FIELD EVALUATION OF INGELVAC CIRCOFLEX ® ON GROWTH PERFORMANCE OF PIGS IN A SUBCLINICAL PCVAD HERD John Waddell, DVM, MBA

Determine the impact of one-dose of Ingelvac CircoFLEX® on growth performance of pigs in a “subclinical” PCVAD population Determine if population weight distribution can be affected by immunization against PCV2 Objectives

Determine the impact of one-dose of Ingelvac CircoFLEX® on growth performance of pigs in a “subclinical” PCVAD population

Determine if population weight distribution can be affected by immunization against PCV2

Source farm is PRRSV naïve with routine monitoring 600 weaned pigs ( 21 days of age) were tagged, weighed and randomized at weaning by weight and sex 300 vaccinates / 300 controls Pigs were commingled within pens throughout the study Individual body weights were taken at day 0 , day 35 , and day 119 Cull pigs were defined as weighing less than 180 lbs on day 119 Material and Method

Source farm is PRRSV naïve with routine monitoring

600 weaned pigs ( 21 days of age) were tagged, weighed and randomized at weaning by weight and sex

300 vaccinates / 300 controls

Pigs were commingled within pens throughout the study

Individual body weights were taken at day 0 , day 35 , and day 119

Cull pigs were defined as weighing less than 180 lbs on day 119

Source farm is PRRSV naïve with routine monitoring 600 weaned pigs ( 21 days of age) were tagged, weighed and randomized at weaning by weight and sex 300 vaccinates / 300 controls Pigs were commingled within pens throughout the study Individual body weights were taken at day 0 , day 35 , and day 119 Cull pigs were defined as weighing less than 180 lbs on day 119 Material and Method

Source farm is PRRSV naïve with routine monitoring

600 weaned pigs ( 21 days of age) were tagged, weighed and randomized at weaning by weight and sex

300 vaccinates / 300 controls

Pigs were commingled within pens throughout the study

Individual body weights were taken at day 0 , day 35 , and day 119

Cull pigs were defined as weighing less than 180 lbs on day 119

Source farm is PRRSV naïve with routine monitoring 600 weaned pigs ( 21 days of age) were tagged, weighed and randomized at weaning by weight and sex 300 vaccinates / 300 controls Pigs were commingled within pens throughout the study Individual body weights were taken at day 0 , day 35 , and day 119 Cull pigs were defined as weighing less than 180 lbs on day 119 Material and Method

Source farm is PRRSV naïve with routine monitoring

600 weaned pigs ( 21 days of age) were tagged, weighed and randomized at weaning by weight and sex

300 vaccinates / 300 controls

Pigs were commingled within pens throughout the study

Individual body weights were taken at day 0 , day 35 , and day 119

Cull pigs were defined as weighing less than 180 lbs on day 119

Serology

Serology 93 randomly selected pigs Controlled for treatment group and sex Six serial blood collection throughout study PCV2, PRRS, Salmonella, Lawsonia and Mycoplasma

93 randomly selected pigs

Controlled for treatment group and sex

Six serial blood collection throughout study

PCV2, PRRS, Salmonella, Lawsonia and Mycoplasma

Results

1 Cull rate based on animals weighing less than 180 lbs on d119 Mortality and cull rate (Fisher's Exact Test) Results Variable Vaccinates Controls P-value Mortality Rate (%) 3.01 3.86 0.22 Cull Rate¹ (%) 1.34 6.69 0.001

1 Probability of a greater F-statistic Least square means for performance data ab Within a row, means without a common superscript letter differ (Student's t, P≤ 0.05) Results Variable Vaccinates Controls P-Value¹ Day 0 weight (lbs) 16.17 16.17 0.97 Day 35 weight (lbs) 50.83 50.79 0.94 Day 119 weight (lbs) 227.25 a 220.86 b 0.0005 Day 0-35 ADG (lbs) 0.99 0.99 0.91 Day 36-119 ADG (lbs) 2.10 a 2.02 b <0.0001 Day 0-119 ADG (lbs) 1.77 a 1.72 b 0.0006 Day 0-119 wt gain (lbs) 210.71 a 204.38 b 0.0006

Results Coefficient of Variation (CV) = 10.64(Vaccinated); 12.93 (Non-Vaccinated)

PCV2 Quantitative PCR a a a b b b Log Titer Weeks Post-Vaccination

The one dose PCV2 vaccine significantly increased growth rate (weight gain and ADG) in a population of high health pigs which do not meet the case definition of (clinical) PCVAD. Culling rate was significantly reduced by vaccination. Consistent with a diagnosis of subclinical PCVAD, the mortality rate did not significantly differ and this was not a primary complaint of the producer. Conclusions

The one dose PCV2 vaccine significantly increased growth rate (weight gain and ADG) in a population of high health pigs which do not meet the case definition of (clinical) PCVAD.

Culling rate was significantly reduced by vaccination.

Consistent with a diagnosis of subclinical PCVAD, the mortality rate did not significantly differ and this was not a primary complaint of the producer.

The right shift of weights and narrowing of weight range distribution for the vaccinated group of pigs illustrates the biologic and implied economic benefit of immunization. Conclusions

The right shift of weights and narrowing of weight range distribution for the vaccinated group of pigs illustrates the biologic and implied economic benefit of immunization.