1. 1 2 3 4 5 6 7 8 9 10 11 12
HA positive
HA negative
Positive control
wells (A 12 to D12)
Negative control
wells (E 12 to H12)
A
B
C
D
E
F
G
H
Hemagglutination test

2. AIV positve sample AIV negative sample
Avian influenza rapid antigen detection test

3. M 1 2 3 4 5 6 7 8 9 10 11 12 13 14 M
Subtyping of AIV cloacal samples using -RT-PCR-degenerate primers
1550
HA (1778 bp)
NA (1413 bp)
1400
2000
1000
750
M (1027 bp)
NSP (890 bp)

4. Subtyping of AIV cloacal samples using -RT-PCR-degenerate primers
2000
1550
1400
1000
750
M 1 2 3 4 5 6 7 M

5. Subtyping of AIV cloacal samples using -RT-PCR-NA-Hoffmann primers
2000
1550
1400
1000
M 1 2 3 4 5 6 7 8 9 10 M
NA (1413 bp)

6. Subtyping of AIV cloacal samples using -RT-PCR-NA-Hoffmann primers
2000
1550
1000
1400
M 1 2 3 4 5 6 7 8 9 10
M
NA (1413 bp)

7. AIV Matrix-RT-PCR
1000
M 1 2 3 4 5 6 7 8 9 10 M
1400 M (1027 bp)

8. AIV Matrix-RT-PCR
1400
1000
M 1 2 3 4 5 6 7 8 9 10 M
M (1027 bp)

9. AIV Matrix--RT-PCR
M 1 2 3 4 5 6 7 8 9 10 11 12 13 14 M
1400
1000
M 1 2 3 4 5 6 7 8 9 10 11 12 M
M (1027 bp)

10. Subtyping of AIV cloacal samples using -RT-PCR-HA-Hoffmann primers
2000
1400
1000
M 1 2 3 4 5 6 7 8 9 10 11 12 13 14 M
HA (1778 bp)
M 1 2 3 4 5 6 7
1550

11. Cloacal samples AIV subtyping-RT-PCR-HA-Hoffmann primers
M 1 2 3 4 5 6 7 8 9 10 11 12 13 14 M
M 1 2 3 4 5 6 7
2000
1400
1000
1550
HA (1778 bp)

12. 2000
1400
1000
1550
HA (1778 bp)
Subtyping of AIV cloacal samples using -RT-PCR-HA-Hoffmann primers
M 1 2 3 4 5 6 7

13. 2000
1400
1000
1550
HA (1778 bp)
Subtyping of AIV cloacal samples using -RT-PCR-HA-Hoffmann primers
M 1 2 3 4 M

14. 500
1000
750
HA (640 bp)
Subtyping of AIV cloacal samples using RT-PCR-HA-Phipps primers
M 1 2 3 4 5 6 7
M

15. 500
1000
750
HA (640 bp)
Cloacal samples AIV subtyping-RT-PCR-HA-Phipps primers
M 1 2 3 4 5 6 7 M

16. AIV HA and NA DNA after purification of the PCR bands for sequencing
M 1 2 3 4 5 6 7 8 9 10 11 12 13 14 M
2000
1400
1000
1550
HA (1778 bp)
NA (1413 bp)
M 1 2 3 M 4 5 6 7 8 9 10 11 12 13 M

17. AIV-RT-PCR-H5-cleavege site
M 1 2 3 4
300
400
HA cleavage site (249 bp)
200

18. AIV-RT-PCR-H7-cleavege site
M 1 2 3 4 5 6 7 8 9 10 11 12 13 14 M
300
400
200
HA cleavage site (241bp)
M 1 2 3 4

19. AIV subtyping-RT-PCR-NA.2.5.Chander primers
500
M 1 2 3 4 5 6 7 8 9 10 11 12 M
NA (536 bp)

20. AIV subtyping-RT-PCR-NA.8.1.Chander primers
M 1 2 3 4 5 6 7 8 9 10 11 12 13 14 M
500
NA (429 bp)
M 1 2 3 4 5 6 7 8 9 10 11 12 M

21. AIV sub typing RT-PCR-NA 6.1.Chander primers
500
M 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 M
NA (442 bp)

22. NDV RT-PCR
400
300
M 1 2 3 4 5 6 7 8 9 10 11 12 13 14 M
NDV F gene (340 bp)
M 1 2 3 4 5 6 7 8 9 10 M

23. NDV RT-PCR before treatment of AIV-NDV co infected samples with NDV antiserum
NDV F gene (340 bp)
NDV RT-PCR after treatment of AIV-NDV co infected samples with NDV antiserum
M 1 2 3 4 5 6 7 8 9 10 11 12 M