This document provides information about Enzo Life Sciences, a company that provides products and services for life sciences research, drug development, and clinical research. It summarizes Enzo's product offerings which include reagents, assays, cell analysis tools, small molecule libraries, and genomics products. It also describes Enzo's mission to develop novel platforms that enable researchers to find better solutions for disease research and drug development.

1. Life Sciences
Biochem
®
Life Sciences Research
Drug Development
Clinical Research
scientists enabling scientists.
ENZO LIFE SCIENCES

2. Global Research
Our strong roots in the life sciences industry extend beyond the laboratory. We
understand the importance of delivering product to you quickly and efficiently.
Our website, www.enzolifesciences.com, offers our global customers easy access
to thousands of unique products with enhanced search functionality and secure
online ordering. Our distributor sister site, www.axxora.com, was one of the original
e-commerce websites and continues to provide a “one-stop” marketplace for a
multitude of products from a variety of suppliers and manufacturers.
Enzo Biochem (NYSE:ENZ)
We are an important segment of our parent company, Enzo Biochem, a vertically
integrated clinical lab, life sciences, and therapeutic biotechnology company. We use
the technology and knowledge of our sister divisions to support the innovation driving
new product platform development. Links to these divisions provide ready access
to difficult-to-procure clinical samples, facilitating thorough product validation from
concept to market launch. A continual voice from the clinical market keeps us tied to
current needs,helping to define our new product pipeline and maximize our R&D efforts.
From Our Bench To Yours
For over 30 years, Enzo has been providing enabling technologies for the research,
development, manufacture, licensing, and marketing of innovative life science and
health care products and services based on molecular and cellular technologies.
Our Mission
We strive to develop novel platforms that enable researchers to find better, more
reliable solutions throughout the drug development pipeline and clinical research
laboratory.Through this journey, we hope not only to connect with our customers, but
to enable a future with more hope, more collaboration, and less disease.
Collective Strength
Working with our customers,we create unique solutions focused on the Life Sciences
Research, Drug Development, and Clinical Research markets. Through our recent
acquisitions of Alexis®
Biochemicals, Biomol International, Assay Designs®
, and
Stressgen®
Bioreagents,we have aligned the skills and scientific expertise associated
with these industry-established brands. With custom developed solutions and off-
the-shelf catalog products, we provide the life sciences market with a unique array
of fluorescent probes, dyes, and labeling systems for molecular biology and cellular
analysis, as well as a broad range of bioreagents including antibodies, proteins, small
molecules, ELISA kits, and enzyme assays.
Our foundation rests in the collective strength of our experience, unique technology platforms and thousands of peer-reviewed literature citations.
2 3
Enzo Biochem
Enzo
Therapeutics
Enzo
Clinical Labs
Enzo
Life Sciences
Life Sciences Research
Drug Discovery
Clinical Research

3. Your goal is our goal
Whether your focus is disease research, target identification and
validation, process monitoring, or diagnostic test development, our
products work for you. Our breadth of scientific expertise coupled with
unique technology platforms give us the flexibility to drive innovation
throughout the entire research continuum. From life sciences research
discovery efforts, through drug development, and into clinical research,
our comprehensive range of products are designed to support your
goals. When a standard catalog product does not meet your needs, talk
to us about custom solutions; we can help.
INTEGRATED SOLUTIONS
FOR IMPROVING HUMAN HEALTH
DRUG DEVELOPMENT
LIFE SCIENCES RESEARCH
CLINICAL RESEARCH
4 5

