It Just Makes Scents…Odor delivery with the 220A Olfactometer

•Download as PPTX, PDF•

0 likes•613 views

During this Tech Cast, produced with Aurora Scientific, Chris Rand discusses the 220A - Olfactometer, a lab tested piece of equipment with proven performance for demanding olfactometry research. He outlines system hardware, configurations and modifications that can be made to the device, and discusses how the olfactometer works. For each section of the tech cast, Chris walks viewers through demonstrations to identify best practices as well as sharing tips and tricks for optimal performance. For more information, visit: https://insidescientific.com/techcasts/it-just-makes-scents-aurora-scientific

Technology

It Just Makes Scents…
Odor delivery with the
Olfactometer
www.aurorascientific.com
Chris Rand MSc
Product Consultant
Aurora Scientific

220A Olfactometer
How does it work?
• Hardware
2| Performance. Precision. Progress.
How do you use it?
• Olfactometer control
How can I replace or add
components?
• Configuration and Modification

• Mass Flow Controllers - how many
(why 3), what do they do, how are
they controlled?
• Connections - what are they, what
do they do, how do they integrate
with external hardware/software?
• LED Indicators – what do they
mean?
• Valve manifold - solenoid valves for
each vial, mixing and final valves
3| Performance. Precision. Progress.
Hardware

• Quick Start Guide - initial setup
• Adding Modules - expanding
from 4 to 8
• Replacing Odorant – 3 methods
• Replacing Tubing
7| Performance. Precision. Progress.
Configuration Changes

• Quick Start Guide - initial setup
• Adding Modules - expanding
from 4 to 8
• Replacing Odorant – 3 methods
• Replacing Tubing
8| Performance. Precision. Progress.
Configuration Changes

• Quick Start Guide - initial setup
• Adding Modules - expanding
from 4 to 8
• Replacing Odorant – 3 methods
• Replacing Tubing
9| Performance. Precision. Progress.
Configuration Changes

• Quick Start Guide - initial setup
• Adding Modules - expanding
from 4 to 8
• Replacing Odorant – 3 methods
• Replacing Tubing
10| Performance. Precision. Progress.
Configuration Changes

11| Performance. Precision. Progress.
1. System Configuration
• Vial Configuration
• Setting Flow Rates
2. Advanced Status and Control
3. Generating Sequences
4. Executing Odor delivery
Olfactometer
Control

Olfactometer Control
Demonstration
| Performance. Precision. Progress.

Summary: an Olfactometer that…
 Delivers precise, repeatable odor profiles
 Is modular that can be easily configured to your needs
 Utilizes three mass flow controllers (MFCs) for repeatable and
reproducible odorant delivery
 Easily integrates into existing behavioral or electrophysiology
experiments
13| Performance. Precision. Progress.

Similar to It Just Makes Scents…Odor delivery with the 220A Olfactometer

MES systems

Barry Curry

Modern business drivers are continually pushing to reduce the time it takes to get a product or service to market, reduce the risk and cost associated with that, and to improve quality In laboratories, delivering an analytical result that’s ‘right first time’ (RFT) is the answer - No reprocessing data or re-running injections - No out of specification (OOS) results or reporting/calculation errors Using chromatography tools for RFT analysis automatically gives: - High quality of results and confidence in results - Lower cost of analysis - Improved lab efficiency - Faster release to market and return on investment (ROI)

Chromatography Data System: Getting It “Right First Time” Seminar Series – P...

Chromatography & Mass Spectrometry Solutions

Instrumentation and control series

Mike Miller

When it comes to user experience a snappy application beat a glamorous one. Nothing frustrates an end user more than a slow application. Did you know that any wait time greater than one second will break a user's concentration and cause them to feel frustration? How can we create applications to meet user expectations? This class will cover all things performance from design to delivery. We will go over application design, user interface guidelines, caching guidelines, code optimizations, and query optimizations.

