General Introduction and working of Probiotics in our body. The probiotics status of Nepal and Nepali food is Included here. Nepali food habits affects the digestive system.

2.  Introduction
 What are Probiotics
 Characteristics
 Modes of action
 Probiotics in Nepal
 Advantages and Disadvantages
 Probiotics as improviser.

3.  Probiotics are a combination of live beneficial
microbes that naturally live in our body.
 Probiotics generally is derived from Latin word
“Pro” and Greek word “biotics” which means
“for”&“Life”.
 The term was coined in 1965 by Lilly and
Stillwell.
Probiotis = For Life = LIFE PROMITING

4.  Probiotics:
› According to WHO/FAO
 “Live microorganism which when administered in
adequate amounts confer a health benefit on the host.”
› According to US FDA
 “Probiotics are referred to as live bio-therapeutic
products(LBP).”
 Probiotics = Healing disease using microbes

5.  Able to survive the passage through the digestive system.
 Able to attack to the intestinal epithelia and colonize.
 Able to maintain good viability.
 Able to utilize the nutrients and substrate in a normal
diet.
 Non-pathogenic, Non-toxic, anti-carcinogenic activity.
 Capable of exerting a beneficial effect on the host.
 It should be safe to host.
 It should produce lactic acid.

6. › Secrete bacterial proteins
› Decreases the pH
• Block the attachment and invasion o epithelium
by pathogens.
• Improve epithelial barrier function.
Increases mucus production
Increases integrity
 Stimulate IgA production
 Decreases proinlammatory cytokines
Produce β-galactosidase
Decreases production o carcinogenic metabolities.

8.  Use of ‘Dahi’ from the ancient historical era
is the best example.
 Preparation of different types of pickles.
 Different types of traditional foods.
 Immunity booster foods.
 Enhanced food therapies.(jamara ko juice)

10.  Antibiotic associated diarrhea
 Probotics and cancer
 Enhanced food products
 Treatment of high cholesterol.

12.  May cause digestive issues.
 Discomfort in the gastrointestinal tract
 Allergy
 Metabolic discomfort.

13.  Many fermented food are generally
considered as probiotic foods.
 The creative use of LAB in enhancing yogert,
meat cereals products.