16 s rdna based microbial identification f
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rdna based microbial identification f
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16 s rdna based microbial identification f
- 1. Microbial Identification using 16S rDNA based molecular method – Ethiraj College www.clinbiocaretechnology.com info@clinbiocaretechnology.com 1
- 2. Report of Microbial Identification Experimental Method 1. DNA was isolated from the culture provided by the scientist. 2. Fragment of 16S rDNA gene was amplified by PCR. 3. The PCR amplicon was purified to remove contaminants. 4. Forward and reverse DNA sequencing reaction of PCR amplicon was carried out with 16SF and 16SR primers using BDT v3.1 Cycle sequencing kit on ABI 3730xl Genetic Analyzer. 5. The 16S rDNA gene sequence was used to carry out BLAST with the nrdatabase of NCBI genbank database. Based on maximum identity score first ten sequences were selected. Data and Result: Sample was found to be Klebsiella pneumoniae strain based on nucleotide homology.
- 6. DATA: (Alignment view using combination of NCBI GenBank) Distribution of Blast Hits on the Query Sequence
- 7. Sequences producing significant alignments: Description Max Score Total Score Query Cover E value Per. Ident Accession Klebsiella pneumoniae strain STD 16S ribosomal RNA gene, partial sequence 827 827 55% 0 94.86% gi|1035548121|KX180916.1 Uncultured bacterium clone 16S-002-01-3A 16S ribosomal RNA gene, partial sequence 787 787 61% 0 90.43% gi|527180091|DQ866076.2 Klebsiella pneumoniae strain ACMJag2 16S ribosomal RNA gene, partial sequence 786 786 54% 0 93.66% gi|1735037174|MN410788. 1 Klebsiella pneumoniae strain TZT-18-59 16S ribosomal RNA gene, partial sequence 781 781 53% 0 93.47% gi|1477471365|MH922846.1 Bacterium strain BS1878 16S ribosomal RNA gene, partial sequence 780 780 54% 0 93.32% gi|1622984868|MK825066.1 Klebsiella pneumoniae strain CIFRI-ONBKP1 16S ribosomal RNA gene, partial sequence 779 779 53% 0 93.44% gi|1746155100|MN481047. 1 Bacterium strain BS1717 16S ribosomal RNA gene, partial sequence 779 779 53% 0 93.46% gi|1622984707|MK824905.1 Bacterium strain BS1712 16S ribosomal RNA gene, partial sequence 779 779 53% 0 93.46% gi|1622984702|MK824900.1 Klebsiella sp. CCFM8376 16S ribosomal RNA gene, partial sequence 779 779 53% 0 93.46% gi|660083258|KJ803933.1 Klebsiella pneumoniae strain CCFM8368 16S ribosomal RNA gene, partial sequence 779 779 53% 0 93.46% gi|660082048|KJ803925.1
- 10. File: BACTERIA-1_16S-1492R.ab1 Lane: 4 Base spacing: 13.40 Run Ended: Oct 16, 2019, 8:00:27 994 bases in 15538 scans Signal G:159 A:99 T:90 C:107 Page 1 of 3 Sample: BACTERIA-1_16S-1492R 10 20 30 40 50 60 70 80 90 100 GAA CC A AG TG G T A G C G C CC T CCC G AAG GTT AA G CTAC C T A C T T CT T T T G CAA CC CA C T C CCA TG G TG T G ACG G G CG G TG T GTACAA G G C CCG G G AACGTAT T CAC C 110 120 130 140 150 160 170 180 190 200 210 220 CG T AG CATT CT G AT CTACG ATTACTA G CG ATTCC GACTT CAT GG AGTCGAGTT GCAGACTCCAAT CCGG ACTACGACATACTTTAT GAG GTCCGCTTGCTCTCGCGAGGTCGCTTCTCTTT 230 240 250 260 270 280 290 300 310 320 330 340 350 TGTATAT GCCATTGTAGCACGTGTGTAGCCCTGGTCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCAGTTTATCACTGG CAGTCTCCTTT GAGTTCCCGGCCGAACCGCTGGCAACAAAGGA 360 370 380 390 400 410 420 430 440 450 460 470 480 ATAA GGGTTGCGCTCGTTGCGGGACTTAAC CAACA T T CACA A ACGAGCT GACGACAG C ATGCAC A C TGTCTCACAGTTC C G A GGCACC AT C ATCTCT GAAAGTTCTGT G A TGTC A GA CA GG AA G 490 500 510 520 530 540 550 560 570 580 GT C TCGCG T GC TCGAAT AAAC AC TGCC CACC GC T G GC G G CCC C TC A TTCAT T GAGT TT A CC T G G G C G ACT CCCA G CGG C G TTT A C G GT T GT C G GA GC ACG C TC AG GCAC 590 600 610 620 630 640 650 660 670 680 C ACCTC CAA CG ACT C T TAC G G T G AC TAC C G G A T T A AT CTG T TG T C C CAC CT T C AC TG A C G C G C T G C C G G G C C C T CT CCAC GG A T C TC C AA T C T C C T T C C