Optimizing Application Performance - 2022.pptx

JasonTuran2

Migration Concentrate

InnoTech

Wix Dev-Centric Culture And Continuous Delivery

Aviran Mordo

Learning Unit 5_PROCESS CONTROL.pptx

KhothatsoMokoma

Adoption of MIPI standards is accelerating, making design verification and interoperability critically important. In this presentation, Ross Nelson of Protocol Insight discusses effective verification processes to test the physical, link and application layers and the complete system, focusing on debug, stress/corner case testing and conformance/interoperability verification. This presentation also covers effective verification processes and the new MIPI Product Registry model.

MIPI DevCon 2016: Robust Debug and Conformance Verification Ensures Interoper...

MIPI Alliance

Process Control Improvement Primer - Greg McMillan Deminar

Jim Cahill

Functional Design Specification of Hyperglass Cleaning

ShwetonKedia

Epitome Corporate PPT

Epitome Technologies Training

matt heinzelman software quality assurance presentation technical & tool

CuongHoang80

eeMba ii pmom_unit-3.2 modern pm tools a

Rai University

PAC 2019 virtual Joerek Van Gaalen

Neotys

Full report of hyperglass cleaner

ShwetonKedia

Full report of hyperglass cleaner

ShwetonKedia

Oracle Analytics Server Infrastructure Tuning guide v2.pdf

sivakodali7

Modern business drivers are continually pushing to reduce the time it takes to get a product or service to market, reduce the risk and cost associated with that, and to improve quality. In laboratories, delivering an analytical result that’s ‘right first time’ (RFT) is the answer. There is no reprocessing data or re-running injections and no out of specification (OOS) results or reporting/calculation errors. Using chromatography data system tools for RFT analysis automatically gives high quality of results and confidence in results, lower cost of analysis, improved lab efficiency, and faster release to market and return on investment (ROI).

Chromatography Data System: Getting It “Right First Time” Seminar Series – Pa...

Chromatography & Mass Spectrometry Solutions

PID loop optimisation: the benefits of using PROFIBUS PA instrumentation - ...

PROFIBUS and PROFINET InternationaI - PI UK

Providing SharePoint Solutions in an FDA Regulated Environment

Deb Walther

Similar to It Just Makes Scents…Odor delivery with the 220A Olfactometer (20)

MES systems

Chromatography Data System: Getting It “Right First Time” Seminar Series – P...

Instrumentation and control series

Optimizing Application Performance - 2022.pptx

Migration Concentrate

Wix Dev-Centric Culture And Continuous Delivery

Learning Unit 5_PROCESS CONTROL.pptx

MIPI DevCon 2016: Robust Debug and Conformance Verification Ensures Interoper...

Process Control Improvement Primer - Greg McMillan Deminar

Functional Design Specification of Hyperglass Cleaning

Epitome Corporate PPT

matt heinzelman software quality assurance presentation technical & tool

eeMba ii pmom_unit-3.2 modern pm tools a

PAC 2019 virtual Joerek Van Gaalen

Full report of hyperglass cleaner

Oracle Analytics Server Infrastructure Tuning guide v2.pdf

Chromatography Data System: Getting It “Right First Time” Seminar Series – Pa...

PID loop optimisation: the benefits of using PROFIBUS PA instrumentation - ...

Providing SharePoint Solutions in an FDA Regulated Environment

More from InsideScientific

Join Prof. Victoria Hutter and Dr. Louis Scott as they showcase the application of high-content imaging and advanced cell lines for drug safety assessment. Safety concerns play a significant role in the unsuccessful progression of candidate compounds in the later stages of drug development. Establishing the connection between in vitro endpoints and human health outcomes is essential. In this webinar, Prof. Victoria Hutter and Dr. Louis Scott present a novel tool for in vitro safety assessment in drug development. The morph_ONE™ assay provides a human-centric approach to potentially fill specific regulatory gaps concerning safety issues. This tool is capable of profiling both human and rat alveolar macrophages, offering valuable insights for hazard identification and toxicity assessments. By bridging the divide between cellular effects and overall risk, it has the potential to enhance our understanding of safety-related aspects in drug development. Key Topics Include: - Explore distinct in vitro screening techniques for evaluating the safety of emerging inhaled products, facilitating early and informed decisions in compound selection and development. - How high-content image analysis (HCIA) cell painting assays can be used as a forward-looking high-throughput screening tool, distinguishing unique response patterns in alveolar macrophages. - Understand the use of the ImmuPHAGE™ and ImmuLUNG™ models in conducting customized evaluations focused on inhalation safety.

