1. Diana Marcela Grecco Herrera
Medicine student

4. * ACT is a control of high precision
RNA synthesis, a reliable copy
depends on it. Is an intern
change of RNA polymerase.
*B cell lymphoma is a Non-
Hodgkin lymphoma, is a
heterogeneous group of
lymphoproliferative
malignancies.

5. * E. Sosunova, V. Sosunov, et
al. Control of transcription
fidelity by active center tuning as
derived from RNA polymerase
endonuclease reaction. Journal of
Biological Chemistry, 2013;
DOI: 10.1074/jbc.M112.424002

6. * In RNA polymerase the
functionally state of the ACT
works with two Mg ions
coordinated by an aspartate
triad
* ACT turn on when: you have to
correct NTP substrates or you
have free RNA residues. (Need
an internal change)

7. * Transcript scission Gre factors provides an ACT
stabilization to Mg ions and strongly reinforce
the proofreading.
* The mechanism of DNA polymerase has 2
different active centers sites to synthesis and
proofreading. (Doesn´t need an internal
change)

8. DNA Multisubunit RNA
polymerase polymerase have
active center a flexible loop.
are ACT has an
carboxylates important re-
stem from rigid shaping of the
scaffolds loop.

9. * I think that it is important to investigate the
process that can influence in an replication or
the transcription of the DNA, because if we
find out the process in which it went wrong,
we can make a treatment specially designed to
repair or even heal the DNA malfunction. The
first step is to investigate the process, as the
researchers made here.

10. * Jacqueline H. Barlow, Robert B.
Faryabi, et al. Identification of
Early Replicating Fragile Sites
that Contribute to Genome
Instability. Cell, 2013;
DOI:10.1016/j.cell.2013.01.006

11. * The B lymphocytes are among the most rapidly
dividing cells resulting in a risk of replication-
induced DNA damage.
* Double-strand breaks at fragile sites as
repetitive sequences that are hard to copy,
this happen when DNA is replicated prior to
cell division.

12. * B-cell have the activation-induced cytidine
deaminase (AID) which causes collateral
damage in the genome in the non-replicative
stage of the cycle.

13. * AID-independent source of DNA breaks that is
associated with replication which is also called
an early replicating fragile sites (ERFSs).
* DNA damage occurs at ERFSs in thee early
stages of replication.
ERFSs overlapped with DNA
alterations associated with B cell
lymphoma

14. * Locate ERFs as possible point of the beginning
of the B cell lymphoma, which can impulse the
investigation of cancer cures to this kind or
ways to handle the sickness. Furthermore it
can give clues to work with other kind of
cancer.

15. * By investigating we can change
the current diagnostic and
treatment of genetic
malfunctions, by giving
opportunities to controling the,
as a molecular level, as the
processes of these cell.
study the mechanism
of control of
replication and
transcription

16. * The "ACT" gives the investigators a molecular
blank to avoid the DNA damage.
Tool that let
you control
the fidelity of
the ACT
replication

17. * Findout how B cell lymphoma acts and start
helping in the therapeutic area  Giving basis
in developing drugs.

18. * The replication-induced DNA damage could be
used by many kind cancer to arise.
The results of the study can be
used to investigate different
kinds of cancer.

19. * · [http://www.sciencedaily.com/releases/2013/01
/130115111109.htm] checked on February 4th of
2013. “ New Paths Explored for Curbing Genetic
Malfunctions”
* · [http://www.sciencedaily.com/releases/2013/01
/130124123307.htm ] checked on February 5th of
2013. “Discovery of New Class of Damage-Prone
DNA Regions Could Lead to Better Cancer
Treatments”
* · [http://emedicine.medscape.com/article/202677
-overview] Checked on February 8th of 2013. “B-
cell Lymphoma”

20. “Some people want it to happen, some wish it
would happen, others make it happen.” M.
Jordan.