouse brain using fluorescent immunolabeling Tony Beristain Ph.D. Tissue Profiling Facility Science for Life Laboratory KISP (Karolinska Institute Science Park)
2.
3. Protein distribution profile in the adult CNS Regions Cell types Subcellular Organelle Lundberg & Uhlén, Proteomics, 2010 Subcellular Protein Atlas Mouse brain
7. Fully automated IHC Capable of processing big volumes Speed Efficiency Reproducible quality Simplicity of operation No daily maintenance Widely used in several hospital around the world
8.
9.
10.
11.
12.
Editor's Notes
25,000 protein coding genes in human: Human genome (2.9 Gbs) only slightly bigger than rat (2.75 Gbs) and mouse (2.6 Gbs) genome Most human genes have homologues in rodent Tissue availability is no issue Rodent brain tissue can be processed under ‘perfect’ conditions Size of the rat/mouse brain allows us to investigate many brain nuclei on a single section (13-10 mm) Relating molecular properties to cellular functions. 13,154 available antibodies Offering are services to analyse protein distribution in animal and human tissue
Cell types: Neural, Non neural Basic cellular distribution such: Nucleus, soma, dendrites, axons, synapses.
Automated image acquisition of full brain sections Automated annotation and quantification of immunoreactivity
The research functionality of the Leica Bond fully automated stain- ing system is an ideal support to the research laboratory, offering consistency and standardisation over conventional manual research techniques and between different researchers working within the same field The number one benefit of any staining system must be the quality; it must produce a very high quality stain. Other factors also include TAT, cost of reagents and programmability with ease-of-use. The system should further standardize your laboratory process, eliminating errors and eliminating manual processes.
With Bond we have double our work flow…generally with a hand made IHC we could process 3 antibodies in one and a half day. With Bond up to 6. On the top of this we can process extra antibodies running a overnight protocol. When it comes to the quality of staining I have to say that is clean and good, although we have encountered a problem which so far we haven’t been able to solve. But I will talk about that later. An extra feature that I do like is that the Covertile technology helps retain tissue on the slide, and is a gentle process compared with the hand made IHC in which between washes some sections are lost - As additional benefits I would mention the cost and time savings. We use 150 μL of each reagent for the entire slide on every slide no matter how much of the slide has tissue.
![Aims and Objectives ,[object Object],[object Object],[object Object],[object Object]](data:image/gif;base64,R0lGODlhAQABAIAAAAAAAP///yH5BAEAAAAALAAAAAABAAEAAAIBRAA7)