4. LIFE SCIENCES RESEARCH
ENABLING disease research
Major disease research programs depend upon pursuit of a
comprehensive understanding of the cellular and molecular basis of
disease and response to potential therapeutics. Our wide range of
technology platforms facilitate analysis at the gene, protein, or cellular
level. Our scientific expertise is enhanced by active participation
and collaboration with leaders in the areas of molecular biology,
proteostasis, and cell biology. We understand the etiology of clinically
relevant diseases including cancer, neurodegeneration, autoimmune
disease, diabetes, obesity, and more. We support basic discovery in
the fields of protein synthesis, folding, degradation, gene expression,
cell death pathways, and cellular responses to stress. Our portfolio of
over 9,000 assay kits, dyes, labels, small molecules, antibodies, and
proteins are reliable, extensively validated, and backed by thousands of
publications in peer-reviewed journals.
Advancements in diagnosis and treatment of disease stem from the early research efforts of scientists working towards a common goal —find the cause of a disease at the gene, protein, or cellular level.
eFluxx-ID®
Multidrug Resistance Assay
Stressgen®
HSP90 ELISA Kits & Antibodies
Screen-Well®
FDA Approved Drugs, Natural
Products & Protease Inhibitor Libraries
HDAC & Sirtuin Activity Assays
Nuclear-ID®
Cell Cycle Assays
aCGH Labeling Kits
Screen-Well®
Wnt Pathway Library & Wnt Proteins
Autophagy Cell Analysis & ELISA Kits
Cancer
Corticosterone, Testosterone, & Estradiol ELISA Kits
Oxytocin/Vasopressin ELISA Kits
Screen-Well®
Receptor De-orphaning Libraries
Endocrine Disorders
Hepatotoxicity
M30-Apoptosense®
ELISA Kit
M65-EpiDeath®
ELISA Kit
Creatinine Detection Kit
NGAL ELISA Kits
Renal Injury
Infectious & Autoimmune Disease
Eicosanoid & Cytokine ELISA Kits
MBL Oligomer Assay
PathoGene®
HPV Probes
Neurological Disorders
ProteoStat®
Amyloid Plaque Detection Kit
ProteoStat®
Aggresome Detection Kit
SMN ELISA Kit
Ubiquitin & Proteasome Detection Kits & Reagents
Stressgen®
HSP70 ELISA Kits & Antibodies
Cyto-ID®
Autophagy Kit
Diabetes & Obesity
GLP-1 & Exedin-4 Antibodies
DPPIV/CD26 Activity Assay
Adipokines
Insulin Signaling
PI3K/mTOR/Akt Pathway Kits & Reagents
Cardiovascular Disease
Angiotensin ELISA Kits
Bradykinin ELISA Kit
MMP Drug Discovery Assays
Cyclic GMP ELISA Kits
Nitric Oxide Detection Assays
Mitochondrial Activity Kits
Screen-Well®
Wnt Pathway Library & Wnt Proteins
EXPLORE DISEASE FROM MANY ANGLES
6 7

5. DRUG DEVELOPMENT
enabling solutions across the drug
DEVELOPMENT pipeline
Development of a single drug, whether it is a new chemical entity or
a biological therapeutic, requires significant investment of resources.
Each step of the process from early discovery through production and
delivery must be fully explored, characterized, and understood.
Enzo Life Sciences continues to expand its offering of products for
all drug development initiatives with an emphasis on secondary
screening, in vitro toxicology assays, and bioprocess optimization.
The unique needs of each are considered during the development and
characterization of every Enzo product. Our aim is to deliver tools that
make drug development more efficient, more cost-effective, and more
successful.
Every blockbuster drug or diagnostic test was borne through solid science as well as diligence in safety testing, scale-up optimization and robust manufacturing.
Drug
Development
Pipeline
Biotherapeutics
Development
Process
Design
Formulation
Manufacturing
Quality Control
Delivery
MOVE FORWARD
WITH MORE CERTAINTY
8 9
Target ID
Assay Development
Primary Screen
Secondary Screen
Hit-to-Lead
Lead Optimization
Pre-Clinical
Clinical Phase 1
Clinical Phase 2
Clinical Phase 3
Immunoassays
Cell Based Assays
Antibodies & Proteins
Compound Libraries
Chemical Sourcing
In Vitro Toxicity Assays
Enzyme Activity Assays
FluoroForte®
Calcium Assay Kits
p62 & NBR1 ELISA Kits
Stressgen®
HSP70 High Sensitivity ELISA Kit
24(S)-Hydroxycholesterol ELISA Kit
BioPorto®
MBL Oligomer Assay
BioPorto®
NGAL ELISA Kits
PEGylated Protein ELISA Kit
Protein A ELISA Kit
ProteoStat®
Aggregation Assays
Custom Assays & Affinity Resins
Custom Assay Development
PEG ELISA Kit
Protein A ELISA Kit
ProteoStat®
Aggregation Assays