Next-Generation Safety Assessment Tools for Advancing In Vivo to In Vitro Tra...

InsideScientific

In this webinar, Aditya Pratapa and Lorcan Sherry present a new workflow for analyzing multiplex immunoflurescence images. Spatial Signatures are a new class of highly predictive biomarkers that measure the interactions and cellular densities of tumor and immune cells that compose the tumor microenvironment. Based on multiplex immunofluorescence, spatial signatures provide a deeper understanding of complex interactions between tumors and the immune system, enabling improved patient stratification for immunotherapies. A significant hurdle to date has been in developing a data analysis workflow that is straightforward and user-friendly to transform the data rich images into meaningful quantitative spatial signatures. In this webinar, Aditya and Lorcan review the key features of the new PhenoImager HT 2.0 data analysis workflow. This workflow introduces a simplified framework from scanning to analyzing spectrally unmixed multiplex immunofluorescence images generated on the PhenoImager HT platform. The ready-to-analyze data can be directly imported into image analysis software such as Visiopharm. This presentation covers key aspects of data analysis elements such as image QC, segmentation, phenotyping, and verification – all essential for creating outputs that support the development of a spatial signature. Key Topics Include: - Understand Akoya’s new HT 2.0 data analysis workflow - The challenges in multiplex immunofluorescence analysis and the use of AI and cell lineage segmentation considerations - Explore OracleBio’s image analysis workflow incorporating Visiopharm - Evaluation of analysis data to facilitate spatial profiling and interpretation

A Ready-to-Analyze High-Plex Spatial Signature Development Workflow for Cance...

InsideScientific

In this webinar, Dr. Sabine Kuss will discuss the importance of transmembrane molecule exchange and how to detect and quantify membrane transport of molecules in cells. Complex biological processes, such as the transport of molecules across cell membranes, are difficult to understand using purely biological methodologies. Investigating cellular transport processes is challenging, because of the highly complex chemical composition of cells and the diffusion of molecules in and around cells at low concentrations. The development and advancement of electroanalytical methods over the last two decades has enabled the monitoring of living cells and their interaction with the environment, including external stimuli, such as pharma-molecules. This presentation emphasizes electrochemical and electrophysiological methods of detection and quantification but also makes a comparison to other bioanalytical approaches. Join us to discover a substantial diversity in methods used to monitor the transport of cell metabolites, crucial for cell survival, and pharmaceutical compounds, involved in cell characteristics such as drug resistance. Key Topics Include: - Understanding transmembrane molecule transport through bioanalytical methods - Electrochemical approaches to monitor molecule transport across cell membranes - What bioanalytical and especially electrochemical approaches can reveal - Challenges associated with instrument limitations

Molecule Transport across Cell Membranes: Electrochemical Quantification at t...

InsideScientific

In this webinar, Dr. Victor Arrieta highlights the link between p-ERK activation and improved survival in rGBM patients using anti-PD-1 immunotherapy. Recurrent glioblastoma (rGBM) has displayed a varied response to anti-PD-1 immunotherapy, necessitating the identification of predictive biomarkers. Through extensive analyses and 3 clinical studies, we have identified that activation of the MAPK/ERK signaling pathway, particularly ERK1/2 phosphorylation (p-ERK), is associated with longer overall survival (OS) in rGBM patients receiving PD-1 blockade. Initially, enrichment of BRAF/PTPN11 mutations was reported in 30% of responsive rGBM patients, prompting the investigation of p-ERK as a potential marker beyond these mutations. Our research has unraveled an association between p-ERK abundance and better clinical outcomes following PD-1 blockade, with p-ERK mainly localized in tumor cells. Notably, high p-ERK GBMs contained unique microglia and macrophage phenotypes with elevated MHC class II expression, suggesting a novel interplay between MAPK activation and the tumor immune microenvironment. While these insights establish a pivotal role for p-ERK in predicting PD-1 blockade response in rGBM, the implementation in clinical settings calls for further validation and accuracy. Nonetheless, these findings pave the way for more personalized and effective immunotherapy strategies, emphasizing the significance of the tumor microenvironment and its interaction with therapeutic interventions in GBM. Key Topics Include: - The activation of the MAPK signaling pathway, specifically ERK1/2 phosphorylation (p-ERK), is identified as a predictive biomarker for longer overall survival in recurrent glioblastoma (eGBM) patients undergoing PD-1 blockade - High p-ERK tumors in rGBM present a distinct myeloid cell phenotype with elevated MHC class II expression, signifying a connection between MAPK pathway activation and the immune microenvironment - The implementation of p-ERK as a predictive biomarker in clinical settings requires further validation and exploration of variables impacting its evaluation

