Biology Internal Assessment - Check list 2022.docx
1. Preparedby:Mr. Emad Zeidan
The International Academy - Amman
M. Sc. MolecularGenetics
DP Biologymoderator
Title of the Internal Assessment
Research question:
Introduction:
You have to write two paragraphs.
The first paragraph: introduction about the topic/brief – enzymes in general and their uses / real-life application.
The second paragraph:
State and relate it to the studied topic (In DP Biology, we learned about enzymes in topic …..)
How and why did you become interested in the topic – use terms such as inspired, curious …
Hypothesis:
You can be specific about the highest temp or Ph
Or you can write: If the temp increase, the activity increases up to optimum temp, then it will start decreasing if the temp
exceeded 40.0 C, because …..
Scientific explanation / must be cited (intext citation – MLA 9):
Define enzyme (Depending on the chosen topic)
Explain enzyme features (Depending on the chosen topic)
Explain the mechanism of action of enzyme – key-lock & induced fit, (Depending on the chosen topic)
Explain factors affecting the activity of the enzymes (Depending on the chosen topic)
Provide one studyaboutthe effectof pH ortemperature (dependingonthe chosenIV of yourexperimental investigation) onthe
activity of catalase. (Depending on the chosen topic)
Variables:
IV: stated / range / unit /how its changed or measured
DV: stated / trials / unit /how its measured or calculated
CVs: stated / unit /how it will be controlled/explain the importance of controlling it considering how it’s going to affect the
reliability, accuracy, and validity of the experiment.
Materials:
5x300 ml beaker (± 2.00 ml) – you have to add the uncertainty of the instruments /devices /tools used
2. Method:
Steps
Started with command terms
Short and clear
Has IV / DV / CVs
Repeat steps ??? - ??? for another X trials
Repeat steps ??? - ??? using the rest of the IV (stated with the unit)
Safety,ethics,and environmental issues:
Safety:explainthe use of goggles,gloves,labcoat…. (mustbe relevanttothe usedchemicals)
Ethics:thinkaboutthe usedconcentrationsandvolume
Environmental issues:mustbe explained
(youhave to refertothe safetydatasheetforeach usedchemical:youcantype HCl + safetysheet)
All of the previousinformationmustbe cited(Intext citations –MLA9)
Resultsand discussions
Qualitative results –muchbetterto be in a table withtwocolumns.
Quantitative results –One table whichhasIV,trials,meanvalues,andstandarddeviation
(eachtable musthave:a title,correctheading,units,anduncertainty).
Graph: musthave:title,correctlabelingof axisincludingthe units,anduncertainty
Statingthe formulaof the meanfollowingbyone calculatedexample
Statingthe formulaof the standard deviation(STDV) /noneedtoprovide anexample.
You can use Excel sheet/scientificcalculatorforthe calculationof the meanandSTDV.
Discussionof the results:
- You mustdescribe the resultsbyreferringtothe qualitative data(youcanwrite:Accordingtotable 1, whichshowedthe ….)
- You mustdescribe the resultsbyreferringtothe quantitative data:
* You can write:Accordingtotable 2, whichshowedthe ….
* You mustcompare the meanvaluesandnot the trialsaccordingto the usedIV.
* State if there are any outliers/anomaliesandspecifythemwithunits.
- You mustdescribe the resultsbyreferringtothe graph:
* Statingthe trend/ pattern/ positive relationship/negativerelationship.
* The relationshipmustbe described(asthe tempincreasesthenthe ….decreases)
* State the maximumactivityandminimumactivityandatwhichrange of IV was happening.
- Commentonthe valuesof meansandthe standarddeviation(https://www.biologyforlife.com/interpreting-error-bars.html),
t-test(orany statistical test) consideringthe calculatedt-value andcritical t-value(degree of freedomandP=0.05),andR2
–
onlyforthe graphand not bar graph (coefficientof determination –R2
).
3. Conclusion:
- Must restate the hypothesis.
- State if you accept/rejectthe hypothesis(if it’snull oralternative).
- Explainhowthe acceptedhypothesisissupportedbythe qualitative results(the observedresultsmustbe explained).
- Explainhowthe acceptedhypothesisissupportedbythe quantitativeresults(meanresultsinthe table mustbe justifiedand
fullyexplainedbyreferringtothe scientificexplanationandpreviousstudiescarriedoutbya researcherandsupportingyour
hypothesis).
- The meanvalues,standarddeviation(errorbars as a resultof anomalies/outliers),t-test(oranystatistical test),andR2
– only
for the graph andnot bar graph (coefficientof determination –R2
) mustbe explained.
Evaluation:
Evaluation:Youmustexplaineachpoint
To be more organized,add 4 strengthsas bulletpoints thatmustbe explained.
A table containingexplainedweaknessesandexplained improvements,all inthe same table/
1- Strength:
Must include 4 strengths.
Thinkaboutthe range of IV >5 strong(<5 weaknesses)
A numberof trials.>5 strong(<5 weaknesses)
Instrumentanditsaccuracy in measuringthe DV
Didyou control all of the CVs?If yes,thenuse it as a strength
Commentonthe error bars / t-test(or any statistical test)/ R2
/ if theyapprovedthe relationship(yes –strength/no -
weaknesses)
2- Weaknesses:(4weaknessesandimprovementinone table)
Thinkaboutthe previousstrength.
Linkedthe measuredresultswithanypossibleerrors(randominthe takentrialsorsystematicinthe usedinstruments).
Anyanomalies/outliers.
3- Improvement:
Must be forthe previouslymentionedweaknesses.
Extension:
NewIV (itcouldbe one of the possible factorsaffectingthe studiedprocesses) +range withthe unit.
Newresearchquestion:completewithrange andunit+ time
Newhypothesis
NewDV;howit will be measured+unit
NewCVs
State CVSwitha brief abouthowitwill be controlled