6. CLINICAL RESEARCH
validated products enable
clinical research testing
Enzo Life Sciences has more than 30 years of experience in the
development of enabling platform technologies, many of which provide
the foundation for widely used clinical research assays. Furthermore,
we have assembled considerable expertise in life science reagent
development, including molecular biology reagents, immunoassays,
activity assays, and small molecules, all of which serve the drug
development and clinical research areas, including biomarker research.
The unique integrated structure of Enzo Life Sciences, Enzo Clinical
Labs, and Enzo Therapeutics, under the Enzo Biochem umbrella, allows
for a multiplicity of approaches to capitalize on the clinical research
space. Our connection with Enzo Clinical Labs, a CAP-certified and New
York State permitted clinical reference laboratory, provides invaluable
insight into clinical research.
Clinical research is reliant on real-world testing and validated tools to assure consistent, repeatable results.
Enzo
Life Sciences
BROAD REACH FOR
COMPREHENSIVE SUPPORT
Enzo
Therapeutics
Enzo
Clinical Labs
Esoteric & Routine Services
Companion DiagnosticsDrug Candidates for Partnering
targ
ets
esot
erictests
screen
ingassays
clinicalsamp
les
10 11
Research Products
ELISA Kits for Biomarker Detection
Enzyme Activity Assays
Primary & Secondary Screening Assays
Compound Libraries
Custom Assay Development
Genomic Labeling & Amplification Kits

7. COMMITTED TO PROVIDING SOLUTIONS TO
ENABLE COMPREHENSIVE RESEARCH
CELLULAR ANALYSIS
GENOMICS
SMALL MOLECULE CHEMISTRY
PROTEOSTASIS & EPIGENETICS
IMMUNOASSAYS & ASSAY DEVELOPMENT
CUSTOM SERVICES
VARIED YET COMPLEMENTARY PLATFORMS
ENABLE INTEGRATED SOLUTIONS
Searching for answers to focused questions often draws on a variety of
research disciplines to look at the question from multiple angles. Using
our broad technology acumen, we offer comprehensive solutions within
important niche areas to enable this process.
Our core areas of scientific expertise lie within genomics, cellular
analysis, small molecule chemistry, proteostasis, epigenetics,
immunoassays, and assay development. Combined, the breadth of our
scientific expertise and manufacturing capabilities provide you with the
flexibility and continuity needed to accelerate your research. Although
all of our research tools are built to stand on their own, they are not
conceived this way, but rather as part of a greater collection that can
be used toward a specific area of discovery.
12 13

8. Genomic analysis of DNA extracted from formalin-fixed, paraffin-embedded (FFPE) tissue samples using array CGH
requires a significant investment in time and resources that can be wasted if sample DNA quality is poor. We provide
tools to determine the suitability of your genomic DNA sample, and our high-fidelity labeling kits ensure you can
identify aberrations in gene copy number with confidence.
aCGH Analysis
Identification of
Genomic Aberrations
DNA Labeling
Proprietary Cy-3 & Cy-5
Labeling Kits for Oligo Arrays
DNA Quality Assessment
BioScore®
Screening &
Amplification Kit
CLEAR RESULTS WITH INNOVATIVE
NUCLEIC ACID LABELING
GENOMICS
Enzo Life Sciences has long been recognized as a leading innovator of
tools for genomics research and development.The pillar of our molecular
biology portfolio is our DNA/RNA labeling chemistries that are applied to
array comparative genomic hybridization (aCGH) analysis. A powerful
tool for detecting DNA copy number gains and losses associated with
chromosome abnormalities, aCGH provides a greater understanding
and characterization of genetic disorders, cancers, and other genomic
aberrations. The proprietary labeling technology and high-performance
dyes incorporated into our aCGH kits deliver superior performance on
commonly utilized platforms such as Agilent®
arrays.
Supporting our aCGH kits are a variety of everyday-use molecular
biology products designed to maximize the quantity and quality of data
generated from your valuable samples. These include RNA and DNA
amplification kits, as well as labeling systems and modified nucleotides
designed for creating biotin- or fluorophore-labeled nucleic acid probes
for a variety of applications and detection platforms. The products
have been specifically designed to give opitmal performance with, for
example, nick translation or Agilent®
arrays.
Our panel of PathoGene®
kits provides high-specificity probes used to
classify human papillomavirus (HPV) genotypes in tissue sections by
in situ hybridization. Flexible SimplySensitive®
and UltraSensitive®
detection systems are optimized for use with biotin-labeled probes for
in situ detection of specific endogenous or pathogen-expressed genes.
14 15
DNA
HPV Infection
Viral DNA
Ribosome
Information
Information
Information
Replication
DNA Duplicates
mRNA
Protein
Genomic Labeling & Amplification
CGH Labeling Kit for Oligonucleotide Arrays
BioScore®
Screening & Amplification Kits
Modified Nucleotides
Cy3 & Cy5 dUTPs
Biotin Labeled Nucleotides
Non-radioactive Labeling Systems
BioArray®
Single Round RNA
Amplification& Labeling System
In situ Hybridization
SimplySensitive®
& UltraSensitive®
HRP & AP Detection Systems
HPV Detection System
PathoGene®
HPV Screening Probes & Assays

