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 Root lesion nematode (Pratylenchus spp) is next to root knot
and cyst nematode in economic importance
(Castillo and Vovlas, 2007)
 Most species are specific to crops and threshold level varies
from 2-18 nematodes/cm3 soil
 Taxonomic separation is difficult as the genus is stenomorphic
INTRODUCTION
 Very similar in gross morphology and specific differences can
only be detected using high magnifications
 Anomalies arise from underestimation of intra specific
characters
Morphological and Molecular Characterization of
Pratylenchus Species Occurring at IARI farm, New Delhi
To isolate lesion nematodes from IARI farm, New Delhi and
their morphological and molecular characterization
To integrate morphological and molecular data for authentic
and sound identification
To develop a checklist and compendium of globally known
Pratylenchus species
OBJECTIVES
A. Collection of composite soil samples from different crops
B. Extraction of nematodes from soil samples
C. Isolation of Pratylenchus species and their processing
D. Morphological and morphometric studies of specimens
E. Molecular studies based on ITS region of rDNA
F. Analysis and interpretation of data and thesis writing
Morphological and molecular characterization of
Pratylenchus species occurring at IARI farm, New
Delhi with their re-description
Thesis seminar_final.pptx

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Thesis seminar_final.pptx

  • 1.  Root lesion nematode (Pratylenchus spp) is next to root knot and cyst nematode in economic importance (Castillo and Vovlas, 2007)  Most species are specific to crops and threshold level varies from 2-18 nematodes/cm3 soil  Taxonomic separation is difficult as the genus is stenomorphic INTRODUCTION  Very similar in gross morphology and specific differences can only be detected using high magnifications  Anomalies arise from underestimation of intra specific characters
  • 2. Morphological and Molecular Characterization of Pratylenchus Species Occurring at IARI farm, New Delhi
  • 3. To isolate lesion nematodes from IARI farm, New Delhi and their morphological and molecular characterization To integrate morphological and molecular data for authentic and sound identification To develop a checklist and compendium of globally known Pratylenchus species OBJECTIVES
  • 4. A. Collection of composite soil samples from different crops B. Extraction of nematodes from soil samples C. Isolation of Pratylenchus species and their processing D. Morphological and morphometric studies of specimens E. Molecular studies based on ITS region of rDNA F. Analysis and interpretation of data and thesis writing
  • 5. Morphological and molecular characterization of Pratylenchus species occurring at IARI farm, New Delhi with their re-description