2. Burnos mikroflora ir
probiotikų galimybės
Gitana Rėderienė, burnos higienistė
Profesionalios burnos higienos aktualijos 2018
Vilnius 2018 11 10
3.
4.
5.
6.
7.
8.
9.
10.
11.
12.
13.
14.
15. Bakterijų specifiškumas
Kai produkto sudėtyje nurodytas bakterijų kamienas, tai rodo,
kad produkto veikimas yra moksliškai ištirtas, o efektyvumas
įrodytas.
15
Probiotinė bakterija 1 Probiotinė bakterija 2
Grupė Pieno rūgšties bakterija Pieno rūgšties bakterija
Gentis Lactobacillus Lactobacillus
Rūšis Lactobacillus reuteri Lactobacillus rhamnosus
Kamienas L. reuteri Protectis L. rhamnosus GG
16. Bakterijų specifiškumas
16%Į
Probiotinė bakterija Auto
Grupė Lactic acid bacteria Vokiškas automobilis
Gentis Lactobacillus Volkswagen
Rūšis Lactobacillus reuteri VW Golf
Kamienas L. reuteri Protectis VW Golf 2.0 Turbo
VW Golf I ir VW Golf VI GTI, panašūs, bet ne tie patys automobiliai
Skirtinos šunų veislės pasižymi
skirtingomis charakteristikomis
16
17.
18. L. reuteri Prodentis gerai prisitvirtina
burnos ertmėje
• Bet kurios probiotinės bakterijos
turi pasižymėti gera išlikimo “galia”.
• Abi L. reuteri Prodentis padermės
gerai įsitvirtina burnos ertmėje
• Padermė L. reuteri ATCC PTA 5289,
išskirta iš žmogaus burnos ertmės,
turi didžiausią įsitvirtinimo
galimybę
0 2 4 6 8 10
L reuteri PTA5289
L reuteri DSM17938
L crispatus
L salivarus
L rhamnosus
L gasseri
Adhesion (ODxmm2)
SPECIALI BAKTERIJŲ KOMBINACIJA BURNAI
19. BioGaia Prodentis sudėtyje yra dvi
viena kitos veikimą papildančios
bakterijų rūšys
L. reuteri
DSM 17938
(motinos pienas)
L. reuteri
ATCC PTA 5289
(seilės)
+
Veikimas: stiprus reuterino producentas,
kuris inhibuoja burnos patogenus
Veikimas: sumažina uždegimą
blokuodamas TNF-α
1 2
0
100
200
300
400
500
600
700
Media ATCC55730 ATCC PTA5289
TNF-α(pg/mL)
0
5
10
15
20
25
30
35
ATCC55730 ATCC PTA5289
mMolreuterin/1012cells
DSM 17938 DSM 17938
Probiotic Lactobacillus reuteri biofilms produce antimicrobial and anti-inflammatory factors
Sara E Jones1,3 and James Versalovic
BMC Microbiology 2009, 9:35 doi:10.1186/1471-2180-9-35
20. KADA VARTOTI PROBIOTIKUS
Probiotikai dantims ir dantenoms naudingi kontroliuojant:
• gingivitą
• periodontitą
• blogą burnos kvapą
• turint implantą/ų
• nėščiųjų ar paauglių gingivitą
• gingivitą dėl ortodontinio gydymo
• pacientams, sergantiems diabetu
• vartojant medikamentus, sukeliančius dantenų išvešėjimą
• norint atstatyti bakterijų pusiausvyrą burnoje
• po gydymo antibiotikais
• po chemoterapijos kurso
• grybelio augimą, ypač senyvo amžiaus pacientams
21. METAANALIZĖS
• Expert Review of Anti-infective Therapy, Volume 14, Issue
7, 2016
• ”..oral administration of probiotics improved the recognized clinical signs of chronic and
aggressive periodontitis such as probing pocket depth, bleeding on probing, and
attachment loss, with a concomitant reduction in the levels of major periodontal
pathogens”
• Journal of Periodontology 11 March 2016
• In a published systematic review of probiotics as adjunct to standard treatment of
periodontitis the proof for Lactobacillus reuteri Prodentis was confirmed, as it was shown
to be the only probiotic effective in patients with chronic periodontitis. According to the
authors the results are similar to those of other adjuncts to treatment of periodontitis, such
as systemic antibiotics.
Bacteria is organized according to a taxonomic rank. Examples of taxonomic ranks are: group, genus, species and strain.
It is generally accepted in the scientific community that probiotics need to be defined on strain level in order to make specific claims.
References and articles:
The minimum criteria to be met for probiotic products are that the probiotic must be specified by genus and strain. Research on specific probiotic strains cannot be applied to just any product marketed as a probiotic.
WGO (World Gastroenterology Organisation) Practice Guidelines Probiotics and Prebiotics 1 May 2008
In 2004, ISAPP published a document with a consensus statement on how to use the term ’probiotic’. The aim of ISAPP is to provide more precise definition and applications for probiotic to be used as a guide for clinicians and consumers in differentiating the diverse products on the market. In their statement the emphasis importance of strain specificity.
ISAPP (International Scientific Association for Probiotics and Prebiotics) consensus statement on the scope and appropriate use of the term probiotic.
Hill, C. et al. Nat. Rev. Gastroenterol. Hepatol. advance online publication 10 June 2014; doi:10.1038/nrgastro.2014.66
You need to know the genus, species and strain of a particular probiotic in each product.
Nicole Blazek June 18, 2014
http://www.clinicaladvisor.com/not-all-probiotics-are-created-equal/article/356312/
Glossary:
Rank:
The relative position in a taxonomic hierarchy in biological classification. Examples of taxonomic ranks are group, genus, species and strain.
Taxonomy:
Science that covers description and identification, nomenclature and classification of organisms.
Classification of bacteria:
Based on certain shared properties, such as morphology (form), mode of fermentation and other biochemical characteristics, certain bacteria species are classified as belonging to the same genus.
Strain specificity:
The genus Lactobacillus is made up of around 150 recognised species, with a large variety of phenotypic, biochemical and physiological properties. Within every species there are several strains, isolated from different sources such as humans, plants or foods.
Research has shown that the effects of probiotics are strain-specific rather than species-specific.
An example from a different world:
Would you ever buy a car without specifying more than “I want a German car”? Or even “I want a VW Golf”?
Could someone convince you that VW Golf 1.5D and WV Golf 2.0 Turbo are the same car? There might be similarities, but do you expect the same output and performance from both cars?
Dog and/or iPhone example:
If you run a parallel to the microbial world, those are both L. reuteri but two different strains.
BioGaia internal data EC019
Probiotic Lactobacillus reuteri biofilms produce antimicrobial and anti-inflammatory factors Sara E Jones1,3 and James Versalovic
BMC Microbiology 2009, 9:35 doi:10.1186/1471-2180-9-35