9. VISUALIZE CELLULAR RESPONSES
WITH NOVEL FLUORESCENT PROBES
CELLULAR
ANALYSIS
Our CELLestial®
portfolio of fluorescent probes and assay kits for cellular
analysis provides a complete set of tools for monitoring cell viability,
proliferation, death, oxidative stress, and ADME/Tox by flow cytometry,
microscopy, and microplate platforms.
Our photostable fluorescent probes are developed to maximize
compatibility with common counterstains and fluorescent marker-
expressingcelllines. CELLestial®
dyesallowpharmaceuticalandbiotech
researcherstoacceleratetheirpre-clinicalandclinicaldrugdevelopment
programs through early lead compound identification, lead candidate
selection, predictive toxicology, and compound characterization.
More than 300 assay kits and over 3,000 highly characterized antibodies
support further dissection of these responses. Custom formulation and
bulk production of antibodies are also available to fit your specific needs.
Apoptosis/Necrosis
Apoptosis/Necrosis Detection Kit
Nuclear-ID™ Chromatin Condensation Detection Kit
Calcium Mobilization
FluoForte®
Calcium Detection Kits
Cell Cycle Analysis
Nuclear-ID™ Cell Cycle Kits
Nucleolar-ID™ Detection Kits
Oxidative Stress
Mito-ID®
Membrane Potential Assay Kits
ROS/Superoxide Detection Kit
ROS/RNS Detection Kit
Hydrogen Peroxide Detection Kit
Nitric Oxide Detection Kit
Organelle Detection
ER-ID™ Assay Kits
Lyso-ID®
Detection Kits
Mito-ID®
Detection Kit
Nuclear-ID®
Cell Viability Reagents
Organelle-ID RGB™ Reagents
Multidrug Resistance
eFluxx-ID®
Multidrug Resistance Assay Kits
Cell Lineage Tracing
Cyto-ID®
Long-term Tracer Kits
Aggresome & Inclusion Body Detection
ProteoStat®
Aggresome Detection Kit
Autophagy
Cyto-ID®
Autophagy Detection Kit
Lyso-ID®
Cytotoxicity & Detection Kits
ProteoStat®
Aggresome Detection Kit
16 17

