Vishram Singh - Textbook of Anatomy Upper Limb and Thorax.. Volume 1 (1).pdf
Metal Ion Lab Interpretation Of Results
1. Interpretation of Results
Retention time (from graph)
Cu2+ = 2172 sec.
Fe2+ = 2351 sec.
Linear velocity of mobile phase
U = L/tm
L = length of resin bed
tm = first unretained species
U = 13.3cm/1564s
U = 8.504 x 10-3cms-1
Average Linear rate of the retained species
V = L/tr
For Cu2+: V = 13.3cm/2172s
= 6.12 x 10-3 cms-1
For Fe2+: V = 13.3cm/2351s
= 5.66 x 10-3 cms-1
Calculation of Capacity (Retention) Factor
k’ = (tr – tm)/tm
For Cu2+: k’ = (2172 – 1564)/1564
= 0.389
For Fe2+: k’ = (2351 – 1564)/1564
= 0.503
Distribution constant
kA = (k’ x Vm)/Vs
For Cu2+: kA = (0.389 x 5.29)/6.25
= 0.329
For Fe2+: kA = (0.503 x 5.29)/6.25
= 0.426
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2. Selectivity Factor
a = kCu2+/kFe2+
= 0.329/0.426
= 0.77
Number of Plates in Column
N = 16 (tr/W)2
W = bandwidth
For Cu2+: W = 2351s – 1771s = 580s
N = 16 (2172/580)2
N = 224
For Fe2+: W = 2533s – 1985s = 548s
N = 16 (2351/548)2
N = 294
Height of each plate
H = L/N
For Cu2+: H = 13.3cm/224
= 0.059cm
For Fe2+: H = 13.3cm/294
= 0.045cm
Column Resolution
Rs = 2[(tr)Fe2+ - (tr)Cu2+]/(WFe2+ + WCu2+)
= 2 [2351s – 2172s]/(548s + 580s)
= 358/1128
= 0.317
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