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pH temperature compensation
1.
pH measurement By: Mohamed
Shaltout Phone : 01270000612 By Dr Mohamed Shaltout 01270000612
2.
Nernst Equation • ال قياس pH هو potentiometric
measurement معادلة القياس في يتبع Nernst By Dr Mohamed Shaltout 01270000612
3.
Theoretical Slope • معادلة Nernst هي معادلة خطية Y =
a + b X • و هي تبين العالقة فيما بين potential E (mV), pH and temperature (K) • من المعادلة الخطبة a هي ال intercept (offset) في معادلة Nernst ال offset هو E°(T) ال offset او ال intercept هي pH 7 • من المعادلة الخطية الميل slope هو b في معادلة Nernst ال Slope هو • بالتعويض عن القيم الثابتة ال slope يكافئ By Dr Mohamed Shaltout 01270000612
4.
Theoretical Slope • عند 25 ال مئوية
درجة slope المثالي (theoretical ) يكافئ • ال slope التالي الجدول في كما مختلفة حرارة درجات عند المثالي By Dr Mohamed Shaltout 01270000612
5.
Theoretical Slope By Dr
Mohamed Shaltout 01270000612
6.
Measured Slope ال لحساب slope عند
المعايرة تمت لو الجهاز لمعايرة الفعلي 25 مئوية درجة • نقوم بمعايرة الجهاز ب buffers 2 بحيث تقع قياسات العينات الفعلية فيما بين ال buffers 2 و ليكن (pH 4.00 and pH 7.00) • نقوم بتسجيل الجهد ال potential (mv) لكل buffer و ليكن للبفر 4.00 هو mV 173 و للبفر 7.00 هو -3.1 mV • نطبق في المعادلة التالية • يكون الميل slope الفعلي المحسوب هو • الميل في األصل كما واضح من الرسم و المعادلة هو سالب اإلشارة • لذا تكون القيمة – 58.89 By Dr Mohamed Shaltout 01270000612
7.
Measured Slope • الميل المثالي theoretical كما هو مذكور في الجدول عند 25 درجة مئوية هو -59.16 و الميل الفعلي calculated هو القيمة - 58.89 • بعض األجهزة تقوم بحساب ال slope كنسبة مئوية و ليس كقيمة مطلقة 𝐒𝐥𝐨𝐩𝐞 %
= 𝒄𝒂𝒍𝒄𝒖𝒍𝒂𝒕𝒆𝒅 𝑺𝒍𝒐𝒑𝒆 𝒕𝒉𝒆𝒐𝒓𝒊𝒕𝒊𝒄𝒂𝒍 𝒔𝒍𝒐𝒑𝒆 𝑿𝟏𝟎𝟎= 99.5% By Dr Mohamed Shaltout 01270000612
8.
Calibration Evaluation ال جهاز
معايرة تقييم pH • ال لجهاز مصنعة شركة كل لمانول طبقا pH ال من كال تخص للمعايرة رفض و قبول حدود هناك slope ال و offset الجهاز معايرة عند الفعلي التالية الحدود نتبع يوجد لم ان و : • Slope should be 58± 3 or from 93% : 103% as percentage • Offset ( potential at pH 7) should be ± 30 mv By Dr Mohamed Shaltout 01270000612
9.
Automatic temperature compensation
(ATC) • مما سبق كما هو واضح من معادلة Nernst درجة الحرارة معامل مؤثر في قياس ال pH لذا يجب ان يتم قياس العينات الفعلية في نفس درجة حرارة ال buffers المستخدمة في المعايرة • لكن ذلك صعب تطبيقه على ارض الواقع • لذا نلجاء الى التعويض عن درجة الحرارة Automatic temperature compensation (ATC) ذلك يتم فكيف ,,,,,,, By Dr Mohamed Shaltout 01270000612
10.
Automatic temperature compensation
(ATC) • الميل Slope سوف يتم تعديله الن درجة حرارة العينات الفعلية اختلفت عند درجة حرارة ا ل buffers التي استخدمت في المعايرة By Dr Mohamed Shaltout 01270000612
11.
Automatic temperature compensation
(ATC) عملي مثال • عند معايرة جهاز pH باستخدام 2 بفر 4.00 and 7.00 عند درجة حرارة 24°C كان الميل المحسوب هو slope (24°C) = 58,0 و ال offset = 0.0 mV • و عند قياس العينات الفعلية كانت درجة حرارة العينات هي 10°C. • لعمل Automatic temperature compensation (ATC) نطبق في القانون By Dr Mohamed Shaltout 01270000612
12.
Automatic temperature compensation
(ATC) • Slope(10°C) = slope(24°C) * (10 + 273.15) / (24 + 273.15) • Slope(10°C) = 58.0 * (283.15) / ( 297.15) • Slope(10°C) = 55.28 • لو كان قياس الجهد potential للعينة هو 100 mv • نعوض في معادلة Nernst By Dr Mohamed Shaltout 01270000612
13.
Automatic temperature compensation
(ATC) pH = 7 – Sample potential (mv)/ Slope • قياس ال pH للعينة لو لم يكن هناك تعويض عن االختالف في درجة الحرارة بين البفر و العينة = 7 – (100 mV / 58.0) = pH 5.28 (not corrected) • قياس ال pH للعينة عندما يكون هناك تعويض عن االختالف في درجة الحرارة بين البفر و العينة = 7 – (100 mV / 55.28 mV/pH) = pH 5.19 (corrected) By Dr Mohamed Shaltout 01270000612
14.
جزيال شكرا By Dr
Mohamed Shaltout 01270000612
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