10. Chemical Genomics
ICCB Known Bioactives
Kinase Inhibitors
Phosphatase Inhibitors
Protease Inhibitors
Ion Channel Ligands
REDOX Compounds
Receptor De-Orphaning
Bioactive Lipids
Endocannabinoids
Fatty Acids
Neurotransmitters
Nuclear Receptor Ligands
Orphan Ligands
Drug Repurposing
FDA Approved Drug Library
Natural Products
Natural Products
Pathway Targeting
Autophagy
Epigenetics
Wnt Pathway
Screen-Well®
Focused Compound Libraries
Choose from Over 3,000 Compounds
Bulk/Custom Sourcing of Compounds
Infinite Possibilities for Customization
CAREFULLY CURATED LIBRARIES
STREAMLINE YOUR SCREENING PROCESS
SMALL MOLECULE
CHEMISTRY
Natural Products
Enzyme inhibitors
Receptor Ligands
Drugs
Lipids
Fatty Acids
Antagonists
... and much more
Enzo Life Sciences has a long and successful track record in identifying,
synthesizing, and commercializing valuable known bioactives for use as
research tools.
Our long-standing, flagship Screen-Well®
Compound Library product
family offers an easy, ready-to-use method for compound screening.
We have a unique offering of focused compound libraries including FDA-
approved drugs, natural products, receptor de-orphaning, chemical
genomics, and pathway targeting.
Our chemistry and compound range includes over 3,000 stand-alone
small molecules with known activity including natural products, enzyme
inhibitors, receptor ligands, drugs, lipids, and fatty acids.
Our staff of organic chemists are experienced in diverse synthetic
methods and techniques. We welcome requests for custom services
including development of new synthetic routes for novel molecules and
rapid sourcing of compounds.
Through our supplier network, built over a 25 year history, we have the
ability to rapidly and inexpensively source traditional, custom, and bulk
compounds in the gram to kilogram scale. We complete our sourcing
using stringent quality control standards with state-of-the-art methods.
18 19

11. COMPREHENSIVE SOLUTIONS FOR
COMPLEX PROTEIN NETWORKS
PROTEOSTASIS
Protein homeostasis or ‘proteostasis’ is the process that regulates
proteins within the cell in order to maintain the health of both the cellular
proteome and the organism itself.
Proteostasis involves a highly complex interconnection of pathways
that influence the fate of a protein from synthesis to degradation. As
individual components are affected, the others adjust accordingly to
maintain normal function. Disruption of one or more of these proteostasis
influencers can manifest in pathologies such as Alzheimer’s disease,
cancer, and diabetes.
To fuel your proteostatis research, Enzo Life Sciences has an unrivaled
portfolio of ubiquitin/proteasome and heat shock protein research
reagents through our acquistion of Biomol International and Stressgen®
Bioreagents.Ourdevelopmenteffortswithintheareasofproteinsynthesis,
modification, and degradation continue to produce cutting-edge tools for
dissecting key biological processes at the forefront of academic discourse
in the proteostasis field. This commitment is evidenced most recently by
first-to-marketimmunoassays,cellbasedassays,andcompoundlibraries
for autophagy, an emerging pathway of interest in cellular regulation of
protein turnover and its relation to disease.
Ub
Ub
Ub
Ub
Ub
Ub Ub
Ub
Ub
Ub
Ub
Ub
Ub
Ub
Ub
Ub
Lysosome
Proteasome
Aggresome
Autophagy
Aggregate/
Inclusion Body
Misfolded Protein
Aggregated Protein
Chaperone
Mediated AutophagyLAMP2A
HSC70
HDAC6
Ub
Ub
Ub
Ub
Ub
Ub
Ub
Ub
Ub
Ub
Ub
Post-Translational Modification
SUMO-Qapture™-Q Kit
UbiQapture™-Q Kit
Fluor de Lys®
HDAC & Sirtuin Assay Kits
Ubiquitin & SUMO Antibodies
Degradation
Cyto-ID®
Autophagy Detection Kit
Screen-Well®
Autophagy Compound Library
Lyso-ID®
Cytotoxicity & Detection Kits
MMP Drug Discovery Kits
Proteasome ELISA & Purification Kits
Protein Synthesis & Folding
Stressgen®
HSP70 ELISA Kits
ProteoStat®
Aggresome Detection Kit
ProteoStat®
Amyloid Plaque Detection Kit
Heme Oxygenase ELISA Kit
Screen-Well®
REDOX Library
20 21
Figure adapted from: Kraft, et al.; Nat Cell Biol. 12, 836 (2010).

