diagramatic explanation of glycolysis

1. Glycolysis

3.  Requirement of glucose to all tissues
 Occurs in cytosol
 Unique: occurs aerobically and anaerobically
 Depends of availability of oxygen and ETC

4.  Greek glykys: “sweet,”and lysis: “splitting”
 EM pathway (Gustav Embden and Otto(Gustav Embden and Otto
Meyerhof)Meyerhof)
 All carbohydrates to be catabolized must enter
the glycolytic pathway. Glycolysis is centralcentral in
generating both energy and metabolic
intermediaries.
 Takes place in all cells of body , Cytosol., Cytosol.
 Major energy generating pathway in tissues

6. Mg++
Mg++

7. Mg++
K+,
Mg++

9.  Formation of lactate allows the
regeneration of NAD+
,
glyceraldehyde-3-phosphate
dehydrogenase, glycolysis proceeds
even in the absence of oxygen to
supply ATP.
• Fate of pyruvate depends on oxygen presence
• Anaerobic, pyruvate is reduced by NADH to lactate; LDH.
The occurrence of uninterrupted glycolysis is very essential in
skeletal muscle during strenuous exercise where oxygen
supply is very limited.

11. Characteristics Hexokinase Glucokinase
Tissue distribution: Most tissues Liver and β cells of
Pancreas
Km Low (0.05 mM/L) High (10 mM/L)
Vmax Low High
Inhibition by G6P Yes No
Inducible No Inducible(the amount
present in the liver is
controlled by insulin)
Clinical significance Deficiency causes
hemolytic anemia
Patients with diabetes
mellitus show less activity
Biological Significance Involved in maintaining
intracellular glucose
concentration
Involved in maintaining
blood glucose
concentration
08/27/16Biochemistry for medics 11