Tecna Celer T2

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20 minutes ELISA test kit for the quantitative and sensitive detection of T2 and HT2 toxins in cereals and feed. For further informations, please visit Tecna website www.tecnalab.com.

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  • Visitate il nuovo sito www.tecnalab.it per maggiori informazioni sul kit ELISA Celer T2 cod. MT100 / MT101
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Tecna Celer T2

  1. 1. T2 T2 Fast immunoassay for the detection of T2 and HT2 toxins in cereals and feeda code MT 100 / MT101
  2. 2. FeaturesT2 Applications Standard curve example Cereals, feed Measuring range 0.025 - 1 ppm Detection limit Cereals, feed: 0.025 ppm Assay time 20 minutes Specificity T2 toxin 100% HT2 toxin 72%
  3. 3. Sample preparation T2Grinding, methanol extraction, filtration, dilution In details:Weight 50 g of homogeneous and ground sample. Add 10 g of NaCl. Add 250 ml Methanol-water solution 70:30. Shake 3 minutes. Filter. Dilute the extract 1:7 in water.
  4. 4. MAIZE validation T2 Specificity and Sensitivity 10 blank maize samples were analysed. The mean relative signal was 89±6%.By subtracting 2 standard deviations, a cut off was set at 76%, corresponding to 0.025 ppm. The same samples were spiked at 0.025 ppm T2 toxin. One determination up to twenty turned to be negative.The Limit of Quantification (LOQ) was set at 0.025 ppm. The sensitivity turned to be 95%. Summary Maize cut-off: 0.025 ppm Maize LOQ: 0.025 ppm
  5. 5. MAIZE validation T2 AccuracyA blank sample was spiked with T2 toxin in the range 0.1 - 1 ppm. The mean recovery was 109±13%. The graph on the right represents the linear correlation between spiked toxin concentration and results, including the results obtained at 0.025 ppm. Trueness Code T2 assigned value HT2 assigned value Sum and Celer T2 mean result satisfactory range (n=5) TC970 0.324 ppm 0.414 ppm 0.738 ppm 0.64±0.04 ppm (0.508-0.960 ppm) Satisfactory MTC999D 0.264 ppm 0.523 ppm 0.787 ppm 0.65±0.04 ppm (0.479-1.074 ppm) Satisfactory
  6. 6. WHEAT validation T2Specificity and Sensitivity 10 blank wheat samples were analysed. The mean relative signal was 84±5%.By subtracting 2 standard deviations, a cut off was set at 75%, corresponding to 0.026 ppm. The same samples were spiked at 0.025 ppm T2 toxin. All determinations were revealed as positive.The Limit of Quantification (LOQ) was set at 0.025 Summary ppm. Wheat cut-off: 0.026 ppm The sensitivity turned to be 100%. Wheat LOQ: 0.025 ppm
  7. 7. OATS validation T2Specificity and Sensitivity 10 blank oats samples were analysed. The mean relative signal was 79±8%.By subtracting 2 standard deviations, a cut off was set at 64%, corresponding to 0.030 ppm. The same samples were spiked at 0.050 ppm T2 toxin. All determinations were revealed as positive.The Limit of Quantification (LOQ) was set at 0.050 Summary ppm. Wheat cut-off: 0.030 ppm The sensitivity turned to be 100%. Wheat LOQ: 0.050 ppm
  8. 8. FEED validation T2 Specificity and Sensitivity 10 blank poultry feed samples were analysed. The mean relative signal was 76±8%. By subtracting 2 standard deviations, a cut off was set at 59%, corresponding to 0.047 ppm. The same samples were spiked at 0.050 ppm T2 toxin. All determinations were revealed as positive. The Limit of Quantification (LOQ) was set at 0.050 ppm. The sensitivity turned to be 100%. Summary10 blank samples of feed for pigs were implemented to study thepossible difference to the poultry ones. Their mean B/Bo was 77± Feed cut-off: 0.047 ppm11%. This experiment confirmed the cut-off at 59% B/Bo. Feed LOQ: 0.050 ppm
  9. 9. Advantages T2 sample preparation:The common sample extraction enabels end-users to extract sample just once andrun the same extract within all Celer test kits: Cereal sample 3 minutes methanol / water extraction Feed sample AFLA DON ZON FUMO T2 AFLA B1
  10. 10. Advantages T2Quickness: Sample preparation: < 10 minutes Assay time: 20 minutesHigh HT2 cross-reactivity: 72% cross-reactivity to HT2 Good accuracy on T2-HT2 incurred samplesDouble format available: Code: MT100 - 96 determinations Code MT101 - 48 determinations PLUS: easy concentration calculation with downloadable spreadsheet
  11. 11. More T2 Tecna’s port-folio of rapid and sensitive tools for mycotoxin screening in cerealsB ZEROfast ELISA test kits with no calibration curve for aflatoxin B1 and deoxynivalenolfast ELISA test kits for total aflatoxins, aflatoxin B1, fumonisins, zearalenone, deoxynivalenol, T2/HT2 toxinshigh sensitivity ELISA test kits for aflatoxins and ochratoxin A EZ FLOW New quantitative lateral flow for deoxynivalenol
  12. 12. Instruments and services Aquaculture Other foods More AllergensCereals Honey Dairy Feed Water Meat T2 TECNA CATALOGUE Find what best fits your screening necessities in different fields, for different analytes. Contact our team of experts for technical support Require our Performance Data Sheets to see our in-house validative results Download our spreadsheet for a fast and easy result calculation Contacts export@tecnalab.com servizioclienti@tecnalab.com

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