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1. To determine the overall chromosome map for E. coli, three Hfr strains ( #1 , 2 , & 3) were
obtained from F+ ancestors. The start times of transfer during conjugation matings with F strains
carrying auxotrophic, resistance, and fermentation markers were determined for each Hfr strain,
as listed. A. Using the times for transfer, draw a unified chromosome map of E. coli, showing the
order and relative distances among these markers. B. Place the origin of transfer (site of F
insertion) on the map for each Hfr strain. C. What is the total length of the E . coli genome, in
minutes of transfer time

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