Silver, gold and bimetallic nanoparticles production using single-cell protein (SCP).

1. Silver, gold and bimetallic
nanoparticles production
using single-cell protein
Biotechnology

2. Biological system:
Microorganism
(cyanobacteria)
Spirulina platensis

3. Substrate (Media)(Raw material):
Silver Nitrate
Ag No3
Gold(III) chloride solution
HAuCl4

4. Optimized conditions:
PH: 5.6 Temp: 37 °C Duration: 120 hrs.

5. Mechanisms of biosynthesis:

6.  The transport of the metal ions into the microbial cell wall.
 Electrostatic interactions between the negatively charged
cell wall and the positively charged metal ions.
 Enzymes residing within the cell wall reduce the toxic
metals to harmless nanoparticles.

7. Intracellular

8. Nitrate reductase
microbial enzyme
Hydroquinone-mediated
redox reactions

9. EXtracellular

10. Fermenter (Bioreactor):

11. Fermenter (Bioreactor):

12. Product:

13. Product detection:

14. Why do we follow this approach?
Chemical synthesis Biological Production
 Many disadvantages.
 Many limitations.
 Economic.
 No environmental hazards.
 Minimize health hazards.
 Green process.
 Fast, simple, easy.

15. Medical applications:
Antimicrobial Cytotoxic

16. Principal:
 A bottom-up approach.
 A part of a defense mechanism-based detoxification.
 A fundamental survival procedure involving;
of metal ions
of metal ions
 The bacteria become metal-resistant.
 Greater amounts of reduced metals accumulate, and more
nanoparticles being formed

17. Factors affecting nanoparticles formed:
 Nature of microorganism.
 Metal ion.
 The morphology, size and surface properties.
 Environmental conditions such as; pH, pressure, temperature.
 Fermentation process type
 Media sort.

18. Global approach:

19. Global approach:

20. Global approach:

23. Presented by:
Ahmed Abd El-Moniem Alsayed Amer
ID: 17037
Under supervision:
Prof. Dr. Eman Elgebaly

24. Thank You
O6U - Faculty of pharmacy – Biotechnology Department