- 11. File: BACTERIA-1_16S-1492R.ab1 Lane: 4 Base spacing: 13.40 Run Ended: Oct 16, 2019, 8:00:27 994 bases in 15538 scans Signal G:159 A:99 T:90 C:107 Page 2 of 3 Sample: BACTERIA-1_16S-1492R 690 700 710 720 730 740 750 760 770 C G T C AC G GAT T T CC CC TC T AAA A T T A C TG CA T T A TG A T CC G GT G A CC G G A T C A T C A T T A AA C C T G G C T T C CA A AT C AT A C TT G C 770 780 790 800 810 820 830 840 C C C G TA C G C T T G C GA T A C G G C T T T G G A A A A A C A A GA T A A C T A C C TC C CC G A A G T T A A C G AG C T T C A C C C G A G T C A 850 860 870 880 890 900 910 G GT G C C A T G A TA C C T G G C C G GA G T G AG C G T A A C A G G G T C CT C A C A GA GA C G C A G A G A T T C C T A TA A T C T G C AT 920 930 940 950 960 970 T A G A G AC A C T C T T G C T A G T A T A G CT G A G T C T T A G T T C A AT T C C C C C C G A T C C T A C C A T A G T 980 990 G G C C G T C G G G C A T T A T A
- 12. File: BACTERIA-1_16S-1492R.ab1 Lane: 4 Base spacing: 13.40 Run Ended: Oct 16, 2019, 8:00:27 994 bases in 15538 scans Signal G:159 A:99 T:90 C:107 Page 3 of 3 Sample: BACTERIA-1_16S-1492R
- 13. BLASTN 2.10.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. RID: UKFVP3MM014 Database: Nucleotide collection (nt) 56,496,701 sequences; 253,942,268,922 total letters Query= BACTERIA-1_16S-1492R Length=994 Score E Max Sequences producing significant alignments: (Bits) Value Ident gi|1035548121|gb|KX180916.1| Klebsiella pneumoniae strain STD... 827 0.0 95% gi|1735037174|gb|MN410788.1| Klebsiella pneumoniae strain ACM... 786 0.0 94% gi|1477471365|gb|MH922846.1| Klebsiella pneumoniae strain TZT... 781 0.0 93% gi|1746155100|gb|MN481047.1| Klebsiella pneumoniae strain CIF... 779 0.0 93% gi|660083258|gb|KJ803933.1| Klebsiella sp. CCFM8376 16S ribos... 779 0.0 93% gi|660082048|gb|KJ803925.1| Klebsiella pneumoniae strain CCFM... 779 0.0 93% gi|458592281|gb|KC455426.1| Klebsiella sp. SFR-117 16S riboso... 779 0.0 93% gi|221579408|gb|FJ615552.1| Klebsiella sp. enrichment culture... 779 0.0 93% gi|1026746448|gb|KU253719.1| Klebsiella sp. OS1 16S ribosomal... 778 0.0 93% gi|458592291|gb|KC455429.1| Klebsiella sp. SR-142 16S ribosom... 778 0.0 93% ALIGNMENTS >gi|1035548121|gb|KX180916.1| Klebsiella pneumoniae strain STD 16S ribosomal RNA gene, partial sequence Length=1341 Score = 827 bits (916), Expect = 0.0
- 14. Identities = 535/564 (95%), Gaps = 18/564 (3%) Strand=Plus/Minus Query 7 AGTGGTAGCGCCCTCCCGAAGGTTAAGCTACCTACTTCTTTTGCAACCCACTCCCATGGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGTAGCATT CTGATCTACGATTACTAGCGATTCCGACTTCATGGAGTCGAG 156 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| |||||||||||||||||||||||||||||||||||||||||| Sbjct 1333 AGTGGTAGCGCCCTCCCGAAGGTTAAGCTACCTACTTCTTTTGCAACCCACTCCCATGGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGTAGCATT CTGATCTACGATTACTAGCGATTCCGACTTCATGGAGTCGAG 1184 Query 157 