- Ashok Kumar Rout is a Senior Research Fellow at the Laboratory of Structural Biophysics at the National Institutes of Health. - He received his Ph.D. in Chemical Science from the Tata Institute of Fundamental Research in Mumbai, India. - His research focuses on using NMR spectroscopy to determine the structures of proteins, protein complexes, and membrane proteins to gain insights into cellular processes like transport mechanisms. - He has authored or co-authored over 10 publications in peer-reviewed journals and is currently working on several manuscripts.

1. Ashok Kumar Rout, Ph. D.
CURRICULUM VITAE
ASHOK KUMAR ROUT, Ph. D.
Laboratory of Structural Biophysics
Bldg. 50, #3505
National Institutes of Health (NIH),
Bethesda, MD 20892, USA.
Phone: +1-2402745488(M)
+1-3014514937(O)
Email: ashok08@gmail.com or ashok.rout@nih.gov
EDUCATION
• Post-doctoral Fellow - (Laboratory of Structural Biophysics), 2011-2013
National Institutes of Health (NIH), Bethesda, MD, USA
Advisor: Prof. Nico Tjandra
• Visiting Fellow - (Department of Chemical Sciences), May-Aug2011
Tata Institute of Fundamental Research (TIFR), Mumbai, India
Advisor: Prof. K. V. R. Chary
• Tata Institute of Fundamental Research (TIFR), Mumbai, India 2011
Ph. D. in Chemical Science
Advisor: Prof. K. V. R. Chary
Title: “Structure Based Functional Proteomics: NMR Characterization of a
Calmodulin like protein from Entamoeba histolytica”
• Utkal University, Bhubaneswar, Odisha, India 2004
Master of Science - Physical Chemistry
First Div., 78% (aggregate)
• Utkal University, Bhubaneswar, Odisha, India 2002
Bachelor of Science – Chemistry, Physics and Mathematics
First Div. with Distinction, 66% (aggregate)
PROFESSIONAL EXPERIENCE
• Senior Research Fellow – (Laboratory of Structural Biophysics), 2014-onwards
National Institutes of Health (NIH), Bethesda, MD, USA
Advisor: Prof. Nico Tjandra.
PUBLICATIONS:
1. Rout, A. K., Strub MP, Piszczek G, and Tjandra N., “Structure of Transmembrane Domain of Lysosomal-associated
Membrane Protein Type 2a (LAMP-2A) Reveals Key Features for Substrate Specificity in Chaperone Mediated Autophagy.”
J Biol Chem., 2014, Dec 19, 289(51): 35111-23. (IF=5.67)
http://www.ncbi.nlm.nih.gov/pubmed/25342746
2. Rout, A. K., Patel, S., Somlata, Shukla, M., Saraswathi, D., Bhattacharya, A., and Kandala V. R. Chary., “Functional
manipulation of a calcium binding protein from E. histolytica guided by paramagnetic NMR.” J Biol Chem., 2013, Aug 9;
288(32):23473-87. (IF=5.67)
http://www.ncbi.nlm.nih.gov/pubmed/23782698

2. Ashok Kumar Rout, Ph. D.
3. Rout, A. K., Padhan, N., Barnwal, R. P., Bhattacharya, A., Chary, K. V. R., “Calmodulin like protein from Entamoeba
histolytica: solution structure and calcium binding properties of a partially folded protein.” Biochemistry, 50(2), 2011, pp-
181-193. (IF=3.37)
http://www.ncbi.nlm.nih.gov/pubmed/21114322
4. Rout, A. K., Barnwal, R. P., Agarwal, G., Chary, K. V. R., “Root-mean-square-deviation based rapid backbone resonance
assignments in metal bound proteins in different states.” Magnetic Resonance in Chemistry, 48(10), 2010, pp. 793-797.
(IF=1.52)
http://www.ncbi.nlm.nih.gov/pubmed/20803498
5. Rout, A. K., R. Minda, D. Peri, S. K. Bhattachajee, B. J. Rao, and K. V. R. Chary. “Sequence specific 1
H, 13
C and 15
N
backbone resonance assignments of UVI31+ from Chlamydomonas reinhardtii.” Biomolecular NMR Assignments, 4, 2010,
pp. 171-174. (IF=0.63)
http://www.ncbi.nlm.nih.gov/pubmed/20526700
6. Rout, A. K., Barnwal, R. P., Padhan, N., Bhattacharya, A., and Chary, K. V. “Sequence specific 1
H, 13
C and 15
N resonance
assignments of a calmodulin-like calcium-binding protein from the protozoan parasite Entamoeba histolytica (EhCaM).”,
Biomolecular NMR Assignments (2008) 2, 77-79. (IF=0.63)
http://www.ncbi.nlm.nih.gov/pubmed/19636930
7. Barnwal, R. P., Rout, A. K., Chary, K. V. R., Atrya, H. S. “Rapid Measurement of Pseudo-contact shifts in Metallo-protein
using a suit of GFT-experiments.”, The Open Magnetic Resonance Journal, 1 2008, pp. 1-16.
