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1
EPISTATIC
INTERACTIONS
2
1. Complementary gene (9:7)
2. Duplicate gene (15:1)
3. Suppressor gene (13:3)
4. Additive gene (9:6:1)
5. Dominant epistasis (12:3:1)
6. Recessive epistasis (9:3:4)
Types
3
Complementary gene
(9:7)
 Production of one phenotype requires the presence of
dominant alleles of both the genes controlling the
character
C W
ccW
C ww
ccww
Contrasting phenotype
 Anyone of the two dominant gene is unable to produce the
phenotype when alone
 They complement each other to produce concerned
phenotype when they are together
4
5
Reginald Punnett (left) joined William Bateson (right) in 1903
6
Sweet pea (Lathyrus odoratus)
7
Duplicate gene
(15:1)
 Characters governed by duplicate gene action are
determined by two completely dominant genes
 Duplicate gene action: The presence of a single dominant
allele of any one of the two genes governing the trait
produce the dominant
phenotype
W
wwcc Contrasting phenotype
8
9
Endosperm colour in maize
10
Seed coat colour of oat
11
Floating habit of rice
12
Suppressor gene
(13:3)
 The gene don’t directly cause the expression of the
characters but suppress the expression of other genes
Example
Leaf colour of rice: Green Purple
Gg
I suppress G i
Ineffective
13
14
Leaf colour of rice
Additive gene/ Polymeric genes/
Duplicate gene with cumulative effect
9:6:1
15
Two completely dominant genes controlling a character
R W
R W
But both genes are present together, their phenotypic
effect is enhanced as the effect of the two genes
were cumulative additive
R W
16
It produces identical phenotypes when they are alone
17
Seed colour of wheat
18
R_B_
rrbb
R_bb
rrB_
Red colour in wheat is produced by the genotype R_B_, white
by the double recessive genotype, rrbb. The genotypes R_bb
and rrB_ produce brown kernels. If homozygous red variety
is crossed to a white variety what phenotypic results are
expected in the F1 and F2?
19
RB Rb rB rb
RB RRBB
Red
RRBb
Red
RrBB
Red
RrBb
Red
Rb RRBb
Red
RRbb
Brown
RrBb
Red
Rrbb
Brown
rB RrBB
Red
RrBb
Red
rrBB
Brown
rrBb
Brown
rb RrBb
Red
Rrbb
Brown
rrBb
Brown
rrbb
White
Punnett Square
20
RB Rb rB rb
RB RRBB
Red
RRBb
Red
RrBB
Red
RrBb
Red
Rb RRBb
Red
RRbb
Brown
RrBb
Red
Rrbb
Brown
rB RrBB
Red
RrBb
Red
rrBB
Brown
rrBb
Brown
rb RrBb
Red
Rrbb
Brown
rrBb
Brown
rrbb
White
Punnett Square
21
RB Rb rB rb
RB RRBB
Red
RRBb
Red
RrBB
Red
RrBb
Red
Rb RRBb
Red
RRbb
Brown
RrBb
Red
Rrbb
Brown
rB RrBB
Red
RrBb
Red
rrBB
Brown
rrBb
Brown
rb RrBb
Red
Rrbb
Brown
rrBb
Brown
rrbb
White
Punnett Square
22
RB Rb rB rb
RB RRBB
Red
RRBb
Red
RrBB
Red
RrBb
Red
Rb RRBb
Red
RRbb
Brown
RrBb
Red
Rrbb
Brown
rB RrBB
Red
RrBb
Red
rrBB
Brown
rrBb
Brown
rb RrBb
Red
Rrbb
Brown
rrBb
Brown
rrbb
White
Punnett Square for F2
Phenotypic ratio of F2 generation is Red: Brown: White = 9:6:1
23
Dominant epistasis
12:3:1
24
The two genes affecting the character W Y
When both genes are present in recessive state a
different phenotype is produced
They produce distinct phenotype when they are alone
W Y
But when the genes are present together, the expression
of one gene masks the expression of other
YW
w y
25
Fruits of Cucurbita pepa will be in green, yellow and white
colour. White is dominant over both yellow and green but
yellow is dominant over green only. White colour is
determined by the dominant gene W and no other gene for
fruit colour can be expressed in its presence. Thus
dominant W is epistatic to two other fruit colour. In the
presence of homozygous recessive, ww another gene Y
determine the yellow colour. Homozygous recessive plants
for both genes, wwyy bears green fruit that the classical
ratio the first two classes of a dihybrid ratio are
phenotypic ally similar
26
27
Fruit colour of Cucurbita pepa
wwyy
W_ _ _
ww Y_
28
When homozygous white fruit seeds are crossed with
homozygous green fruit seeds, the F1 is all white. Crossing