TTGCAGACTCCAATCCGGACTACGACATACTTTATGAGGTCCGCTTGCTCTCGCGAGGTCGCTTCTCTTTGTATATGCCATTGTAGCACGTGTGTAGCCCTGGTCGTA AGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCAGTT 306 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| |||||||||||||||||||||||||||||||||||||||||| Sbjct 1183 TTGCAGACTCCAATCCGGACTACGACATACTTTATGAGGTCCGCTTGCTCTCGCGAGGTCGCTTCTCTTTGTATATGCCATTGTAGCACGTGTGTAGCCCTGGTCGTA AGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCAGTT 1034 Query 307 TATCACTGGCAGTCTCCTTTGAGTTCCCGGCCGAACCGCTGGCAACAAAGGATAAGGGTTGCGCTCGTTGCGGGACTTAA-CCAACA-TTCACAA-ACGAGCTGACGA CAG-CATGCA-CA-CTGTCTCACAGTTCCG-AGGCACCA--T 447 |||||||||||||||||||||||||||||||| |||||||||||||||||||||||||||||||||||||||||||||| |||||| ||||||| ||||||||||||||| |||||| || |||||||||||||||| |||||||| | Sbjct 1033 TATCACTGGCAGTCTCCTTTGAGTTCCCGGCCTGACCGCTGGCAACAAAGGATAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATTTCACAACACGAGCTGACGA CAGCCATGCAGCACCTGTCTCACAGTTCCGAAGGCACCAATT 884 Query 448 CATCTCTGAAAGTTCTGTGATGTCAGA-CAGG-AAGTCTCGCGTGC-TCGAATAAACACTGC-CCACCGCTG-GCGGCCCCTCATTCATTGAGTTT-ACCTG-GGCG- ACTCCC 553 ||| | || ||| |||||||||||||| |||| ||||||||||||| |||| || |||||| ||||||||| ||||||| ||| ||||||||||| || || |||| |||||| Sbjct 883
- 15. CATTTTTGTAAG-TCTGTGATGTCAGACCAGGTAAGTCTCGCGTGCATCGATAAACCACTGCTCCACCGCTGTGCGGCCCGTCAATCATTGAGTTTAACATGCGGCGT ACTCCC 771 >gi|1735037174|gb|MN410788.1| Klebsiella pneumoniae strain ACMJag2 16S ribosomal RNA gene, partial sequence Length=1447 Score = 786 bits (871), Expect = 0.0 Identities = 532/568 (94%), Gaps = 30/568 (5%) Strand=Plus/Minus Query 1 GAACCAAGTGGTA-GCGCCCTCCCGAAGGTTAAGCTACCTACTTCTTTTGCAACCCACTCCCATGGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCG TAGCATTCTGATCTACGATTACTAGCGATTCCGACTTCATGG 149 || |||||||||| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| |||||||||||||||||||||||||||| Sbjct 1440 GATCCAAGTGGTAAGCGCCCTCCCGAAGGTTAAGCTACCTACTTCTTTTGCAACCCACTCCCATGGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCG TAGCATTCTGATCTACGATTACTAGCGATTCCGACTTCATGG 1291 Query 150 AGTCGAGTTGCAGACTCCAATCCGGACTACGACATACTTTATGAGGTCCGCTTGCTCTCGCGAGGTCGCTTCTCTTTGTATATGCCATTGTAGCACGTGTGTAGCCCT GGTCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCC 299 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| |||||||||||||||||||||||||||||||||||||||||| Sbjct 1290 AGTCGAGTTGCAGACTCCAATCCGGACTACGACATACTTTATGAGGTCCGCTTGCTCTCGCGAGGTCGCTTCTCTTTGTATATGCCATTGTAGCACGTGTGTAGCCCT GGTCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCC 1141 Query 300 TCCAGTTTATCACTGGCAGTCTCCTTTGAGTTCCCGGCCGAACCGCTGGCAACAAAGGATAAGGGTTGCGCTCGTTGCGGGACTTAA-CCAACA-TTCACAA-ACGAG CTGACGACAG-CATGCA-CA-CTGTCTCACAGTTCC--GAGG 441 ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| |||||| ||||||| ||||||||||||||| |||||| || ||||||||||||||| ||| Sbjct 1140
- 16. TCCAGTTTATCACTGGCAGTCTCCTTTGAGTTCCCGGCCGAACCGCTGGCAACAAAGGATAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATTTCACAACACGAG CTGACGACAGCCATGCAGCACCTGTCTCACAGTTCCCGAAGG 991 Query 442 CACCA--TCATCTCT-GAAAGTTCTGT-GATGTC-AGA-CAGG-AAG--TCTCGCG-TGC-TCGAATAAA-CAC-TGC-CCACCGCT--GGCGGCCCCTC----ATTC A-TTGAGTTT 538 ||||| ||||||| ||||||||||| |||||| ||| |||| ||| ||||||| ||| ||||||||| ||| ||| |||||||| |||| ||| | ||||| |||||||| Sbjct 990 CACCAATCCATCTCTGGAAAGTTCTGTGGATGTCAAGACCAGGTAAGGTTCTCGCGTTGCATCGAATAAACCACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTC ATTTGAGTTT 873 >gi|1477471365|gb|MH922846.1| Klebsiella pneumoniae strain TZT-18-59 16S