Exploring Predictive Biomarkers and ERK1/2 Phosphorylation: A New Horizon in ...

InsideScientific

Dr. Reilly Enos and Dr. Eran Levin discuss estrogen's metabolic impact and how isotopic labeling and 13C-labeled nutrients can be used for animal physiology and nutrition research. Reilly Enos, PhD – Harnessing the power of estrogen to regulate metabolic processes Dr. Reilly Enos’ research focuses on the role that sex steroids and their receptors play in regulating metabolic processes, particularly in the setting of obesity. In this webinar, Dr. Enos will discuss his research on tissue-specific fluctuations of sex steroids throughout the estrous cycle in mice, provide insights into the importance of the quantity of estrogen necessary to impact physiological processes, as well as an understanding of the central versus peripheral effects of estrogen action. Eran Levin, PhD – Unlocking Insights: Utilizing 13C Labeled Nutrients for Cutting-Edge Physiology and Nutrition Research Dr. Eran Levin will discuss the potential of using 13C-labeled nutrients in physiology and nutrition research in animal models. Specifically, he will share practical tips for designing and conducting experiments using isotopic labeling techniques and demonstrate how they can provide unprecedented insights into metabolic pathways, nutrient utilization, and behaviors in both vertebrate and invertebrate models including insects, reptiles, and mammals. Key Topics Include: - The role that estrogen plays in regulating metabolic and behavioral processes in males and females - The tissue-specific fluctuations of sex steroids throughout the estrous cycle - Insight into the importance of tissue-specificity in developing hormonal therapies - The importance of estrogen quantity in regulating physiological processes - Understand the diverse range of 13C labeled nutrients available - Specific applications of labeled amino acids in studies of protein metabolism, cellular signaling, and typical nutrient utilization - How to integrate 13C labeling techniques with respirometry for a comprehensive assessment of metabolic processes, energy expenditure, and substrate utilization in animal models - How to calculate metabolic rates in free-flying animals using 13C bicarbonate

Exploring Estrogen’s Role in Metabolism and the Use of 13C-Labeled Nutrients ...

InsideScientific

Experts present a cell-free plasma biobank and describe the role of longitudinal plasma samples for cancer research, disease monitoring, and biomarker development. Through liquid biopsies, it is now possible to repeatedly and non-invasively interrogate the molecular landscape of solid tumors via a blood draw over the whole treatment course. Until now, liquid biopsies can be used for screening, disease monitoring and prognosis. Circulating tumor DNA (ctDNA) and circulating tumor cells (CTCs) have been the most explored targets in this technology for commercial applications up to the present time. In collaboration with a continuously expanding oncology network, Indivumed Services has established a unique high-quality cell-free plasma biobank that is exclusively focused on collecting longitudinal whole blood samples from cancer patients. This allows molecular insight by providing quick access to longitudinal plasma from cancer patients that have undergone treatment. ctDNA can then be isolated from longitudinal cell-free plasma to allow for monitoring of disease progression by providing diagnostic and prognostic information, potentially in real time. Key Topics Include: - Gain insights into Indivumed Services’ longitudinal plasma collection process - Understand the advantages and benefits of utilizing longitudinal plasma samples for cancer research - Explore applications of longitudinal plasma samples for biomarker research and development of companion diagnostics