12. EPIGENETICS
UNRAVEL THE CODE
WITHIN THE CODE
Acetylation
Acetyltransferase Activity Assays
p300/CBP Inhibitors, Activators, and Antibodies
Deacetylation
Fluor de Lys®
HDAC & Sirtuin Assays
Color de Lys™
HDAC & Sirtuin Assays
HDAC Antibodies
Resveratrol
Tubacin
Methylation
Methyltransferase Activity Assays
CARM1, PRMT, and SET7/9 Active Enzymes
Mono- & Tri-methyl-specific Histone Antibodies
Sinefungin
Demethylation
LSD1 Fluorimetric Drug Discovery Kit
LSD1 Active Enzyme
Ubiquitinylation
UbiQapture™-Q Kit
In Vitro Ubiquitinylation Kit
Mono- & Polyubiquitinylation Antibodies
Phosphorylation
Phospho-specific Histone Antibodies
Aurora Kinase Active Enzymes & Inhibitors
SUMOylation
SUMO & PolySUMO-Qapture®
Kits
SUMO-Qapture-T®
Kits
In Vitro SUMOylation Kit
SUMO 1 & SUMO 2/3 Antibodies
Epigenetic modulation of gene expression is one component of the
proteostasis network, and is a focus of Enzo’s development efforts
supported by our expertise in post-translational modification biology and
high-quality manufacturing of enzyme activity assays, biochemicals,
antibodies, proteins, and peptide synthesis. Our epigenetics portfolio is
focused on the enzymology of epigenetic regulation including HDACs,
sirtuins, HATs, methyltransferases, and demethylases.
Our revolutionary Fluor de Lys®
HDAC and Sirtuin assays allow
simple, nonradioactive measurement of deacetylase activity amenable
to automated platforms. These assays are founded upon an industry-
leading portfolio of active enzymes and high-purity peptide substrates to
deliver the sensitivity needed when dissecting epigenetic pathways. In
support of screening efforts, our chemists have curated an Epigenetics
compound library, a collection of 43 biochemicals with defined activity
against epigenetic regulating enzymes, each of which can be supplied
individually upon request. Our portfolio of reagents also includes
antibodies for the detection of key epigenetic-regulating enzymes and
substrates, including modification-specific antibodies for methylated,
phosphorylated, or acetylated epitopes.
22 23

13. IMMUNOASSAY &
ASSAY DEVELOPMENT
FLEXIBLE FORMATS FOR
BIOMARKER DETECTION
Efficient Quenching of
Donor Fluorescence
Generation of
Fluorescent Signal
Donor Quencher
HRP
P
O-Ac-ADP-ribose
NAD+ H2O
NH
CO
CH3
CO
CH3
O-
+
NH3
+
+
NAM
SIRTs
(Class III HDACs)
(Classes I & II)
HDACs
Developer
Fluor-de-Lys®
Fluor-de-Lys®
Fluor-de-Lys®
Enhancer
Light Light
Enzo Life Sciences offers hundreds of immunoassay and enzyme activity
assay kits in a variety of formats to service the biomarker and drug
discovery assay markets. Our assay offering includes both competitive
immunoassaysforpeptidesandsmallmoleculessuchascyclicnucleotides
and eicosanoids, and immunometric “sandwich” ELISA format assays
for large protein analytes including cytokines, cell stress proteins, and
signaling pathway regulators. As scientists and manufacturers of kits,
we understand the critical nature of your research. Each of our assay kits
undergoes rigorous testing to ensure high precision and accuracy while
deliveringthesensitivityandspecificitytodetectbiologicallyrelevantlevels
of analyte. Our industry-proven manufacturing capabilities guarantee you
will obtain reproducible results, day-after-day and lot-after-lot.
Our decades of experience in the design and manufacture of active
enzymes and their substrates supports development of an ever-expanding
portfolio of biochemical assays. Our menu of scalable enzyme activity
assaysincludes kits for epigenetic modulation (HDAC, HAT, Sirtuin), matrix
metalloproteinases (MMPs) and caspase activity, as well as phospho-
specific antibody-based kinase assays. These assays exemplify the
synergy in our capabilities, assembling small peptide, active recombinant
protein synthesis, fluorescent labeling and detection technologies, and
antibody development into robust, reproducible assay kits.
Well Coated with Affinity Purified
Antigen Specific Antibody
Sample Antigen
1) Wash
2) Add Antibody
3) Incubate
1) Incubate
Sample Antigen
Substrate
COLOR
Well Coated with
Affinity Purified Antibody
Wash
Primary Antibody
Sample Antigen
AP - Antigen Conjugate
SubstrateAP
AP AP
COLOR
AP Sample Antigen
AP - Antigen Conjugate
AP
24 25
Competetive ELISA Format for Small Molecules & Peptides
cAMP & cGMP ELISA Kits
Eicosanoid ELISA Kits
Steroid Hormone ELISA Kits
Oxytocin, Vasopressin, and Angiotensin ELISA Kits
PEGylated Protein ELISA Kit
Immunometric ELISA Format for Large Molecules
Stressgen®
HSP & Heme Oxygenase ELISA Kits
Cytokine & Chemokine ELISA Kits
p53, Survivin, and Bcl-2 ELISA Kits
NBR1 & p62 Autophagy ELISA Kits
Dkk-1, β-Catenin, and GSK-3β ELISA Kits
FRET-Based Format
MMP Drug Discovery Assay Kits
Caspase Activity Assay Kits
Modified Substrate-Based Format
Fluor de Lys®
HDAC & Sirtuin Assay Kits (Fluorometric)
Color de Lys™
HDAC & Sirtuin Assay Kits (Colorometric)
ChemiLum de Lys®
HDAC & Sirtuin Assay Kits (Chemiluminescent)
Antibody-Based Format
PKA, Akt, and PKC Activity Assays (Colorimetric)
Immunoassays
Enzyme Activity Assays

