1. 1
James Jukosky, Ph.D.
Assistant Professor
Colby-Sawyer College, Natural Sciences Department, 108A Curtis L. Ivey Science Center, 41 Main Street,
New London, NH
Phone: 603.526.3379
E-mail: james.jukosky@colby-sawyer.edu
Education
Dartmouth College, Hanover, NH June 2007
Ph.D. in Molecular, Cellular, and Systems Physiology
Dissertation Title: “The Effects of Persistent and Pseudopersistent Pollutants on Reproduction in Fish and
Ceriodaphnia dubia”
University of New Hampshire, Durham, NH May 2000
B.S. Zoology
Graduated Cum Laude
Awards
NH-INBRE Project July 2013-2015
“Developing novel antimicrobials derived from burying beetles”
Center for Environmental Health Sciences Pilot Grant July 2009-2010
Flight Attendant Medical Research Institute Young Clinical Scientist Award June 2008-2013
“The Influence of Second Hand Cigarette Smoke on the Innate Immune Function of Nasal Epithelial Cells”
Ruth L. Kirschstein Postdoctoral Fellowship in Immunology 2007 to 2008
Best Student Poster Presentation, June 2007
Society of Environmental Toxicology and Chemistry, North Atlantic Chapter Meeting
Albert J. Ryan Fellowship May 2005
Superfund Basic Research Fellowship 2001-2003
NIH Training Fellowship August 2000
Research Experience
Project Leader/ Primary Investigator July 2013-
Colby-Sawyer College, Department of Natural Science, New London, NH
• Led team of undergraduate researchers developing novel antimicrobial peptides
• Performed various antimicrobial and cytotoxicity assays to characterize peptides
Postdoctoral Fellow/ Instructor July 2007 to September 2013
Dartmouth Medical School, Department of Microbiology/ Immunology, Hanover, NH
• Engaged in research studying the effects of cigarette smoke on the innate immune system
• Studied the effects of arsenic on immune function
o Collected blood samples and performed lymphocyte proliferation assays, cytotoxicity
assays, gene expression assays, and immunophenotyping
• Established lab as a DNA construct producing core facility, using recombineering techniques
2. 2
Research Experience Continued
• Designed and built DNA constructs for functional genomics studies
• Lead team of undergraduate students participating in research
• Developed expertise in primary epithelial cell culture
Dissertation Research December 2003-June 2007
Dartmouth College, Department of Physiology, Hanover, NH
• Measured the effects of a variety of pollutants on fish and invertebrate reproduction and
development
• Modeled the effects of contaminant mixtures on fish reproduction
• Characterized molecular biomarkers of contaminant exposure
• Developed and implemented plans for the proper handling of highly hazardous materials
• Gained expertise in standardized toxicity tests and modified these protocols to study subtle
sublethal effects of contaminants
Graduate Student Research Assistant May 2001- 2003
Dartmouth College, Department of Biology, Hanover, NH
• Participated in the investigation of trace metal accumulation in freshwater food webs
• Lead research teams in the lab and in the field
• Completed graduate coursework in ecology, physiology, and statistics
• Acquired expertise in statistical analysis and the use of major statistical software
• Investigated and analyzed data and prepared written summaries for publication
Graduate Student Research Lab Rotations
Dartmouth Medical School, Department of Physiology, Hanover, NH August 2000-May 2001
• Examined the effects of endocrine disruptors on neural development in Xenopus tadpoles
• Performed in vitro fluorescence microscopy on neural crest cell cultures
• Studied molecular mechanisms of thyroid hormone action in the eyes of metamorphic tadpoles
• Performed Yeast 2 hybrid assays
Research Assistant winter-spring 2000
University of New Hampshire, Department of Zoology, Durham, NH
• Collected beetles from the field
• Studied burying beetle behavioral ecology and breeding patterns
• Performed juvenile hormone radiochemical assays
Process Development Intern May 1999-June 2000
Stryker Biotech, Lebanon, NH
• Performed cell culture (CHO cells), purification, and equipment maintenance in the
production of the OP-1 protein
• Helped to set up and run newly built process development laboratory
Teaching Experience
Assistant Professor fall 2010-
Colby-Sawyer College
• Designed and taught the following courses: Bio106: The Chemical and Cellular Basis of Life, Bio205: Human Anatomy and
Physiology I, Bio206: Human Anatomy and Physiology II, Sci107: Science Seminar, Bio333: Immunology, Bio327: Lab
Techniques in Biology, and Bio217: Organismal Physiology
• Fulfilled role as academic advisor
• Mentored undergraduate research
• Attended workshops and training in pedagogical techniques through our Teaching Enrichment Center
• Implemented these techniques in the classroom and lab
• Participated in broader campus-wide initiatives (example: liberal education review)
3. 3
Teaching Experience Continued
Health Studies Coordinator (2011-2012)
• Coordinated the classes that support the Nursing, Exercise Science, Athletic Training, and Health Studies majors
o Scheduled courses in consultation with the department chair.
o Coordinated courses among multiple sections to maintain consistent content.
o Hired, oriented, mentored, and evaluated adjuncts.
o Recommended appropriate changes in the program to the department chair and inform chair of potential impact of
requirements for academic program on Colby-Sawyer (budget, programs, etc.).
o Ordered commonly used lab materials and recommend capital requests needed for these courses
Adjunct Professor fall 2006
Plymouth State University, NH
• Designed syllabus and lectures for general biology course
• Improved curriculum by incorporating specific student interests into lecture and activities
o For example, chapters on biotechnology were supplemented with lessons on
fermentation, DNA fingerprinting, and the human genome project.
o Most lectures began with a recent television, newspaper, or radio clip on a science topic
that related to class.
