2,dna replication

Chen Yonggang Zhejiang University Schools of Medicine Biochemistry

DNA Replication-Conservation of Information ,[object Object],[object Object],[object Object],[object Object],[object Object]

DNA is replicated in a semi-conservative manner ,[object Object],McKee 18.2

Each separated DNA strand is duplicated to give two new double helices.

DNA semiconservative replication ,[object Object]

The process which appeared simple initially is complex ,[object Object],[object Object],[object Object],McKee 17.16

To allow the replication to occur supercoiling must be relaxed ,[object Object],[object Object],[object Object]

The first step in replication involves oriC ,[object Object],[object Object],[object Object],[object Object]

dnaA allows binding of two other important proteins ,[object Object],[object Object],[object Object]

SSB, a single strand binding tetramer stabilizes the initiation complex McKee 18.7

DNA replication involves many enzymes ,[object Object],[object Object]

At the replication fork two strands are managed differently ,[object Object],[object Object],[object Object]

The replication fork McKee 18.6

DNA Polymerase III holoenzyme contains 10 distinct types of subunits ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

The Pol III synthesizes DNA from dNTPs McKee 18.3

The Dimer moves in one direction and synthesizes 5’-3’ ,[object Object],[object Object],[object Object]

DNA polymerase III forms phosphodiester bonds ,[object Object],[object Object],[object Object]

Synthesis of a phosphodiester bond

DNA Polymerase III is at the center of Replication McKee 18.8

While DNA polymerase III does the replicating, DNAP I cleans up ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

The three activities are on one polypeptide ,[object Object],[object Object]

As Pol III finishes, Pol I goes to work ,[object Object],[object Object],[object Object]

Supercoiling was taken out by dnaB, DNA gyrase replaces it ,[object Object],[object Object]

DNA ligase seals the Okazaki fragments and the completed double helical DNA ,[object Object],[object Object],[object Object],[object Object]

Eucaryotic Replication is similar to that of procaryotes ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Eucaryotic replication is distinct from that of procaryotes ,[object Object],[object Object],[object Object],[object Object],[object Object]

Initiation of replication occurs at multiple ori ,[object Object],[object Object],[object Object],[object Object],[object Object]

Binding of initiation factors to the lagging strand differs ,[object Object],[object Object],[object Object],[object Object],[object Object]

Finishing and sealing of the lagging strand is different ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Telomeres are GC rich self-complementary sequences at chromosome ends ,[object Object],[object Object],[object Object],[object Object],[object Object]

2,dna replication

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2,dna replication