Bacteriophages Isolated From Turkeys Infecting Diverse Salmonella Serovars Tercer semestre Universidad Pontificia Bolivariana

1. Juliana Rojas Ríos
Tercer semestre de medicina
Universidad Pontificia Bolivariana

2. Some people may develop reactive arthritis
which causes joint pain, eye irritation, and
painful urination.
Salmonellae are anaerobic gram-negative
bacilli, which vary in length.
Salmonella is one of the leading causes
of foodborne illnesses worldwide
The disease caused by Salmonella is
known as salmonellosis, which is a type
of gastroenteritis characterized by
acute onset of fever, abdominal pain,
diarrhea, nausea, and vomiting
INTRODUCTION
SALMONELLA

3. Bacteriophages are viruses that naturally infect
bacteria and behave as obligate intracellular
parasites that multiply by making use of the
bacterial biosynthetic machinery..
Bacteriophages replicate autonomously, carry genetic
information and can confer new properties to bacteria or
develop pathological processes.
Recent studies have shown that
bacteriophages are the most promising
antibacterial agents for biocontrol in foods
because phages specifically kill target
bacteria without affecting other bacteria, do
not alter organoleptic properties or
nutritional quality of foods, and are safe and
environmentally friendly
INTRODUCTION
BACTERIOPHAGE

4. GENERAL OBJECTIVE
In this
work, we isolated and characterized Salmonella phages obtained
from turkeys, and evaluated the potential of the phage with the
broadest and very unusual host range as an effective biocontrol
agent against multiple Salmonella serovars in cucumber juice and beef broth
as model food systems.

5. MÉTODOS
Detección de los fagos
Permite saber si en un determinado
compuesto existe la presencia de cualquier
tipo de sustancia determinada, se hace con
color y en este caso se utilizó para saber si
en las salmonelas ya estaba el fago
Purificación de los fagos
Se hace para separar los ácidos
nucleicos, en este caso fagos,
manteniendo su integridad y evitando
que otros compuestos interfieran en el
resto del proceso

6. MÉTODOS
Extracción
Método por el cual se obtiene el ADN
a partir de material genético o en este
caso de microorganismos con el fin de
poder estudiarlos, manipularlos y
analizarlos
Restricción
Las restricciones se hacen para saber la
variabilidad genética del microorganismo,
entonces, se corta con enzimas que
generan polimorfismos genéticos, en este
caso, para saber la variabilidad de la
salmonela y de los fagos.

7. RESULTADOS

8. RESULTADOS

9. Author The author said Agree o desagree
Chan and Abedon
Phages that produce turbid plaques, therefore, may not be good
candidates for use as biocontrol agents
Kalatzis
The similarity in host ranges may indicate that these phages recognize similar
receptors in the hosts
Sharma, Fong and
Picozzi
To be considered as excellent candidates for use as biocontrol agents in
foods, phages should be strictly lytic and possibly have a broad host range
DISCUSSION

10. CONCLUSIONS
Molecular biology and
research helps to
understand the cellular and
molecular origin of many
pathologies, giving an
enormous contribution to
the development of
medicine.
In the same vein, research
and biology allow us to
find the cure for many
pathologies or the most
appropriate and specific
forms of treatment.
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