with F1 among themselves F2 produced 121 white, 28 yellow
and 9 green. Explain the results
29
WY Wy wY wy
WY WWYY
White
WWYy
White
WwYY
White
WwYy
White
Wy WWYy
White
WWyy
White
WwYy
White
Wwyy
White
wY WwYY
White
WwYy
White
wwYY
Yellow
wwYy
Yellow
wy WwYy
White
Wwyy
White
wwYy
Yellow
wwyy
Green
Punnett Square
30
WY Wy wY wy
WY WWYY
White
WWYy
White
WwYY
White
WwYy
White
Wy WWYy
White
WWyy
White
WwYy
White
Wwyy
White
wY WwYY
White
WwYy
White
wwYY
Yellow
wwYy
Yellow
wy WwYy
White
Wwyy
White
wwYy
Yellow
wwyy
Green
Punnett Square
31
WY Wy wY wy
WY WWYY
White
WWYy
White
WwYY
White
WwYy
White
Wy WWYy
White
WWyy
White
WwYy
White
Wwyy
White
wY WwYY
White
WwYy
White
wwYY
Yellow
wwYy
Yellow
wy WwYy
White
Wwyy
White
wwYy
Yellow
wwyy
Green
Punnett Square for F2
Phenotypic ratio of F2 generation is White: Yellow: Green = 12:3:132
33
Recessive epistasis
9:3:4
34
In this gene interaction, the dominant allele of one gene
produces a phenotypic effect
The dominant allele of the other gene does not produce any
phenotypic effect
But when it is present with dominant allele of the first
gene, it modifies the phenotypic effect produced by the
first gene
C A
C A
C ACA CA
35
Coat colour of mice
36
The coat colour of mice is control by two genes C and A
C alone determine black colour but due to supplementary
effect of A the colour is become black
C_A_
37
When only gene C is homozygous recessive albino mice
are produce
cc_ _
A alone has no effect
C_ aa
38
Mating between black mice of identical genotype produced
offspring as follows; 14 Gray, 47 Black and 19 Albino.
1. What epistastic ratio is approximated by these offspring?
2. What are the phenotypes of the parents and offspring?
39
CA Ca cA ca
CA CCAA
Black
CCAa
Black
CcAA
Black
CcAa
Black
Ca CCAa
Black
CCaa
Gary
CcAa
Black
Ccaa
Gray
cA CcAA
Black
CcAa
Black
ccAA
Albino
ccAa
Albino
ca CcAa
Black
Ccaa
Gray
ccAa
Albino
Ccaa
Albino
Punnett Square for F2
Phenotypic ratio is Black: Gray: Albino= 9: 3: 4
40
Summary of epistatic interaction
41
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Epistatic Interaction - 02 03-2015

  • 2. 2 1. Complementary gene (9:7) 2. Duplicate gene (15:1) 3. Suppressor gene (13:3) 4. Additive gene (9:6:1) 5. Dominant epistasis (12:3:1) 6. Recessive epistasis (9:3:4) Types
  • 4.  Production of one phenotype requires the presence of dominant alleles of both the genes controlling the character C W ccW C ww ccww Contrasting phenotype  Anyone of the two dominant gene is unable to produce the phenotype when alone  They complement each other to produce concerned phenotype when they are together 4
  • 5. 5 Reginald Punnett (left) joined William Bateson (right) in 1903
  • 8.  Characters governed by duplicate gene action are determined by two completely dominant genes  Duplicate gene action: The presence of a single dominant allele of any one of the two genes governing the trait produce the dominant phenotype W wwcc Contrasting phenotype 8
  • 13.  The gene don’t directly cause the expression of the characters but suppress the expression of other genes Example Leaf colour of rice: Green Purple Gg I suppress G i Ineffective 13
  • 15. Additive gene/ Polymeric genes/ Duplicate gene with cumulative effect 9:6:1 15
  • 16. Two completely dominant genes controlling a character R W R W But both genes are present together, their phenotypic effect is enhanced as the effect of the two genes were cumulative additive R W 16 It produces identical phenotypes when they are alone
  • 19. Red colour in wheat is produced by the genotype R_B_, white by the double recessive genotype, rrbb. The genotypes R_bb and rrB_ produce brown kernels. If homozygous red variety is crossed to a white variety what phenotypic results are expected in the F1 and F2? 19
  • 20. RB Rb rB rb RB RRBB Red RRBb Red RrBB Red RrBb Red Rb RRBb Red RRbb Brown RrBb Red Rrbb Brown rB RrBB Red RrBb Red rrBB Brown rrBb Brown rb RrBb Red Rrbb Brown rrBb Brown rrbb White Punnett Square 20