Longitudinal Plasma Samples: Paving the Way for Precision Oncology

InsideScientific

In this webinar, experts present a standardized stem cell line and its differentiation into neural cells for disease modeling and assay development. Reproducible research with human induced pluripotent stem cells (iPSCs) depends on thoroughly characterized and quality-controlled cell lines. In this webinar, Dr. Andrew Gaffney and Dr. Erin Knock from STEMCELL Technologies describe the generation of a standardized induced pluripotent stem cell (iPSC) line. Developed with the upcoming ISSCR Standards Initiative characterization guidelines in mind, this highly characterized line is karyotypically stable, demonstrates trilineage differentiation potential, and expresses undifferentiated cell markers. Further, STEMCELL has developed a highly pure, ready-to-use neural progenitor cell product expressing PAX6 and SOX1 over multiple passages. Dr. Knock shows how these multipotent cells are suitable for customized downstream differentiation to various CNS cell types, such as forebrain neurons, midbrain neurons, and astrocytes. These progenitor cells are the ideal controls for standardizing downstream differentiation protocols, modeling diseases, and assay development. Key Topics Include: - Discover how STEMCELL’s induced pluripotent stem cell lines are derived and characterized - Learn how to differentiate induced pluripotent stem cell lines into all three germ layers - Explore the features of STEMCELL’s neural progenitor cell product - Differentiate neural progenitor cells into a variety of neural cell types, including neurons and glia

Fully Characterized, Standardized Human Induced Pluripotent Stem Cell Line an...

InsideScientific

Ian Foster and Steven Loo-Yong-Kee discuss Artisan Bio's STAR-CRISPR system for optimized gene editing in cell therapy, with a focus on the genetifc modification of T cells for cancer immunotherapy. Cell therapy is an emerging field with great promise for the treatment of various diseases. One of the most exciting areas of cell therapy is the use of T cells that have been genetically modified to recognize and kill cancer cells. While the use of T cells for cancer immunotherapy has tremendous promise, there is still room for improvement. The efficiency, expansion, and functionality of T cells can be enhanced by genetic modification using the STAR-CRISPR system. Artisan Bio is a biotechnology company focused on developing a CRISPR-mediated editing platform to improve the efficacy and safety of cell therapy products. In this webinar, we will provide a comprehensive overview of Artisan Bio’s STAR-CRISPR system, which is designed to improve the specificity and efficiency of gene editing for cell therapies. We will explain the system’s key components and how we are using a risk-based approach to optimize and validate the editing platform. The webinar will focus on Artisan Bio’s approach to building T cell OS/APPS through iterative improvements to achieve best-in-class editing capabilities and improved cell health metrics. Key Topics Include: - Learn about Artisan Bio’s proprietary high-performance STAR-CRISPR system for improving the specificity and efficiency of gene editing for cell therapies - Explore Artisan Bio’s risk-based, systems approach to technology development, including how to implement Design of Experiments (DoE) and Quality by Design (QbD) principles to optimize and validate any process - Case study of the application of QbD to Artisan Bio’s STAR-CRISPR platform to edit T cells for cancer immunotherapy with preliminary data showing improved efficacy, expansion, and functionality

How to Create CRISPR-Edited T Cells More Efficiently for Tomorrow's Cell Ther...

InsideScientific

Dr. Bert Bond and Max Weston will present an overview on their study investigating the effects high-intensity interval exercise has on cerebrovascular health. Physical activity reduces the risk of developing cardiovascular diseases (CVD) and dementia. This benefit cannot be explained by changes in traditional CVD risk factors alone, and direct improvements in vascular health are thought to play a key role. However, our understanding of how exercise can be optimized for improvements in blood-vessel health is limited. High-intensity interval exercise (HIIE) is known to improve peripheral vascular function, and there is a growing interest in the effects of HIIE on cerebrovascular health. However, it is not clear whether the acute improvements in peripheral vascular function following HIIE are also seen in the major blood-vessels of the brain. In the Bond lab’s study, 30 minutes of HIIE completed at both 75% and 90% V̇O2max improved peripheral vascular function 1 and 3h following exercise in healthy young adults, compared with work-matched continuous moderate-intensity exercise and a sedentary control condition. By contrast, cerebrovascular function was unchanged following all conditions. This is the first study to identify that acute improvements in peripheral vascular function following high-intensity interval exercise are not mirrored by improvements in cerebrovascular function in healthy young adults.

