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MIKROBIOLOGI
   MIKROBIOLOGI

          OLEH :
  KHIKI PURNAWATI KASIM
STERILISASI

 TUJUAN :
Memusnahkan semua MO baik bentuk vegetatif
maupun spora


STERIL : Suatu keadaan dimana objek atau benda yang disterilisasi
         sudah berada dalam kondisi suci hama.



DESINFEKSI : Sterilisasi menggunakan bahan kimia
A. PANAS
  - PANAS KERING
   UNTUK ALAT-ALAT YANG TERBUAT DARI GELAS JUGA
   DARI
   KAWAT LOGAM
    1. MELIDAH APIKAN (FLAMING)
    2. MEMIJARKAN (RED HEAT)
    3. OVEN

 - PANAS BASAH
   1. DI BAWAH 100OC (PASTEURISASI)
   2. 1000C (WATER BATH)
   3. DI ATAS 100OC (AUTOCLAVE)
B. RADIASI
             MIKROBIOLOGI
  -SINAR ULTRAVIOLET
  -SINAR GAMMA
  -SINAR X
  -SINAR KATODA


C. FILTRASI            OLEH :
          KHIKI PURNAWATI KASIM
   TIPE FILTER/SARINGAN :
   -BERKEFELD FILTER DAN CHAMBERLAND FILTER
    Terbuat dari porselin dengan ukuran pori :
     1. Veil/Kasar (V)
     2. Wenig/Halus (W)
     3. Normal (N)
   -SEITZ FILTER
     Terbuat dari komposisi asbes,ukuran sda
   -SINTERED GLASS FILTER
    Terbuat dari gelas untuk menyaring cairan
   -CELULOSE MEMBRAN FILTER
     Dilengkapi dengan membran/selaput selulosa nitrat/acetat
MIKROBIOLOGI
BAHAN DESINFEKTAN YANG DIGUNAKAN :
-CHLOROFORM digunakan pada serum dan bentuk vegetatif dari
                bakteri
-PHENOL : LISOL
-GARAM LOGAM BERAT : MERCURY CHLORIDA
                  OLEH :
-FORMALDEHIDA :LARUTAN DAN GAS
          KHIKI PURNAWATI KASIM
-ETILEN OKSIDA :GAS
-PERSENYAWAAN ALKOHOL
-GOLONGAN HALOGEN : CHLOR(CaHOCl2) DAN YODIUM
Sterilisasi

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Sterilisasi

  • 1. MIKROBIOLOGI MIKROBIOLOGI OLEH : KHIKI PURNAWATI KASIM
  • 2. STERILISASI TUJUAN : Memusnahkan semua MO baik bentuk vegetatif maupun spora STERIL : Suatu keadaan dimana objek atau benda yang disterilisasi sudah berada dalam kondisi suci hama. DESINFEKSI : Sterilisasi menggunakan bahan kimia
  • 3. A. PANAS - PANAS KERING UNTUK ALAT-ALAT YANG TERBUAT DARI GELAS JUGA DARI KAWAT LOGAM 1. MELIDAH APIKAN (FLAMING) 2. MEMIJARKAN (RED HEAT) 3. OVEN - PANAS BASAH 1. DI BAWAH 100OC (PASTEURISASI) 2. 1000C (WATER BATH) 3. DI ATAS 100OC (AUTOCLAVE)
  • 4. B. RADIASI MIKROBIOLOGI -SINAR ULTRAVIOLET -SINAR GAMMA -SINAR X -SINAR KATODA C. FILTRASI OLEH : KHIKI PURNAWATI KASIM TIPE FILTER/SARINGAN : -BERKEFELD FILTER DAN CHAMBERLAND FILTER Terbuat dari porselin dengan ukuran pori : 1. Veil/Kasar (V) 2. Wenig/Halus (W) 3. Normal (N) -SEITZ FILTER Terbuat dari komposisi asbes,ukuran sda -SINTERED GLASS FILTER Terbuat dari gelas untuk menyaring cairan -CELULOSE MEMBRAN FILTER Dilengkapi dengan membran/selaput selulosa nitrat/acetat
  • 5. MIKROBIOLOGI BAHAN DESINFEKTAN YANG DIGUNAKAN : -CHLOROFORM digunakan pada serum dan bentuk vegetatif dari bakteri -PHENOL : LISOL -GARAM LOGAM BERAT : MERCURY CHLORIDA OLEH : -FORMALDEHIDA :LARUTAN DAN GAS KHIKI PURNAWATI KASIM -ETILEN OKSIDA :GAS -PERSENYAWAAN ALKOHOL -GOLONGAN HALOGEN : CHLOR(CaHOCl2) DAN YODIUM