1.Explain the difference between using (i) selective media and conditions and (ii) serial enrichment for isolating microorganisms for biotechnological applications. 2.What is the advantage of using isolation systems that do not select for microorganisms as compared to systems utilizing selective agents or processes? Explain the role of the following in culture-based strategies for isolating microbes for biotechnological applications: Medium, Incubation conditions, Selective or enrichment agents (including serial enrichment), Culturing technique. 3.Explain the following approaches of isolating rare microbes: a)Use of Diffusion chambers b)Dilution to infinity techniques .