Peripheral and Cerebral Vascular Responses Following High-Intensity Interval ...

InsideScientific

Dr. Sam Sternberg discusses a novel CRISPR-Cas9 system using programmable, RNA-guided transposase, and highlights its implications for kilobase-scale genome engineering in cell and gene therapies. The utility of programmable, RNA-guided CRISPR-Cas systems in genome engineering continues to evolve. Nature has afforded scientists novel and diverse gene editing functionality, from nuclease-dependent CRISPR-Cas9 to second-generation base and prime editors that do not produce double-strand breaks. In this webinar, Dr. Sam Sternberg describes a new CRISPR-Cas9 paradigm relying on nuclease-deficient bacterial transposons that catalyze RNA-guided integration of mobile genetic elements into the genome. The discovery of a fully programmable, RNA-guided transposase lays the foundation for kilobase-scale genome engineering with broad applications for developing cell and gene therapies. Key Topics Include: - The basics of first- and second-generation CRISPR-Cas technologies from a scientist at the forefront of their development - Mechanisms, accommodation, and cell type diversity of CRISPR-Cas programmable transposition - How transposase factor coordination enables highly specific, genome-wide DNA integration to target sites - Implications of programmable transposases that obviate the need for DNA double-strand breaks and homologous recombination

Leveraging Programmable CRISPR-Associated Transposases for Next-Generation Ge...

InsideScientific

Dr. Marcel Perret-Gentil presents six simple-to-implement techniques to significantly improve surgical outcomes. You may feel proficient, even confident in performing rodent surgery; however, you may be surprised how simple improvements can have a huge impact to your animal’s recovery and data. The presentation is designed for individuals who have minimal or no rodent surgical skills but is also a great opportunity for those with considerable experience wanting to improve outcomes as well as teach such key principles. Key Topics Include: - Improve surgical outcomes that will lessen post-op morbidity and mortality - Improve data yield after rodent surgery - Implementation of key principles into a rodent surgical program

Simple Tips to Significantly Improve Rodent Surgical Outcomes

InsideScientific

A world-wide spread of the novel Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has triggered a pandemic and generated over 600 million reported cases around the globe. A substantial portion of patients who suffered Corona Virus Disease 2019 (COVID-19) have not recovered completely but continue to experience lingering symptoms for months to years. This novel clinical syndrome has been termed Long COVID or Post-acute Sequalae of COVID-19 (PASC). Observational studies have indicated that in about one third of cases PASC can be associated with cardiovascular (CV) autonomic dysfunction including postural orthostatic tachycardia syndrome, inappropriate sinus tachycardia, orthostatic hypotension, reflex syncope and microvascular dysfunction. The presence of CV autonomic dysfunction in PASC is important to detect since although frequently overlooked, it may be effectively treated in contrast to many other Long COVID-related symptoms. This webinar highlights CV dysautonomia as a specific sequalae of acute COVID-19 and guides the audience in the diagnostic work-up of PASC patients with suspected cardiovascular complications.

Cardiovascular Autonomic Dysfunction in the Post-COVID Landscape: Detection a...

