Traditional design methods for flow cytometers and other complex biophotonic systems are increasingly recognized as a major bottleneck in instrumentation development. The many manual steps involved in the analysis and translation of the design, from optical layout to a detailed mechanical model and ultimately to a fully functional instrument, are labor-intensive and prone to wasteful trial-and-error iterations. We have developed two complementary, linked technologies that address this problem: one design tool (LiveIdeas™) provides an intuitive environment for interactive, real-time simulations of system-level performance; the other tool (BeamWise™) automates the generation of mechanical 3D CAD models based on those simulations. The strength of our approach lies in a parametric modeling strategy that breaks boundaries between engineering subsystems (e.g., optics and fluidics) to predict critical behavior of the instrument as a whole. The results: 70 percent reduction in early-stage project effort, significantly enhancing the probability of success by virtue of a more efficient exploration of the design space.

1. March 20, 20141©
2014
Design
Parametrics,
Incorporated,
Kine9c
River
Corpora9on,
Plan
Energy
Ltd.
www.BeamWise.net
Advances
in
Op-cal
Design
OSA,
Northern
California
Sec9on
March
20,
2014
Giacomo
Vacca,
Ph.D.1,
Hannu
Leh9mäki2,
Tapio
Karras3,
Sean
Murphy4
1Kine9c
River
Corp.,
2Plan
energy
Ltd.,
3Design
Parametrics
Inc.,
4SKMurphy
Inc.

2. March 20, 20142©
2014
Design
Parametrics,
Incorporated,
Kine9c
River
Corpora9on,
Plan
Energy
Ltd.
www.BeamWise.net

3. March 20, 20143©
2014
Design
Parametrics,
Incorporated,
Kine9c
River
Corpora9on,
Plan
Energy
Ltd.
www.BeamWise.net

4. March 20, 20144©
2014
Design
Parametrics,
Incorporated,
Kine9c
River
Corpora9on,
Plan
Energy
Ltd.
www.BeamWise.net

5. March 20, 20145©
2014
Design
Parametrics,
Incorporated,
Kine9c
River
Corpora9on,
Plan
Energy
Ltd.
www.BeamWise.net
Semrock’s
SearchLight™

6. March 20, 20146©
2014
Design
Parametrics,
Incorporated,
Kine9c
River
Corpora9on,
Plan
Energy
Ltd.
www.BeamWise.net
Example:
Flow
Cytometry
© 2011 Life Tech
light
source(s)
flow
cell
forward-­‐sca[er
detector(s)
fluorescence
detector(s)
side-­‐sca[er
detector(s)
spectral
filters

7. March 20, 20147©
2014
Design
Parametrics,
Incorporated,
Kine9c
River
Corpora9on,
Plan
Energy
Ltd.
www.BeamWise.net

8. March 20, 20148©
2014
Design
Parametrics,
Incorporated,
Kine9c
River
Corpora9on,
Plan
Energy
Ltd.
www.BeamWise.net
New
Design
Tools:
LiveIdeas
and
BeamWise

9. March 20, 20149©
2014
Design
Parametrics,
Incorporated,
Kine9c
River
Corpora9on,
Plan
Energy
Ltd.
www.BeamWise.net
LiveIdeas™:
Op9mize
Design

10. March 20, 201410©
2014
Design
Parametrics,
Incorporated,
Kine9c
River
Corpora9on,
Plan
Energy
Ltd.
www.BeamWise.net
BeamWise™
Automa9ng
Biophotonics
System
Design
•
Beam-­‐anchored
tool
•
Builds
3D
CAD
model
using:
– simulator
results
– func9onal
layout
– optomechanical
component
data
•
Creates
2D
drawings
&
parts
lists
for
manufacturing
•
Supports
explora9on
of
design
op9ons
with
efficient
change
management

11. March 20, 201411©
2014
Design
Parametrics,
Incorporated,
Kine9c
River
Corpora9on,
Plan
Energy
Ltd.
www.BeamWise.net
BeamWise™:
Automated
3D
Model
&
Document
Crea9on

12. March 20, 201412©
2014
Design
Parametrics,
Incorporated,
Kine9c
River
Corpora9on,
Plan
Energy
Ltd.
www.BeamWise.net
Step-­‐by-­‐Step
Walkthrough

13. March 20, 201413©
2014
Design
Parametrics,
Incorporated,
Kine9c
River
Corpora9on,
Plan
Energy
Ltd.
www.BeamWise.net

14. March 20, 201414©
2014
Design
Parametrics,
Incorporated,
Kine9c
River
Corpora9on,
Plan
Energy
Ltd.
www.BeamWise.net
Network(
Path(ID
NetList(
Vector
From To Magnitude
Mag.(
Units
Direction
Dir.(
Units
B1 v01 SO01 MI01 50.00++++++++++++ mm 270 degrees
B1 v02 MI01 LE01 50.00++++++++++++ mm 180 degrees
B1 v03 LE01 LE02 50.00++++++++++++ mm 180 degrees
B1 v04 LE02 LE03 150.00++++++++++ mm 180 degrees
B1 v05 LE03 MI02 50.00++++++++++++ mm 180 degrees
B1 v06 MI02 LE04 75.00++++++++++++ mm 90 degrees
B1 v07 LE04 LE05 50.00++++++++++++ mm 90 degrees
B1 v08 LE05 FC01 75.00++++++++++++ mm 90 degrees
B1 v09 FC01 LE06 50.00++++++++++++ mm 90 degrees
B1 v10 LE06 PD01 50.00++++++++++++ mm 90 degrees
(B2,+B3) v11 FC01 MO01 38.00++++++++++++ mm 0 degrees
(B2,+B3) v12 MO01 LE07 65.00++++++++++++ mm 0 degrees
(B2,+B3) v13 LE07 BS01 70.00++++++++++++ mm 0 degrees
B2 v14 BS01 FI01 25.00++++++++++++ mm 0 degrees
B2 v15 FI01 FI02 35.00++++++++++++ mm 0 degrees
B2 v16 FI02 PD02 25.00++++++++++++ mm 0 degrees
B3 v17 BS01 FI03 25.00++++++++++++ mm 270 degrees
B3 v18 FI03 FI04 35.00++++++++++++ mm 270 degrees
B3 v19 FI04 PD03 25.00++++++++++++ mm 270 degrees