  • 21. RB Rb rB rb RB RRBB Red RRBb Red RrBB Red RrBb Red Rb RRBb Red RRbb Brown RrBb Red Rrbb Brown rB RrBB Red RrBb Red rrBB Brown rrBb Brown rb RrBb Red Rrbb Brown rrBb Brown rrbb White Punnett Square 21
  • 22. RB Rb rB rb RB RRBB Red RRBb Red RrBB Red RrBb Red Rb RRBb Red RRbb Brown RrBb Red Rrbb Brown rB RrBB Red RrBb Red rrBB Brown rrBb Brown rb RrBb Red Rrbb Brown rrBb Brown rrbb White Punnett Square 22
  • 23. RB Rb rB rb RB RRBB Red RRBb Red RrBB Red RrBb Red Rb RRBb Red RRbb Brown RrBb Red Rrbb Brown rB RrBB Red RrBb Red rrBB Brown rrBb Brown rb RrBb Red Rrbb Brown rrBb Brown rrbb White Punnett Square for F2 Phenotypic ratio of F2 generation is Red: Brown: White = 9:6:1 23
  • 25. The two genes affecting the character W Y When both genes are present in recessive state a different phenotype is produced They produce distinct phenotype when they are alone W Y But when the genes are present together, the expression of one gene masks the expression of other YW w y 25
  • 26. Fruits of Cucurbita pepa will be in green, yellow and white colour. White is dominant over both yellow and green but yellow is dominant over green only. White colour is determined by the dominant gene W and no other gene for fruit colour can be expressed in its presence. Thus dominant W is epistatic to two other fruit colour. In the presence of homozygous recessive, ww another gene Y determine the yellow colour. Homozygous recessive plants for both genes, wwyy bears green fruit that the classical ratio the first two classes of a dihybrid ratio are phenotypic ally similar 26
  • 27. 27 Fruit colour of Cucurbita pepa
  • 28. wwyy W_ _ _ ww Y_ 28
  • 29. When homozygous white fruit seeds are crossed with homozygous green fruit seeds, the F1 is all white. Crossing with F1 among themselves F2 produced 121 white, 28 yellow and 9 green. Explain the results 29
  • 30. WY Wy wY wy WY WWYY White WWYy White WwYY White WwYy White Wy WWYy White WWyy White WwYy White Wwyy White wY WwYY White WwYy White wwYY Yellow wwYy Yellow wy WwYy White Wwyy White wwYy Yellow wwyy Green Punnett Square 30
  • 31. WY Wy wY wy WY WWYY White WWYy White WwYY White WwYy White Wy WWYy White WWyy White WwYy White Wwyy White wY WwYY White WwYy White wwYY Yellow wwYy Yellow wy WwYy White Wwyy White wwYy Yellow wwyy Green Punnett Square 31
  • 32. WY Wy wY wy WY WWYY White WWYy White WwYY White WwYy White Wy WWYy White WWyy White WwYy White Wwyy White wY WwYY White WwYy White wwYY Yellow wwYy Yellow wy WwYy White Wwyy White wwYy Yellow wwyy Green Punnett Square for F2 Phenotypic ratio of F2 generation is White: Yellow: Green = 12:3:132
  • 34. 34 In this gene interaction, the dominant allele of one gene produces a phenotypic effect The dominant allele of the other gene does not produce any phenotypic effect But when it is present with dominant allele of the first gene, it modifies the phenotypic effect produced by the first gene C A C A C ACA CA
  • 36. 36 The coat colour of mice is control by two genes C and A C alone determine black colour but due to supplementary effect of A the colour is become black C_A_
  • 37. 37 When only gene C is homozygous recessive albino mice are produce cc_ _ A alone has no effect C_ aa
  • 38. 38 Mating between black mice of identical genotype produced offspring as follows; 14 Gray, 47 Black and 19 Albino. 1. What epistastic ratio is approximated by these offspring? 2. What are the phenotypes of the parents and offspring?
  • 39. 39 CA Ca cA ca CA CCAA Black CCAa Black CcAA Black CcAa Black Ca CCAa Black CCaa Gary CcAa Black Ccaa Gray cA CcAA Black CcAa Black ccAA Albino ccAa Albino ca CcAa Black Ccaa Gray ccAa Albino Ccaa Albino Punnett Square for F2 Phenotypic ratio is Black: Gray: Albino= 9: 3: 4
  • 40. 40 Summary of epistatic interaction