InsideScientific

Cell lines are the core of biological research. Scientists need cell lines for drug development, basic biology research, safety testing, and biologic therapeutic production. Since the 1980s, genetic manipulation has allowed researchers to tailor cell lines to the experiment or production purpose. Over time, the requirements for these cell lies have risen. In many cases, the cells require multiple genetic edits and must produce data that passes FDA. Moreover, the current funding environment often requires rapid delivery of these cells so scientists can produce data to support further budget and/or investment. This is particularly acute for knock-in cell lines. Current technologies may take months to complete a cell line, allow a limited number of edits, and often have off-target effects that are not suitable for FDA filings. ExpressCells uses its patented FAST-HDR plasmid--along with CRISPR, to address these problems. The FAST-HDR process can precisely knock-in multiple genes (while supporting other types of genetic modifications), ensure precise placement of these edits, and deliver them months faster than competing technologies. This webinar will discuss the basis of the FAST-HDR technology and illustrate several uses. The first part is a presentation by Oscar Perez-Leal, MD, the inventor of the technology. Oscar will discuss the problems he faced as a researcher and how FAST-HDR was designed to address them. He will outline the details of the technology, the history of its development, and several examples where he used FAST-HDR. The second part is a conversation with Jon Weidanz, PhD. Jon will outline the challenges he faced at AbeXXa and how he selected a FAST-HDR custom cell line for his project. He'll outline the learnings from using this cell line, some of which were unexpected, but valuable to future development. By attending this program, attendees will: - Understand the current challenges in creating custom gene-edited cell lines - Know the technology underlying the FAST-HDR gene-editing system, including its use with CRISPR - Be able to describe the advantages of the FAST-HDR system - Learn about several case studies using gene-edited cell lines

Creating Better Gene-Edited Cell Lines with the FAST-HDR System

InsideScientific

Watch Dr. Sarah Greising discuss the current pathophysiologic understanding of the skeletal muscle remaining following traumatic musculoskeletal injuries. Volumetric muscle loss (VML) injuries result in the abrupt loss of skeletal muscle fibers, causing chronic functional disability in part due to limited muscle regeneration and vast co-morbidities. With a focus on clinically relevant outcome measurements for skeletal muscle function in both small and large animal models of VML injury, this webinar presents various near-term interventions for the restoration of tissue function following complex injuries. Interventions evaluated focus on regenerative rehabilitation approaches using regenerative pharmaceuticals to correct underlying muscle pathophysiology.

Functional Recovery of the Musculoskeletal System Following Injury - Leveragi...

InsideScientific

In this webinar, experts provide an overview of causal inference, along with step-by-step guidance to designing these studies using real-world healthcare data. Causal inference is used to answer cause and effect research questions and yield estimates of effect. Causal study design considerations and statistical methods address the effects of confounding variables and other potential biases and allow researchers to answer questions such as, “Does treatment A produce better patient outcomes compared to Treatment B?” Causal study interpretations have traditionally been restricted to randomized controlled trials; however, causal inference applied to observational healthcare data is growing in importance, driven by the need for generalizable and rapidly delivered real-world evidence to inform regulatory, payer, and patient/provider decision making. The application of causal inference methods leads to stronger and more powerful evidence. When these techniques are applied to observational data, the results generated are both from and for the real world. Presenters walk through several real-world case studies including the PCORI-funded BESTMED study and a collaborative study with a prominent pharmacy payer.

Designing Causal Inference Studies Using Real-World Data

InsideScientific

Many new data are emerging in recent years - real time data is collected through digital health technologies, including apps and wearables, monitoring data, social media data, public datasets, and patient organization data, in addition to primary and secondary datasets. Real life data are highly informative and can be used to address a range of challenges throughout the product life cycle. Data from social media can generate valuable insights as patients often gather in digital communities to get answers and share their experiences. Conversations on social networks merit special consideration as they can have real world influence over treatment management decisions. Social media data can reveal the motivations that impact patient healthcare decisions and behaviors through each stage of the care pathway. These data provide both the patient and caregiver perspectives at the same time. For this reason, conversations on social networks offer an opportunity to deepen our understanding on: - The fears and hopes associated with patient treatments - Daily needs and difficulties patients are facing in managing their disease - The impact of disease on patient health related quality of life - Identification in real life of the stages of the care pathway and patient perceptions - Reactions to health policies Watch this webinar for insights on how to collect, use, analyze, and interpret social media data in different contexts. Our experts share knowledge from over fifteen years of successfully developing and adapting algorithms to treat this kind of data.