http://www.benthamscience.com/open/tomrj/articles/V001/16TOMRJ.htm
8. Barnwal, R. P., Rout, A. K., Atrya, H. S., Chary, K. V. R., “Identification of C-terminal neighbours of amino acid residues
without an aliphatic 13Cγ
as an aid to NMR assignments in proteins.”, Journal of Biomolecular NMR, 41, 2008, pp. 191-7.
(IF=3.14)
http://www.ncbi.nlm.nih.gov/pubmed/18633715
9. Barnwal, R. P., Rout, A. K., Chary, K. V. R., Atrya, H. S. “Rapid measurement of 3
J(HN
Hα
) and 3
J(NHβ
) coupling constants
in polypeptides.”, journal of Biomolecular NMR, 39, 2007, pp. 259-263. (IF=3.14)
http://www.ncbi.nlm.nih.gov/pubmed/17914658
10. Ramakrishnan V., Barnwal P., Rout, A. K., H. S. Atreya, Kandala V. R. Chary, “Rapid Measurement of Structural
Parameters in Protein NMR.”, (Book Chapter in Future Directions in Nuclear Magnetic Resonance; Springer 2009, edited by
C. L. Khetrapal and Anil Kumar).
MANUSCRIPTS UNDER PREPARATION AND REVISION
1. Rout, A. K., Bermejo G. A., Strub M. P., Hammer J., and Tjandra, N., “Structural characterization of melanoregulin
revealed key features of the regulation of cellular pigmentation.”
(Manuscript under preparation)
2. Rout, A. K., Himanshu S., Patel S., R. Minda, B. J. Rao, and K. V. R. Chary. “NMR structural characterization of a putative
UV inducible protein (UVI31+) from Chlamydomonas reinhardtii that exhibits RNA and DNA endonuclease activity”
(Nucleic Acid Research, Under Revision)
3. Rout, A. K., Barnwal R. P., and K. V. R. Chary. “Rapid measurement of CH3-PCS in paramagnetic proteins using GFT
NMR.”
(under revision)
RESEARCH INTERESTS
• Spectroscopy and Structural Biophysics: Structural insights of peptides, proteins, protein-protein complexes, protein-DNA
complexes, and Organic and Inorganic molecules using solution state NMR spectroscopy.
• Membrane proteins: Structure and Functional characterization.

3. Ashok Kumar Rout, Ph. D.
• Exploring cellular transport mechanisms: (Chaperon Mediated Autophagy and LAMP2, Melanoregulin and Cellular
Pigmentation).
• New RDC and PRE probes for high resolution 3D structure determination of proteins, nucleic acids and their complexes.
• Molecular Biophysics: Using biophysical techniques such as LC-MS, DLS, CD, ITC, SPR, DSC, AUC, UV-Visible and
Fluorescence to understand biochemical and biophysical processes of biomolecules i.e., protein, nucleotides and their
complexes.
TEACHING/OTHER PROFESSIONAL EXPERIENCE
• Partly assisting students in their different projects in Prof. Tjandra’s Lab, NIH, USA (2011-present).
• Demonstrating and assisting to set up NMR experiments in Prof. Tjandra’s Lab, NIH, USA (2012-present).
• Partly supervised summer students and Ph.D. students of Prof. Chary’s Lab, TIFR, Mumbai, India (2008-2011).
• Actively involved in different scientific discussions and journal clubs at NIH and TIFR (2008-present).
TECHNICAL SKILLS
• NMR spectroscopy.
• Managing NMR facility of Prof. Tjandra’s Lab, NIH, USA (2012-present).
• Demonstrating and assisting to set up NMR experiments in Prof. Tjandra’s Lab, NIH, USA (2012-present).
• Managing and controlling different Lab activities of Prof. Tjandra’s Lab, NIH, USA (2014-present).
• 3D Structure determination of peptides, proteins, nucleotides, small molecules and large molecular complexes.
