Primer Designing

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mohammad shenagari

Primer Design

1. Gene Runner 2. Oligo 6 4. Manual 3. Primer 3(on line)

Gene Runner 1.Sequence Insertion

Oligo 6

Manual 1. Reference sequence & other sequences (NCBI) 2. Alignment (Mega 4) 3. Sequence selection (In selected gene) 4. Blast (NCBI) 5. TM ( oligo analyzer , oligo6, gene runner) 6. Secondary structures (loop, hairpin,…) 7. False priming (oligo 6)

HCV Genome

Alignment

Current oligo length Position number

Non specific band

- Length ,[object Object],[object Object]

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Primer Designing

1. Primer Design

2. 1. Gene Runner 2. Oligo 6 4. Manual 3. Primer 3(on line)

3. Gene Runner 1.Sequence Insertion

4.

5.

6.

7.

8.

9.

11.

12.

13.

14.

15.

16.

17. Manual 1. Reference sequence & other sequences (NCBI) 2. Alignment (Mega 4) 3. Sequence selection (In selected gene) 4. Blast (NCBI) 5. TM ( oligo analyzer , oligo6, gene runner) 6. Secondary structures (loop, hairpin,…) 7. False priming (oligo 6)

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21. Current oligo length Position number

22. Non specific band

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