Social Media Data: Opportunities and Insights for Clinical Research

InsideScientific

To learn more visit: https://insidescientific.com/webinar/we-are-more-than-what-we-eat-dietary-interventions-depend-on-sex-and-genetic-background/ Despite evidence that sex and genetic background are key factors in the response to diet, most studies of how diet regulates metabolic health and even longevity in mice examine only a single strain and sex. Using multiple strains and both male and female mice, Dr Lamming's team has found that improvements in metabolic health and in longevity in response to reduced levels of protein or specific amino acids strongly depend on sex and strain. While some phenotypes were conserved across strains and sexes, including increased glucose tolerance and energy expenditure, they observed high variability in adiposity, insulin sensitivity, and circulating hormones. Using a multi-omics approach, they identified mega-clusters of differentially expressed hepatic genes, metabolites, and lipids associated with each phenotype, gaining new insight into role of the energy balance hormone FG21 in the response to protein restriction.

We Are More Than What We Eat Dietary Interventions Depend on Sex and Genetic ...

InsideScientific

Amy Sheng, PhD provides an overview of antibody screening platforms and presents applications and case studies using the Beacon® platform for antibody discovery. Single B cell screening is a powerful and efficient strategy for generating antigen-specific monoclonal antibodies. Distinguished with fluorescence-activated B cell sorting, the Beacon® platform is based on plasma cell screening, making it easier to obtain antibody genes. The Beacon® single-cell optofluidic system combines a unique optoelectro positioning (OEP) technology with novel microfluidic technology. It can be used to accurately select single cells on a chip, perform multiple single-cell assays, and export target cells based on specific results. The Beacon® optofluidic platform preserves the diversity of B cells, generating high-quality positive hits at an early stage of discovery and avoiding the loss of “good clones”. Key Topics Include: - B cell differentiation and development - Pros and cons of mainstream antibody screening platforms - Mechanisms, applications, and case studies using the Beacon® platform for antibody screening - Sino Biological’s capacity using the Beacon® platform

Antibody Discovery by Single B Cell Screening on Beacon®

InsideScientific

Phil Griffiths, PhD, presents a summary of chronic cardiovascular telemetry studies and considerations for experimental design. Ensuring you collect the best and most physiologically accurate data from your chronic telemetry experiments requires careful planning and experimental design. This webinar will give an insight into the practical aspects of designing chronic animal experiments to set you on the best path for success. The benefits of chronic studies, how to select the most appropriate sample size for your study, some basic tips and tricks for data acquisition and handling, and how to ensure high animal welfare are discussed. Key Topics Include: - What are the benefits of chronic over acute studies? - How to decide the best sample sizes and the length of experiments? - Basic tips for data acquisition and handling - How to maintain high animal welfare standards

Experimental Design Considerations to Optimize Chronic Cardiovascular Telemet...

InsideScientific

In this webinar, Dr. Dennis Turner delves into dementia syndrome, the metabolic changes that occur, and the importance of proper physiological monitoring of animal models. Brain metabolism transforms with normal aging, and transient, dynamic metabolic insufficiency may underlie critical progression from aging into dementia syndrome and Alzheimer’s disease (AD). Age-related brain metabolism balances vascular-related substrate supply and transport mechanisms into extracellular space to neurons with cellular metabolic needs and utilization. Dynamic metabolic insufficiency can occur when there is intermittent supply-demand mismatch. Adequacy of neurovascular coupling to provide sufficient cerebral blood flow (CBF) to meet neuronal demand in vivo in a mouse AD model, compared to aged controls were studied. Dr. Turner’s lab analyzed the response to maximal neuronal metabolic demands, spreading depression and anoxia, using imaging, CBF measurements, and oxygen and glucose levels. These in vivo studies require human-similar anesthesia conditions, through monitoring temperature, blood pressure/pulse oximetry, and respiration, to maintain homeostasis. The lab confirmed abnormal neurovascular coupling in a mouse model of AD in response to these metabolic challenges, showing disruption much earlier in dementia than in equivalently aged individuals. Chronic metabolic treatments could influence dementia syndrome progression.

Strategic Approaches to Age-Related Metabolic Insufficiency and Transition in...

InsideScientific

More from InsideScientific (20)