14. Formats
• Small & large molecule non-radioactive detection
• Plate-based single analyte ELISA/EIA
• Bead-based multiplex platform for flow cytometry
• Heterogeneous & homogeneous formats
• Biomarker assay development
• Enzyme activity assays
• Complex-specific antibody pairs
Consultation and Proposal
• Feasibility
• Assay format
• Detection method
• Reagent availability
• Assay specifications
• Suitable matrices
• Scale-up needs
• Staged project schedule & timeline
Reagent Capabilities
• Recombinant protein expression & purification
• Small molecule synthesis
• Peptide synthesis
• Custom antibody development
• Conjugation
Manufacturing Capabilities
• State-of-the-art manufacturing facility
• Automated high precision plate coating
• Solid/strip plates in 96 or 384 well format
• Automated packaging & filling equipment
• Custom packaging & bulk fills available
FORMAT CHOICES
Our broad expertise gives you the
flexibility to choose the platform that
meets the needs of your assay
REAGENT DEVELOPMENT
If a reagent exists, our global sourcing
team can find the best one. If not, our
expertise is there to develop one for you
ASSAY FEASIBILITY
Critical reagents are first tested to
determine feasibility. Qualified reagents
are then validated to develop a final assay
protocol and SOP
SCALABLE MANUFACTURING
Our industry-proven assay manufacturing
capabilities guarantee your assay is both
robust and reproducible
PROJECT DESIGN
Consultation from a dedicated project
manager to create a comprehensive
proposal
Validation Parameters
• Analyte & reagent stability
• Range of quantification
• Limit of quantification
• Limit of detection
• Recovery matrix effects
• Selectivity
• Specificity
• Dilution linearity
• Parallelism
• Intra-assay & inter-assay reproducibility
CUSTOMIZED SOLUTIONS FOR
YOUR WORKFLOW
CUSTOM
SERVICES
A single, static catalog cannot hold all the necessary tools to meet the
needs of an ever-changing industry. Our flexibility and access to our
internal research and development resources enable our customers
access to a wider, more useful, and ever-expanding toolbox.
Choose what you need when you need it.
Whether you require complex assay development or synthesis of novel
reagents, our innovative and experienced team of scientists brings
professional project management skills and scientific understanding
to fulfill your needs on time and to your specifications. Our state-of-
the-art laboratories guarantee production and validation of your custom
product at the highest level.
The reagent manufacturing capabilities that bring strength to our assay
development services are also available on a contract basis. We do
not serve the masses with high-throughput assembly-line reagents
manufacturing. We do offer high quality, high purity reagents to
customers who care that the reagents they contract us to produce are
designed, validated, and manufactured with the scientific know-how
inherent in our decades of experience in the life sciences reagents
business.
26 27