ribosomal RNA gene, partial sequence Length=1439 Score = 781 bits (865), Expect = 0.0 Identities = 530/567 (93%), Gaps = 31/567 (5%) Strand=Plus/Minus Query 3 ACCAAGTGGTAGCGCCCTCCCGAAGGTTAAGCTACCTACTTCTTTTGCAACCCACTCCCATGGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGTAG CATTCTGATCTACGATTACTAGCGATTCCGACTTCATGGAGT 152 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| |||||||||||||||||||||||||||||||||||||||||| Sbjct 1432 ACCAAGTGGTAGCGCCCTCCCGAAGGTTAAGCTACCTACTTCTTTTGCAACCCACTCCCATGGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGTAG CATTCTGATCTACGATTACTAGCGATTCCGACTTCATGGAGT 1283 Query 153 CGAGTTGCAGACTCCAATCCGGACTACGACATACTTTATGAGGTCCGCTTGCTCTCGCGAGGTCGCTTCTCTTTGTATATGCCATTGTAGCACGTGTGTAGCCCTGGT CGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCC 302 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| |||||||||||||||||||||||||||||||||||||||||| Sbjct 1282
- 17. CGAGTTGCAGACTCCAATCCGGACTACGACATACTTTATGAGGTCCGCTTGCTCTCGCGAGGTCGCTTCTCTTTGTATATGCCATTGTAGCACGTGTGTAGCCCTGGT CGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCC 1133 Query 303 AGTTTATCACTGGCAGTCTCCTTTGAGTTCCCGGCCGAACCGCTGGCAACAAAGGATAAGGGTTGCGCTCGTTGCGGGACTTAA-CCAACA-TTCACAA-ACGAGCTG ACGACAG-CATGCA-CA-CTGTCTCACAGTTCC--GAGGCAC 444 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| |||||| ||||||| ||||||||||||||| |||||| || ||||||||||||||| |||||| Sbjct 1132 AGTTTATCACTGGCAGTCTCCTTTGAGTTCCCGGCCGAACCGCTGGCAACAAAGGATAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATTTCACAACACGAGCTG ACGACAGCCATGCAGCACCTGTCTCACAGTTCCCGAAGGCAC 983 Query 445 CA--TCATCTCT-GAAAGTTCTGT-GATGTC-AGA-CAGG-AAG--TC-TCGCG-TGC-TCGAAT--AAACAC-TGC-CCACCGCT--GGCGGCCCCTC----ATTCA -TTGAGTTT 538 || ||||||| ||||||||||| |||||| ||| |||| ||| || ||||| ||| |||||| || ||| ||| |||||||| |||| ||| | ||||| |||||||| Sbjct 982 CAATCCATCTCTGGAAAGTTCTGTGGATGTCAAGACCAGGTAAGGTTCTTCGCGTTGCATCGAATTAAACCACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCA TTTGAGTTT 866 >gi|1746155100|gb|MN481047.1| Klebsiella pneumoniae strain CIFRI-ONBKP1 16S ribosomal RNA gene, partial sequence Length=1411 Score = 779 bits (863), Expect = 0.0 Identities = 527/564 (93%), Gaps = 30/564 (5%) Strand=Plus/Minus Query 5 CAAGTGGTAGCGCCCTCCCGAAGGTTAAGCTACCTACTTCTTTTGCAACCCACTCCCATGGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGTAGCA TTCTGATCTACGATTACTAGCGATTCCGACTTCATGGAGTCG 154 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| |||||||||||||||||||||||||||||||||||||||||| Sbjct 1409 CAAGTGGTAGCGCCCTCCCGAAGGTTAAGCTACCTACTTCTTTTGCAACCCACTCCCATGGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGTAGCA
- 18. TTCTGATCTACGATTACTAGCGATTCCGACTTCATGGAGTCG 1260 Query 155 AGTTGCAGACTCCAATCCGGACTACGACATACTTTATGAGGTCCGCTTGCTCTCGCGAGGTCGCTTCTCTTTGTATATGCCATTGTAGCACGTGTGTAGCCCTGGTCG TAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCAG 304 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| |||||||||||||||||||||||||||||||||||||||||| Sbjct 1259 AGTTGCAGACTCCAATCCGGACTACGACATACTTTATGAGGTCCGCTTGCTCTCGCGAGGTCGCTTCTCTTTGTATATGCCATTGTAGCACGTGTGTAGCCCTGGTCG TAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCAG 1110 Query 305 TTTATCACTGGCAGTCTCCTTTGAGTTCCCGGCCGAACCGCTGGCAACAAAGGATAAGGGTTGCGCTCGTTGCGGGACTTAA-CCAACA-TTCACAA-ACGAGCTGAC GACAG-CATGCA-CA-CTGTCTCACAGTTCC--GAGGCACCA 446 |||||||||||||||||||||||||||||||||| ||||||||||||||||||||||||||||||||||||||||||||||| |||||| ||||||| ||||||||||||||| |||||| || ||||||||||||||| |||||||| Sbjct 