• Taught a diverse body of students including adult learners and college age students
Graduate Student Teacher Training Workshop April 2006
Dartmouth Center for the Advancement of Learning
Dartmouth College Hanover, NH
• Attended workshop series on active learning techniques
• Designed and implemented college biology lessons based on active learning principles
Women in Science Program (WISP) Assistant Sponsor January-June 2006
• Mentored two WISP students working on research projects in lab
• Presented our research at annual symposium
Guest Lecturer October 2003
Plymouth State, NH
• Presented lecture to 1st
year biology class on wildlife and endocrine disruptors
• Conveyed scientific concepts and contemporary research to introductory level biology students
Teaching Assistant for Animal Behavior March-June 2002
Dartmouth College, Hanover, NH
• Taught laboratory, gave introductory lectures, and led discussions
• Implemented in-class experiments, exercises, worksheets, and exams
• Assisted in independent student research projects
Outreach Educator fall 2002 and 2003
Indian River School, Canaan, NH
• Taught lessons on environmental pollution to 75 5th
graders
• Designed lesson in which students acted out the concept of pollution biomagnification in an
aquatic food web
Volunteer Activites
Board Member
Canaan Conservation Commission, Canaan, NH 2007-2008
Singer and Soloist 2004-2006
Shakers Singers, Enfield, NH
Board Member 1998-present
Brundage Foundation, Newark, NJ
4. 4
Publications
Sturup S., Chen C., Jukosky J., Folt C. (2005). Isotope dilution quantification of Hg-200(2+)
and (CH3Hg+
)-Hg-201 enriched species-
specific tracers in aquatic systems by cold vapor ICPMS and algebraic de-convoluting. International Journal Of Mass Spectrometry
242, 225-231.
Jukosky, J., Watzin, M., Leiter, J.C. (2008) The effects of environmentally relevant mixtures of estrogens on medaka reproduction.
Aquatic Toxicology. 85, 323-331.
Jukosky, J., Watzin, M., Leiter, J.C. (2008). Elevated concentrations of ethinylestradiol, 17 beta-estradiol, and medroxyprogesterone
have little effect on reproduction and survival of Ceriodaphnia dubia. Bulletin of Environmental Contamination and Toxicology. 81,
230-235.
Crane-Godreau M., Maccani, M., Eszterhas, S., Warner, S., Jukosky, J., Fiering, S. (2009) Exposure to Cigarette Smoke Disrupts
CCL20-Mediated Antimicrobial Activity in Respiratory Epithelial Cells, The Open Immunology Journal, 8, 86-93.
Crane-Godreau, M. A., Black, C. C., Giustini, A. J., Dechen, T., Ryu, J., Jukosky, J. A., Lee, H.-K., et al. (2013). Modeling the
influence of vitamin D deficiency on cigarette smoke-induced emphysema. Frontiers in physiology, 4, 132.
doi:10.3389/fphys.2013.00132
Jukosky, J., Fiering, S., Crane-Godreau M., (submitted to Frontiers in Physiology). Cigarette Smoke exposure decreases CCL20 and
beta defensin-2 secretion from primary human nasal epithelial cells.
Presentations
Poster Presentations
NH-INBRE Meeting
“Antimicrobial effects of Burying Beetle Secretions” August 2013
Flight Attendant Medical Research Institute Symposium May 2012
“Cigarette Smoke Exposure Alters Antimicrobial Secretions in Upper Airway Cells”
Flight Attendant Medical Research Institute Symposium May 2009
“The Effect of Cigarette Smoke on Antimicrobial Gene Expression in the Upper Airway”
Flight Attendant Medical Research Institute Symposium May 2008
“Cigarette Smoke Alters Uterine Immune Defense.”
Society of Environmental Toxicology and Chemistry, North Atlantic Chapter Meeting, June 2007
“The Effects of Methylmercury and Aroclor 1242 on Fathead Minnow Reproduction.”
Superfund Basic Research Symposium, Dartmouth College, November 2003
“Metabolic Rate as a Predictor of Mercury Concentration in Freshwater Zooplankton Species.”
Seminar Presentations
New England College, Science Seminar March 2012
“Arsenic Immunotoxicology”
Colby-Sawyer College, Science Seminar February 2011
“Endocrine Disruptors.”
Dartmouth College, Department of Microbiology and Immunology April 2010
5. 5
“The Effects of Arsenic on T-Cell Proliferation.”
Thesis Defense, Dartmouth College, Department of Physiology, May 2007
“The Effects of Persistent and Pseudopersistent Pollutants on Reproduction in Ceriodaphnia dubia and Fish.”
Dartmouth College, Department of Physiology, January 2007
“The reproductive effects of ethinylestradiol, 17-β estradiol, and nonylphenol exposure on pair-breeding Medaka.”
Ryan Foundation Symposium, May 2005
“The Effects of Dietary Methylmercury or PCB Exposure on Reproduction in Fathead Minnows.”
Dartmouth College, Department of Physiology, March 2004
“Mercury: factors affecting biomagnification in aquatic ecosystems and reproductive effects on fish.”
Dartmouth College, Department of Biology, April 2003
“Mercury, Macrozooplankton, and Metabolism”
Dartmouth College, Department of Physiology, September 2002
“Toxic Metal Accumulation in Freshwater Zooplankton”