15. “The SMN ELISA kit addresses a critical gap in SMA research and is expected to
significantly accelerate SMA therapeutics development. Development and distribution of
this system could not have happened without the successful collaboration with Enzo and
contributions from the SMA research community.”
Karen S. Chen, Ph.D., Chief Scientific Officer, SMA Foundation
SMN ELISA from concept to market
Spinal Muscular Atrophy (SMA) Foundation
Develop a quantitative ELISA for Survival Motor Neuron (SMN) protein
in human and mouse PBMC lysates, to serve as an alternative to more
time-consuming, semi-quantitative Western blotting.
Following screening of commercially available, privately licensed, and
internally developed recombinant protein standards and detection
antibodies, a workable assay was deemed to meet feasibility requirements
for sensitivity and specificity. Pilot lot kits were manufactured and
distributed to researchers within the SMA Foundation network of academic
and industry-based SMN-focused research groups. Sample preparation
protocols and sample type validation efforts were expanded as additional
needs were determined through end-user feedback.
A fully validated ELISA (ADI-900-209) and related SMN reagents are
now commercially available to the SMA research community. The ELISA
sensitivity is 50pg/mL of SMN, and the human SMN standard (developed
in-house) displays parallelism with native human and mouse SMN protein
in cell lysate samples.
COLLABORATION:
NEED:
CLIENT:
RESULT:
28 29
MARKET
STORIES
The Synergy of Our Combined
Expertise: HDAC Profiling
Histone deacetylases (HDACs) are implicated in many human diseases,
and are a frequent target for the cancer drug discovery market. Our
expertise in organic chemistry and production of high-purity enzymes
and unique fluorescent substrates combine to make possible HDAC
inhibitor profiling in a high throughput screening format. This synergy
is demonstrated by identification of the proprietary Enzo compound
BML-281 as a potent and selective inhibitor of the HDAC6 enzyme.
Screening was performed using Fluor de Lys®
Green substrate and
developer along with active recombinant human HDAC enzymes.
This flexible fluorescent assay technology is suitable for use with
automated plate readers such as the Biotek Synergy™ H4 Multi-Mode
Microplate Reader for high throughput assessment of enzyme kinetics
and inhibition.
BRINGING IT ALL TOGETHER

16. ANIMAL HEALTH
The Science of Conservation
With thousands of species each year listed as endangered or threatened,
the work done by biologists around the world to understand and preserve
animal health is never ending. Reproduction studies and stress are two
of the most common concerns today, as each species has its unique
set of challenges that affect the biology and stresses related to rescue
efforts, rehabilitation, and mating.
The challenges of collecting biological samples from a 2-ton Walrus or
Polar Bear are obvious. Biologists responsible for their care spend great
time and effort collecting and processing urine, feces, blood, saliva,
feathers, and fur. For nearly twenty years scientists have trusted Enzo’s
steroid hormone ELISA kits to deliver sensitivity and reproducibility for
the analysis of their samples, and have accessed our comprehensive
support team to optimize input sample types and share feedback.
Our most popular ELISAs for the quantification of 17β-estradiol,
progesterone, testosterone, cortisol, and corticosterone have been cited
in numerous publications and protocols shared by zoos, aquariums, and
conservation scientists worldwide, allowing us to create and share an
extensive database of knowledge for methods that benefit creatures
both large and small.
30
MARKET
STORIES
BRINGING IT ALL TOGETHER
© copyright 2011 Enzo Life Sciences, Inc.
M30-APOPTOSENSE and EPIDEATH are registered trademarks of Peviva AB. AGILENT is a registered trademark of Agilent Technologies, Inc.
All other trademarks and registered trademarks are property of Enzo Life Sciences, Inc.

17. Life Sciences
Therapeutics
Biochem
®
®
For local distributors and detailed product information visit us online:
www.enzolifesciences.com
EUROPE/ASIA
Enzo Life Sciences (ELS) AG
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Phone: +41 61 926 8989
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info-eu@enzolifesciences.com
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Enzo Life Sciences Inc.
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Toll-Free: 1.800.942.0430
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scientists enabling scientists.