• Molecular interactions, active-site determination and further use of this information for potential drug design.
• Making and optimizing liquid crystals and stretched acrylamide gel for RDCs measurement.
• Measurement of Residual Dipolar Coupling (RDC), Pseudo-Contact Shift (PCS) and (Paramagnetic Relaxation Enhancement
(PRE) for NMR structure refinement.
• Liquid Chromatography-Mass Spectrometry (LC-MS), Dynamic Light Scattering (DLS), Analytical Ultra Centrifuge (AUC),
UV-Visible, Circular Dichroism (CD), Isothermal Titration Calorimetry (ITC), Surface Plasma Resonance (SPR),
Differential Scanning Calorimetry (DSC), Fluorescence.
• Ion Exchange and Gel Filtration Chromatography (FPLC, HPLC).
• Protein Engineering: Cloning, Protein Expression, Purification, Labeling, Western blotting, biological assays.
• Chemical Cross Linking and attaching different tags to proteins by chemical modification.
HONORS/AWARDS
• Received travel awards from Department of Health and Human Services, USA, for attaining the 56th
ENC at Asilomar,
California, USA, 2015.
• Received travel awards from Department of Health and Human Services, USA, for attaining the ICMRBS XXVI at Dallas,
Texas, USA, 2014.
• Received Ernst-Wuthrich award WWMR, Florence, Italy, 2010.
• Received Young Investigator award WWMR, Florence, Italy, 2010.
• Qualified Lectureship (LS)-University Grant Commission (UGC)-National Eligibility Test (CSIR-UGC-NET) in June 2005.
LECTURES AND PRESENTATIONS
• Poster presentation at 56th
ENC, Asilomar, California, USA, 2015.
• Oral presentation at MPIG-SBIG symposium, NIH, USA, 2015.
• Poster presentation at ICMRBS XXVI, Dallas, TX, USA, 2014.
• Workshop on Writing and Publishing a Scientific paper, NIH, USA, 2014.
• School on Academic Teaching and Job search, NIH, USA, 2014.
• School on “Hydrodynamic and Thermodynamic analysis of macromolecules with SEDFIT and SEDPHAT”, FAES, NIH,
USA, 2013.
• Poster presentation at WWMR, Florence, Italy, 2010.
• Oral presentation, Royal Society of Chemistry, Goa, India, 2009.
PROFESSIONAL SERVICES AND MEMBERSHIPS
• Life Membership of National Magnetic Resonance Society (NMRS), India.
• Life Membership of “TIFR Alumni Association”, TIFR, Mumbai, India.
• Member of “Alumni Association, Department of Chemistry, Utkal University”, Bhubaneswar, Odisha, India.

4. Ashok Kumar Rout, Ph. D.
• Volunteer for “Open Day”, TIFR, Mumbai, India.
• Volunteer for “Homi Bhabha Birth Centenary, TIFR, Mumbai, India, 2010.
REFERENCES
1. Prof. Nico Tjandra
Laboratory of Structural Biophysics, National Institutes of Health (NIH),
Bethesda, MD 20892, USA
Phone: +1 301 402 3029
Email: tjandran@nhlbi.nih.gov
2. Prof. Kandala VR Chary
Department of Chemical Sciences, Tata Institute of Fundamental Research (TIFR),
Colaba, Mumbai-400005, India
Phone: +91 22 22782489
E-mail: chary@tifr.res.in, chary54@gmail.com
3. Prof. H. S. Atreya
NMR Research Centre, Indian Institute of Science (IISC),
Bangalore-560012, India
Phone: +91 80 22933302
E-mail: hsatreya@sif.iisc.ernet.in
4. Prof. P. K. Madhu
Department of Chemical Sciences, Tata Institute of Fundamental Research (TIFR),
Colaba, Mumbai-400005, India
Phone: +91 22 22782874
E-mail: madhu@tifr.res.in
5. Dr. Marie-Paule Strub
Laboratory of Structural Biophysics, National Institutes of Health (NIH),
Bethesda, MD-20892, USA
Phone: +1 301 4514071
Email: marie-paule.strub@nih.gov
6. Prof. Alok Bhattacharya
School of Life Sciences, Jawaharlal Nehru University (JNU),
New Delhi, 110067, India
Phone: +91 11 26704516
Email: alok0200@mail.jnu.ac.in, alok.bhattacharya@gmail.com
Ashok Kumar Rout
Last updated: September 10, 2015