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8. Why do the wells change color when the chromogen is added? 9. Why are some positive
results lighter in color than others (what is there less of)? 10. Sometimes a person who has been
infected with the disease-causing agent will not test positive in an ELISA assay. Give two
reasons why this might happen (assume that your ELISA assay is working properly). 11. Why
was this assay performed in triplicate?

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