Aspectos generales sobre el articulo Real-time loop-mediated isothermal amplification assay for rapid detection of human papillomavirus 16 in oral squamous cell carcinoma

1. Daniel Sierra Castillo y Ana María Sierra Gomez
Tercer semestre Medicina
Universidad Pontificia bolivariana

2. Papilloma virus 16
It is a small cyclic
DNA virus with a
naked shell
Does not encode
polymerase, it depends
on the transcription and
replication machinery of
the host cell.
It infects the basal layer
of the squamous
epithelium and becomes
internalized and denuded,
delivering the DNA to
the nucleus
It causes lesions on the
vulva, cervix and penis.
especially he can
progress to carcinoma.
Its proteins E6 and E7 are combined with the p53 protein and
the p105RB protein, which cancels out the cell regulation cycle.

3. Carcinoma
escamo cellular
Is the most
common
malignancy in
the oral cavity.
Location:
Lower lip, the side
edges of the
tongue and the
floor of the
mouth.
Characteristics
White plates,
erythematous plates,
plates between
erythematous and
white, and raised-edge
ulcers
Squamous cells have
eosinophilic cytoplasm,
hyperchromatic nucleus
and an increase in the
relation nucleus
cytoplasm

4. “Objective: The present study
established a real-time loop-mediated
isothermal amplification (qLAMP)
for rapid detection of human
papillomavirus subtype 16 (HPV-16)
in oral squamous cell carcinoma
(OSCC).”

5. Detection of
high-risk HPV
by nested PCR
Se trata de una variante de la PCR
básica que utiliza dos pares de
cebadores. Se utiliza para amplificar
fragmentos muy específicos del
genoma.
Se utilizo para la
detección de VPH de
alto riesgo.

6. Detection of β-globin
gene by PCR
En este caso identificar
si el papiloma virus se
encontraba presente en
ese tipo de cáncer oral
Se usa para hacer la
amplificación específica
de una secuencia de
material genético.

7. p16 immunohistochemistry
staining
Técnica utilizada para determinar la
presencia y el nivel específico de proteínas
celulares. Es un marcador cancerígeno.
Se utilizó para la identificación de la
proteína p16 en el tejido.

8. Se obtiene el ADN a partir de material biológico
(carrillo, saliva, sangre…) consiste en la
separación y purificación del ADN con
el fin de poder estudiarlo, analizarlo o
manipularlo.
En este artículo se hizo con el fin de
extraer y separar el ADN para poder
trabajar en él y seguidamente hacer los
demás estudios necesarios en el artículo
como la PCR
DNA
extraction

13. DISCUSIÓN
AUTHOR WHAT THEY SAID ACCORDING OR AGAINST?
Chai et al.
The overexpression of p16
was used as a surrogate
marker for detection of high-
risk HPV associated with
OSCC.
Mazurek, Rut- kowski,
Fiszer-Kierzkowska,
Malusecka y Sk ł adowsk
Reported 14% HPV positivity
in the blood samples of OSCC.
Chen, Sun y Jiang
Systemic regulation of blood
where presence of neutrophils
and other white cells helps to
combat and clear the foreing
materials, contributed to low
changes in detection of HVP in
blood.

14. Conclusions
04
In addition, through the laboratory tests
provided by molecular biology and its most
accurate results, we could treat patients with
less invasive techniques.
02
06
From the article we can conclude that molecular
biology through laboratory tests and comparisons
between them can help us make more accurate
diagnoses, with much smaller margins of error..
01
To conclude, with the help of the most precise and
advanced laboratories, we could diagnose diseases
at an early stage and avoid false positives in
patients, which lead us to choose the wrong
treatments.
03