15. March 20, 201415©
2014
Design
Parametrics,
Incorporated,
Kine9c
River
Corpora9on,
Plan
Energy
Ltd.
www.BeamWise.net
3D
View

16. March 20, 201416©
2014
Design
Parametrics,
Incorporated,
Kine9c
River
Corpora9on,
Plan
Energy
Ltd.
www.BeamWise.net

17. March 20, 201417©
2014
Design
Parametrics,
Incorporated,
Kine9c
River
Corpora9on,
Plan
Energy
Ltd.
www.BeamWise.net

18. March 20, 201418©
2014
Design
Parametrics,
Incorporated,
Kine9c
River
Corpora9on,
Plan
Energy
Ltd.
www.BeamWise.net
2D
Layout

19. March 20, 201419©
2014
Design
Parametrics,
Incorporated,
Kine9c
River
Corpora9on,
Plan
Energy
Ltd.
www.BeamWise.net

20. March 20, 201420©
2014
Design
Parametrics,
Incorporated,
Kine9c
River
Corpora9on,
Plan
Energy
Ltd.
www.BeamWise.net

21. March 20, 201421©
2014
Design
Parametrics,
Incorporated,
Kine9c
River
Corpora9on,
Plan
Energy
Ltd.
www.BeamWise.net

22. March 20, 201422©
2014
Design
Parametrics,
Incorporated,
Kine9c
River
Corpora9on,
Plan
Energy
Ltd.
www.BeamWise.net

23. March 20, 201423©
2014
Design
Parametrics,
Incorporated,
Kine9c
River
Corpora9on,
Plan
Energy
Ltd.
www.BeamWise.net
BeamWise™:
Automated
3D
Model
&
Document
Crea9on

24. March 20, 201424©
2014
Design
Parametrics,
Incorporated,
Kine9c
River
Corpora9on,
Plan
Energy
Ltd.
www.BeamWise.net
BeamWise™
Automa9ng
Biophotonics
System
Design
• Kine9c
River
– system
design
• Design
Parametrics
– CAD
modeling
plaborm
• PlanEnergy
– tool
development
• SKMurphy
– concept,
biz
development

25. March 20, 201425©
2014
Design
Parametrics,
Incorporated,
Kine9c
River
Corpora9on,
Plan
Energy
Ltd.
www.BeamWise.net
Kine9c
River:
What
ELSE
We
Do

26. March 20, 201426©
2014
Design
Parametrics,
Incorporated,
Kine9c
River
Corpora9on,
Plan
Energy
Ltd.
www.BeamWise.net
Giacomo
Vacca,
Ph.D.
• Kine-c
River
(founder)
• Abbo>
Labs
(Res.
Fellow)
• Picarro
• Lightwave
Micro
• Exxon
Research
&
Engr.
• 28
patents
issued/pending

27. March 20, 201427©
2014
Design
Parametrics,
Incorporated,
Kine9c
River
Corpora9on,
Plan
Energy
Ltd.
www.BeamWise.net
Kine9c
River
Mission
Kine9c
River
is
a
biophotonics
consul9ng
and
product
development
company
with
a
passion
for
crea9ng
and
enabling
tools
for
innova9ve
and
disrup9ve
life
science
research,
biomedical
instrumenta9on,
and
clinical
diagnos9cs.
Where
Light
Meets
Life™

28. March 20, 201428©
2014
Design
Parametrics,
Incorporated,
Kine9c
River
Corpora9on,
Plan
Energy
Ltd.
www.BeamWise.net
Danube
I:
1st-­‐Gen
FLT
Flow
Cytometer
• Full
custom
build
• Collab.
with
New
Mexico
State
U.
• Concept,
design,
build,
test,
support
• Op9cs,
microfluidics,
control
SW
•
20-­‐ns
light-­‐cell
interac9on
9me
2-­‐ns
life-me
resolu-on

29. March 20, 201429©
2014
Design
Parametrics,
Incorporated,
Kine9c
River
Corpora9on,
Plan
Energy
Ltd.
www.BeamWise.net
Kine9c
River
Clients
• Beckman
Coulter
(part
of
Danaher)
• Dolomite
Microfluidics
• EMD
Millipore
(part
of
Merck
KGaA)
• Gerson
Lehrman
Group
• NanoCellect
• Na9onal
Instruments
• Houston
Lab
(New
Mexico
State
University)
• Na9onal
Cancer
Ins9tute
(NIH)
• Product
Realiza9on
Group
• Nolan
Lab
(Stanford
School
of
Medicine)
• TempSync
• [others]

30. March 20, 201430©
2014
Design
Parametrics,
Incorporated,
Kine9c
River
Corpora9on,
Plan
Energy
Ltd.
www.BeamWise.net
Kine9c
River
Key
Exper9se
System
Domain
Knowledge
System
Design
Microfluidics
2D
/
3D
CAD
Op9cs
LiveIdeas™
BeamWise™