1109 TTTATCACTGGCAGTCTCCTTTGAGTTCCCGGCCTAACCGCTGGCAACAAAGGATAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATTTCACAACACGAGCTGAC GACAGCCATGCAGCACCTGTCTCACAGTTCCCGAAGGCACCA 960 Query 447 --TCATCTCT-GAAAGTTCTGT-GATGTC-AGA-CAGG-AAG--TC-TCGCG-TGC-TCGAAT--AAACAC-TGC-CCACCGCT--GGCGGCCCCTC----ATTCATT GAGTTT 538 ||||||| ||||||||||| |||||| ||| |||| ||| || ||||| ||| |||||| || ||| ||| |||||||| |||| ||| | ||||||||||||| Sbjct 959 ATCCATCTCTGGAAAGTTCTGTGGATGTCAAGACCAGGTAAGGTTCTTCGCGTTGCATCGAATTAAACCACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCATT GAGTTT 846 >gi|660083258|gb|KJ803933.1| Klebsiella sp. CCFM8376 16S ribosomal RNA gene, partial sequence Length=1422 Score = 779 bits (863), Expect = 0.0 Identities = 529/566 (93%), Gaps = 31/566 (5%) Strand=Plus/Minus
- 19. Query 4 CCAAGTGGTAGCGCCCTCCCGAAGGTTAAGCTACCTACTTCTTTTGCAACCCACTCCCATGGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGTAGC ATTCTGATCTACGATTACTAGCGATTCCGACTTCATGGAGTC 153 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| |||||||||||||||||||||||||||||||||||||||||| Sbjct 1422 CCAAGTGGTAGCGCCCTCCCGAAGGTTAAGCTACCTACTTCTTTTGCAACCCACTCCCATGGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGTAGC ATTCTGATCTACGATTACTAGCGATTCCGACTTCATGGAGTC 1273 Query 154 GAGTTGCAGACTCCAATCCGGACTACGACATACTTTATGAGGTCCGCTTGCTCTCGCGAGGTCGCTTCTCTTTGTATATGCCATTGTAGCACGTGTGTAGCCCTGGTC GTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCA 303 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| |||||||||||||||||||||||||||||||||||||||||| Sbjct 1272 GAGTTGCAGACTCCAATCCGGACTACGACATACTTTATGAGGTCCGCTTGCTCTCGCGAGGTCGCTTCTCTTTGTATATGCCATTGTAGCACGTGTGTAGCCCTGGTC GTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCA 1123 Query 304 GTTTATCACTGGCAGTCTCCTTTGAGTTCCCGGCCGAACCGCTGGCAACAAAGGATAAGGGTTGCGCTCGTTGCGGGACTTAA-CCAACA-TTCACAA-ACGAGCTGA CGACAG-CATGCA-CA-CTGTCTCACAGTTCC--GAGGCACC 445 ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| |||||| ||||||| ||||||||||||||| |||||| || ||||||||||||||| ||||||| Sbjct 1122 GTTTATCACTGGCAGTCTCCTTTGAGTTCCCGGCCGAACCGCTGGCAACAAAGGATAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATTTCACAACACGAGCTGA CGACAGCCATGCAGCACCTGTCTCACAGTTCCCGAAGGCACC 973 Query 446 A--TCATCTCT-GAAAGTTCTGT-GATGTC-AGA-CAGG-AAG--TC-TCGCG-TGC-TCGAAT--AAACAC-TGC-CCACCGCT--GGCGGCCCCTC----ATTCA- TTGAGTTT 538 | ||||||| ||||||||||| |||||| ||| |||| ||| || ||||| ||| |||||| || ||| ||| |||||||| |||| ||| | ||||| |||||||| Sbjct 972 AATCCATCTCTGGAAAGTTCTGTGGATGTCAAGACCAGGTAAGGTTCTTCGCGTTGCATCGAATTAAACCACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCAT TTGAGTTT 857
- 20. >gi|660082048|gb|KJ803925.1| Klebsiella pneumoniae strain CCFM8368 16S ribosomal RNA gene, partial sequence Length=1426 Score = 779 bits (863), Expect = 0.0 Identities = 529/566 (93%), Gaps = 31/566 (5%) Strand=Plus/Minus Query 4 CCAAGTGGTAGCGCCCTCCCGAAGGTTAAGCTACCTACTTCTTTTGCAACCCACTCCCATGGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGTAGC ATTCTGATCTACGATTACTAGCGATTCCGACTTCATGGAGTC 153 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| |||||||||||||||||||||||||||||||||||||||||| Sbjct 1424 CCAAGTGGTAGCGCCCTCCCGAAGGTTAAGCTACCTACTTCTTTTGCAACCCACTCCCATGGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGTAGC ATTCTGATCTACGATTACTAGCGATTCCGACTTCATGGAGTC 1275 Query 154 GAGTTGCAGACTCCAATCCGGACTACGACATACTTTATGAGGTCCGCTTGCTCTCGCGAGGTCGCTTCTCTTTGTATATGCCATTGTAGCACGTGTGTAGCCCTGGTC GTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCA 303 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| |||||||||||||||||||||||||||||||||||||||||| Sbjct 1274 GAGTTGCAGACTCCAATCCGGACTACGACATACTTTATGAGGTCCGCTTGCTCTCGCGAGGTCGCTTCTCTTTGTATATGCCATTGTAGCACGTGTGTAGCCCTGGTC GTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCA 1125 Query 304 GTTTATCACTGGCAGTCTCCTTTGAGTTCCCGGCCGAACCGCTGGCAACAAAGGATAAGGGTTGCGCTCGTTGCGGGACTTAA-CCAACA-TTCACAA-ACGAGCTGA CGACAG-CATGCA-CA-CTGTCTCACAGTTCC--GAGGCACC 445 ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| |||||| ||||||| ||||||||||||||| |||||| || ||||||||||||||| ||||||| Sbjct 1124 GTTTATCACTGGCAGTCTCCTTTGAGTTCCCGGCCGAACCGCTGGCAACAAAGGATAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATTTCACAACACGAGCTGA CGACAGCCATGCAGCACCTGTCTCACAGTTCCCGAAGGCACC 975
- 21. Query 446 A--TCATCTCT-GAAAGTTCTGT-GATGTC-AGA-CAGG-AAG--TC-TCGCG-TGC-TCGAAT--AAACAC-TGC-CCACCGCT--GGCGGCCCCTC----ATTCA- TTGAGTTT 538 | ||||||| ||||||||||| |||||| ||| |||| ||| || ||||| ||| |||||| || ||| ||| |||||||| |||| ||| | ||||| |||||||| Sbjct 974 AATCCATCTCTGGAAAGTTCTGTGGATGTCAAGACCAGGTAAGGTTCTTCGCGTTGCATCGAATTAAACCACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCAT TTGAGTTT 859 >gi|458592281|gb|KC455426.1| Klebsiella sp. SFR-117 16S ribosomal RNA gene, partial sequence Length=1436 Score = 779 bits (863), Expect = 0.0 Identities = 529/566 (93%), Gaps = 31/566 (5%) Strand=Plus/Minus Query 4 CCAAGTGGTAGCGCCCTCCCGAAGGTTAAGCTACCTACTTCTTTTGCAACCCACTCCCATGGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGTAGC ATTCTGATCTACGATTACTAGCGATTCCGACTTCATGGAGTC 153 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| |||||||||||||||||||||||||||||||||||||||||| Sbjct 1430 CCAAGTGGTAGCGCCCTCCCGAAGGTTAAGCTACCTACTTCTTTTGCAACCCACTCCCATGGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGTAGC ATTCTGATCTACGATTACTAGCGATTCCGACTTCATGGAGTC 1281 Query 154 GAGTTGCAGACTCCAATCCGGACTACGACATACTTTATGAGGTCCGCTTGCTCTCGCGAGGTCGCTTCTCTTTGTATATGCCATTGTAGCACGTGTGTAGCCCTGGTC GTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCA 303 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| |||||||||||||||||||||||||||||||||||||||||| Sbjct 1280 GAGTTGCAGACTCCAATCCGGACTACGACATACTTTATGAGGTCCGCTTGCTCTCGCGAGGTCGCTTCTCTTTGTATATGCCATTGTAGCACGTGTGTAGCCCTGGTC GTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCA 1131 Query 304
- 22. GTTTATCACTGGCAGTCTCCTTTGAGTTCCCGGCCGAACCGCTGGCAACAAAGGATAAGGGTTGCGCTCGTTGCGGGACTTAA-CCAACA-TTCACAA-ACGAGCTGA CGACAG-CATGCA-CA-CTGTCTCACAGTTCC--GAGGCACC 445 ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| |||||| ||||||| ||||||||||||||| |||||| || ||||||||||||||| ||||||| Sbjct 1130 GTTTATCACTGGCAGTCTCCTTTGAGTTCCCGGCCGAACCGCTGGCAACAAAGGATAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATTTCACAACACGAGCTGA CGACAGCCATGCAGCACCTGTCTCACAGTTCCCGAAGGCACC 981 Query 446 A--TCATCTCT-GAAAGTTCTGT-GATGTC-AGA-CAGG-AAG--TC-TCGCG-TGC-TCGAAT--AAACAC-TGC-CCACCGCT--GGCGGCCCCTC----ATTCA- TTGAGTTT 538 | ||||||| ||||||||||| |||||| ||| |||| ||| || ||||| ||| |||||| || ||| ||| |||||||| |||| ||| | ||||| |||||||| Sbjct 980 AATCCATCTCTGGAAAGTTCTGTGGATGTCAAGACCAGGTAAGGTTCTTCGCGTTGCATCGAATTAAACCACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCAT TTGAGTTT 865 >gi|221579408|gb|FJ615552.1| Klebsiella sp. enrichment culture clone Xuan01 16S ribosomal RNA gene, partial sequence Length=1434 Score = 779 bits (863), Expect = 0.0 Identities = 529/566 (93%), Gaps = 31/566 (5%) Strand=Plus/Minus Query 4 CCAAGTGGTAGCGCCCTCCCGAAGGTTAAGCTACCTACTTCTTTTGCAACCCACTCCCATGGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGTAGC ATTCTGATCTACGATTACTAGCGATTCCGACTTCATGGAGTC 153 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| |||||||||||||||||||||||||||||||||||||||||| Sbjct 1432 CCAAGTGGTAGCGCCCTCCCGAAGGTTAAGCTACCTACTTCTTTTGCAACCCACTCCCATGGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGTAGC ATTCTGATCTACGATTACTAGCGATTCCGACTTCATGGAGTC 1283 Query 154 GAGTTGCAGACTCCAATCCGGACTACGACATACTTTATGAGGTCCGCTTGCTCTCGCGAGGTCGCTTCTCTTTGTATATGCCATTGTAGCACGTGTGTAGCCCTGGTC
- 23. GTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCA 303 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| |||||||||||||||||||||||||||||||||||||||||| Sbjct 1282 GAGTTGCAGACTCCAATCCGGACTACGACATACTTTATGAGGTCCGCTTGCTCTCGCGAGGTCGCTTCTCTTTGTATATGCCATTGTAGCACGTGTGTAGCCCTGGTC GTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCA 1133 Query 304 GTTTATCACTGGCAGTCTCCTTTGAGTTCCCGGCCGAACCGCTGGCAACAAAGGATAAGGGTTGCGCTCGTTGCGGGACTTAA-CCAACA-TTCACAA-ACGAGCTGA CGACAG-CATGCA-CA-CTGTCTCACAGTTCC--GAGGCACC 445 ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| |||||| ||||||| ||||||||||||||| |||||| || ||||||||||||||| ||||||| Sbjct 1132 GTTTATCACTGGCAGTCTCCTTTGAGTTCCCGGCCGAACCGCTGGCAACAAAGGATAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATTTCACAACACGAGCTGA CGACAGCCATGCAGCACCTGTCTCACAGTTCCCGAAGGCACC 983 Query 446 --ATCATCTCT-GAAAGTTCTGT-GATGTC-AGA-CAGG-AAG--TC-TCGCG-TGC-TCGAAT--AAACAC-TGC-CCACCGCT--GGCGGCCCCTC----ATTCA- TTGAGTTT 538 | ||||||| ||||||||||| |||||| ||| |||| ||| || ||||| ||| |||||| || ||| ||| |||||||| |||| ||| | ||||| |||||||| Sbjct 982 AAACCATCTCTGGAAAGTTCTGTGGATGTCAAGACCAGGTAAGGTTCTTCGCGTTGCATCGAATTAAACCACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCAT TTGAGTTT 867 >gi|1026746448|gb|KU253719.1| Klebsiella sp. OS1 16S ribosomal RNA gene, partial sequence Length=1431 Score = 778 bits (862), Expect = 0.0 Identities = 530/568 (93%), Gaps = 31/568 (5%) Strand=Plus/Minus Query 2 AACCAAGTGGTAGCGCCCTCCCGAAGGTTAAGCTACCTACTTCTTTTGCAACCCACTCCCATGGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGTA GCATTCTGATCTACGATTACTAGCGATTCCGACTTCATGGAG 151 ||
- 24. |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| ||||||||||||||||||||||||||||||||||||||| Sbjct 1431 AATCAAGTGGTAGCGCCCTCCCGAAGGTTAAGCTACCTACTTCTTTTGCAACCCACTCCCATGGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGTA GCATTCTGATCTACGATTACTAGCGATTCCGACTTCATGGAG 1282 Query 152 TCGAGTTGCAGACTCCAATCCGGACTACGACATACTTTATGAGGTCCGCTTGCTCTCGCGAGGTCGCTTCTCTTTGTATATGCCATTGTAGCACGTGTGTAGCCCTGG TCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTC 301 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| |||||||||||||||||||||||||||||||||||||||||| Sbjct 1281 TCGAGTTGCAGACTCCAATCCGGACTACGACATACTTTATGAGGTCCGCTTGCTCTCGCGAGGTCGCTTCTCTTTGTATATGCCATTGTAGCACGTGTGTAGCCCTGG TCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTC 1132 Query 302 CAGTTTATCACTGGCAGTCTCCTTTGAGTTCCCGGCCGAACCGCTGGCAACAAAGGATAAGGGTTGCGCTCGTTGCGGGACTTAA-CCAACA-TTCACAA-ACGAGCT GACGACAG-CATGCA-CA-CTGTCTCACAGTTCC--GAGGCA 443 ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| |||||| ||||||| ||||||||||||||| |||||| || ||||||||||||||| ||||| Sbjct 1131 CAGTTTATCACTGGCAGTCTCCTTTGAGTTCCCGGCCGAACCGCTGGCAACAAAGGATAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATTTCACAACACGAGCT GACGACAGCCATGCAGCACCTGTCTCACAGTTCCCGAAGGCA 982 Query 444 CCA--TCATCTCT-GAAAGTTCTGT-GATGTC-AGA-CAGG-AAG--TC-TCGCG-TGC-TCGAAT--AAACAC-TGC-CCACCGCT--GGCGGCCCCTC----ATTC A-TTGAGTTT 538 ||| ||||||| ||||||||||| |||||| ||| |||| ||| || ||||| ||| |||||| || ||| ||| |||||||| |||| ||| | ||||| |||||||| Sbjct 981 CCAATCCATCTCTGGAAAGTTCTGTGGATGTCAAGACCAGGTAAGGTTCTTCGCGTTGCATCGAATTAAACCACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTC ATTTGAGTTT 864 >gi|458592291|gb|KC455429.1| Klebsiella sp. SR-142 16S ribosomal RNA gene, partial sequence Length=1439
- 25. Score = 778 bits (862), Expect = 0.0 Identities = 530/568 (93%), Gaps = 31/568 (5%) Strand=Plus/Minus Query 2 AACCAAGTGGTAGCGCCCTCCCGAAGGTTAAGCTACCTACTTCTTTTGCAACCCACTCCCATGGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGTA GCATTCTGATCTACGATTACTAGCGATTCCGACTTCATGGAG 151 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| |||||||||||||||||||||||||||||||||||||||||| Sbjct 1434 AACCAAGTGGTAGCGCCCTCCCGAAGGTTAAGCTACCTACTTCTTTTGCAACCCACTCCCATGGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGTA GCATTCTGATCTACGATTACTAGCGATTCCGACTTCATGGAG 1285 Query 152 TCGAGTTGCAGACTCCAATCCGGACTACGACATACTTTATGAGGTCCGCTTGCTCTCGCGAGGTCGCTTCTCTTTGTATATGCCATTGTAGCACGTGTGTAGCCCTGG TCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTC 301 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| |||||||||||||||||||||||||||||||||||||||||| Sbjct 1284 TCGAGTTGCAGACTCCAATCCGGACTACGACATACTTTATGAGGTCCGCTTGCTCTCGCGAGGTCGCTTCTCTTTGTATATGCCATTGTAGCACGTGTGTAGCCCTGG TCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTC 1135 Query 302 CAGTTTATCACTGGCAGTCTCCTTTGAGTTCCCGGCCGAACCGCTGGCAACAAAGGATAAGGGTTGCGCTCGTTGCGGGACTTAA-CCAACA-TTCACAA-ACGAGCT GACGACAG-CATGCA-CA-CTGTCTCACAGTTCC--GAGGCA 443 ||||||||||||||||||||||||||||||||||||| ||||||||||||||||||||||||||||||||||||||||||||||| |||||| ||||||| ||||||||||||||| |||||| || ||||||||||||||| ||||| Sbjct 1134 CAGTTTATCACTGGCAGTCTCCTTTGAGTTCCCGGCCTAACCGCTGGCAACAAAGGATAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATTTCACAACACGAGCT GACGACAGCCATGCAGCACCTGTCTCACAGTTCCCGAAGGCA 985 Query 444 CCA--TCATCTCT-GAAAGTTCTGT-GATGTC-AGA-CAGG-AAG--TC-TCGCG-TGC-TCGAAT--AAACAC-TGC-CCACCGCT--GGCGGCCCCTC----ATTC A-TTGAGTTT 538 ||| ||||||| ||||||||||| |||||| ||| |||| ||| || ||||| ||| |||||| || ||| ||| |||||||| |||| ||| | ||||| ||||||||
- 26. Sbjct 984 CCAATCCATCTCTGGAAAGTTCTGTGGATGTCAAGACCAGGTAAGGTTCTTCGCGTTGCATCGAATTAAACCACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTC ATTTGAGTTT 867 Database: Nucleotide collection (nt) Posted date: Oct 3, 2019 5:47 AM Number of letters in database: 253,942,268,922 Number of sequences in database: 56,496,701 Lambda K H 0.634 0.408 0.912 Gapped Lambda K H 0.625 0.410 0.780 Matrix: blastn matrix:2 -3 Gap Penalties: Existence: 5, Extension: 2 Number of Sequences: 56496701 Number of Hits to DB: 24333722 Number of extensions: 55084 Number of successful extensions: 55084 Number of sequences better than 10: 5582 Number of HSP's better than 10 without gapping: 0 Number of HSP's gapped: 40446 Number of HSP's successfully gapped: 7148 Length of query: 994 Length of database: 253942268922 Length adjustment: 39 Effective length of query: 955 Effective length of database: 251738897583 Effective search space: 240410647191765 Effective search space used: 240410647191765 A: 0 X1: 22 (20.1 bits) X2: 33 (29.8 bits) X3: 110 (99.2 bits) S1: 28 (26.5 bits) S2: 48 (44.6 bits)